CHARACTERIZATION AND HYPOGLYCEMIC STUDY OF ISOLATED NATURAL POLYMER FROM THE STEM OF MANILKARA HEXANDRA (ROXB.) DUBARD

Objective: In the present study, the isolated natural polymer from the stem of Manilkara hexandra and their physiochemical parameters were investigated. It is further involved in hypoglycemic studies. Methods: The gum exudates were screened for phytochemicals, physicochemically analyzed for solubility, pH, total ash, moisture content, acid insoluble ash, water soluble ash, sulfated ash, flow property, and it is characterized by Fourier Transform Infrared analysis (FT-IR), Scanning Electron Microscope (SEM), X-ray diffraction analysis (XRD), Nuclear Magnetic Resonance (NMR) studies (13Carbon and 1Proton) and it was examined in vitro studies by hypoglycaemic activity. Results: The isolated gum extracted from the stem of Manilkara hexandra (Roxb.) Dubard ash values were found to be low. The gum is found to be hygroscopic in nature due to its high moisture content (0.9131±0.03). Fourier Transform Infrared (FT-IR) spectra show relevant functional groups for gum, which is further confirmed by resonance spectral studies. The X-ray diffraction (XRD) pattern shows that the gum is amorphous as well as crystalline in nature. Scanning Electron Microscope (SEM) image confirms that the gum particles have irregular size and shape. Sugar composition analysis by Thin Layer Chromatography indicated the presence of rhamnose, arabinose. The in vitro study of hypoglycemic activity shows the best report compared with the standard. The experimental evidence offers scope to use this natural polymer in the food and pharmaceutical industry. Conclusion: The isolated natural polymer shows good result in hypoglycemic studies compared with standard

ISOLATION AND CHARACTERIZATION OF ANTIOXIDANT (FLAVONE-3-RUTINOSIDE, 3, 3’, 4’, 5, 7-PENTAHYDROXY) FROM LEAVES OF MELIA DUBIA

Objective: Various antioxidants are extensively found at different levels in many medicinal plants. This study mainly focuses on identification and separation of antioxidant from the leaf extract of Melia dubia. Materials and Methods: Soxhlet extraction adopted for extraction using solvents, namely hexane, chloroform, ethyl acetate, 70% Ethanol, methanol, and water. The antioxidant capacity of six extracts was analyzed by quick and convenient 1, 1-diphenyl-2-picrylhydrazyl method. The compound responsible for high scavenging activity was isolated and separated by chromatography techniques such as thin-layer chromatography and high-performance liquid chromatography. Antioxidant was characterized by UV-visible, Fourier-transform infrared (FT-IR), H1-nuclear magnetic resonance (NMR), C13-NMR, and mass spectroscopy. Results and Discussion: On extraction, water extract shows the highest yield percentage followed by methanol and 70% ethanol. Based on our results, IC50 value among the extracts, methanol and 70% ethanol, showed high antioxidant activity. Antioxidant which is commonly present in the methanol and 70% ethanol extract is flavone-3-rutinoside, 3, 3’, 4’, 5, 7-pentahydroxy. Conclusion: The occurrence of the antioxidant, i.e. rutin flavonoid was identified in the study, and the biological activity of this compound will determine in future work

ISOLATION OF 2-CHLOROBENZIMIDAZOLE FROM MELIA DUBIA LEAF EXTRACT AND ITS STRUCTURAL CHARACTERISATION

Objective: Natural products from medicinal plants, either as isolated compounds or as standardized plant extracts exhibit promising source of medicinal activity against various diseases. The aim of the present work was to make an attempt of isolation of bioactive principle and characterization of the isolated compound, from the medicinal plant Melia dubaiMethods: The extraction was done by a cold percolation method and the compound was separated and isolated by chromatography technique such as a thin layer chromatography (TLC), column chromatography and high-performance liquid chromatography (HPLC). The isolated compound was crystallized and the structural characterization of the isolated compound was made using UV-Visible, FT-IR, 1H-NMR, GC-MS and MS techniques which confirmed the structure of the isolated compound.Results: The separated and isolated compound was characterized by both physical and spectral methods like Ultraviolet-Visible spectroscopy (UV-Visible), Fourier transform infrared spectroscopy (FT-IR), Proton Nuclear Magnetic Resonance Spectroscopy (1H-NMR), Gas chromatography-mass spectrometry (GC-MS), and Mass spectrometry(MS). Based on the studies, organizational characteristics of one bioactive principle were deciphered. The results revealed that the isolated species is 2-chlorobenzimidazole and it agreed well with the reported value and spectra for 2-chlorobenzimidazole.Conclusion: The above results obtained in this research work clearly indicated the promising occurrence of 2-chlorobenzimidazole in Media dubia plant leaves. The future scope of these studies may guide us to view the biological activity of the isolated compound

Separation, identification and anti-Breast cancer efficacy of anthocyanin of Melia dubia bark

Numerous antioxidants were extensively found in many plants at different levels which are associated with the therapeutic importance of medicinal plants. The therapeutic impact of the traditional plants Melia dubia's leaves was previously thoroughly explored. The current investigation aimed to examine the biological application of natural antioxidants in the bark extract of Melia Dubia and their effect on breast cancer cells by MTT test. Preliminary phytochemical analysis of six extracts of Melia dubia bark was tested. Anthocyanine compound MDB-1 (isolated compound) is separated from the potential extract (methanol) by combined chromatography methods. On In-vitro analysis, the IC50 value of MDB-1 on the MCF7 cell line is 18.32 µg/ml which exhibits a cytotoxicity effect on the breast cancer cells. The isolated compound MDB-1 will be further used to develop drug formulations to overcome world challenges in cancer treatment