Evaluation of the correlation between serum lipid characteristics of obese subjects and ADIPOQ gene rs266729 polymorphism in Chaharmahal and Bakhtiari Province of Iran

Obesity is a multifactorial disorder that is influenced by various factors such as behavior, diet, environment, metabolic and genetic. This disease is the result of an imbalance between energy absorption and expenditure. Mutations in genes that are responsible for appetite control and metabolism are considered as the genetic component of obesity. Adiponectin protein is one of the most effective adipokines in regulating the body's energy homeostasis and fat storage, which is expressed by the ADIPOQ gene and secreted from white adipose tissue. The concentration of this protein in the blood decreases in obesity. In this study, the relationship between rs266729 polymorphism in the ADIPOQ gene with the level of biochemical parameters such as total cholesterol and triglyceride and HDL and LDL in the blood of obese people in Borujen (a city in Iran) was investigated. This study was performed on 100 people who referred to the Tamin Ejtemaee clinic due to obesity problems in Borujen. In this study, the ARMS-PCR method was used to determine the genotype of individuals. Based on the results of this study, no significant relationship was found between biochemical parameters including total cholesterol, triglyceride, and LDL with rs266729 polymorphism genotypes in ADIPOQ gene in obese subjects. We concluded that rs266729 polymorphism cannot be useful as an index parameter for predispose genotype for imbalance in total cholesterol, triglyceride, and LDL levels in a person

Up-regulation of miR-21 decreases chemotherapeutic effect of dendrosomal curcumin in breast cancer cells

Objective(s): Despite the good results of anticancer activities by curcumin, there are some hurdles that limit the use of curcumin as an anticancer agent. Many methods were examined to overcome this defect like the use of the dendrosomal curcumin (DNC). There is increasing evidence that miRNAs play important roles in biological processes. In this study, we focus on the roles of microRNA-21 in the anti-cancer effects of DNC in breast cancer. Materials and Methods: Also, we have used different methods such as MTT, apoptosis, cell cycle analysis, transwell migration assay and RT-PCR to find out more. Results: We observed that miR-21 decreased apoptotic cells in both cells (from 6.35% to 0.34 % and from 7.72% to 1.32% orderly) and DNC increased it. As well as, our findings indicated that cell migration capacity was increased by miR-21 over expression and was decreased by DNC. The combination of miR-21 vector transfection and DNC treatment showed lower percentage of apoptotic cells or a higher level of penetration through the membrane compared with DNC treatment alone. Furthermore, DNC induced a marked increase in the number of cells in sub G1/G1 phase and a decrease in G2/M phase of the cell cycle in both; but, we observed reverse results compared it, after transfection with miR-21 vector. Conclusion: We observed that miR-21 suppress many aspects of anti-cancer effects of DNC in breast cancer cells, it seems that co-treatment with DNC and mir-21 down-regulation may provide a clinically useful tool for drug-resistance breast cancer cells