Fishing for the Virome of Tropical Tuna

International audienc

Fishing for the Microbiome of Tropical Tuna

International audienc

Analysis of the effect of covariates on survival of oysters in the Thau lagoon by a Cox proportional hazards model.

<p>For each covariate, the following elements are provided: its parameter estimate and the corresponding instantaneous hazard ratio and its standard error (SE); the χ² statistic (with 1 degree of freedom for all tests except for areas where it was 3) and the resulting p value for the type II test from the complete model. Covariates are the presence or not of OsHV-1 DNA in oysters (on 6 April), their triacylglycerol and carbohydrate reserves (on 6 April), and the relative variation of these energetic reserves (Δ) between 6 and 16 April.</p

Summary of multiple regression analyses using fatty acid food web markers in oysters as explanatory variables and triacylglycerol and carbohydrate (measured on 6 April and Δ) as response variables.

<p>All variables were measured in oysters sampled in Thau Mediterranean lagoon.</p

Energetic reserves of oysters in the Thau lagoon.

<p>(A, C) Kriged maps of the triacylglycerol and carbohydrate levels in oysters in the Mediterranean Thau lagoon measured on 6 April when seawater temperature reached 16°C, and 16 April at the beginning of the mass mortality phenomenon. Black points represent sampling sites, areas with grey boxes represent individual bivalve farms and dashed rectangles correspond to the three farming areas. (B, D) Triacylglycerol and carbohydrate levels of oysters as a function of time and area in the Thau lagoon. Letters indicate significant differences. Data are means ± SD.</p

Sampling sites (black crosses along with non-continuous numbering) located in the Thau lagoon.

<p>Areas with grey boxes: bivalve farms; red circle: temperature probe.</p

Survival of oysters in the Thau lagoon.

<p>(A) Kriged map of the mean survival time of oysters in the Thau Mediterranean lagoon. Mean survival time was measured as days from 6 April, when seawater temperature reached 16°C. Black points represent sampling sites, areas with grey boxes represent bivalve farms and dashed rectangles correspond to the three farming areas. (B) Left axis: survival functions of oysters for each area of the lagoon. Right axis: evolution of seawater temperature during the period of study. Temperature was recorded continuously at one location within the bivalve farming area of Marseillan (dotted line), and punctually at all locations (red circles, data are means ± SD).</p

Detection of OsHV-1 DNA according to areas and time in oysters deployed in the Thau lagoon.

<p>Data are number of pearl nets containing the sampled oysters (n = 10 pooled animals for each analysis).</p