Transgenic studies reveal the positive role of LeEIL-1 in regulating shikonin biosynthesis in Lithospermum erythrorhizon hairy roots

Time-course accumulation of shikonin in four typical hairy root lines. Value of Ei-19 or EO-13 is significantly different from that of the control line WT-1 or EV-9 at each time point from 3 to 12 days, respectively (Student’s t-test, P < 0.05). (TIF 125 kb

MISO: Legacy-compatible Privacy-preserving Single Sign-on using Trusted Execution Environments

Single sign-on (SSO) allows users to authenticate to third-party applications through a central identity provider. Despite their wide adoption, deployed SSO systems suffer from privacy problems such as user tracking by the identity provider. While numerous solutions have been proposed by academic papers, none were adopted because they require modifying identity providers, a significant adoption barrier in practice. Solutions do get deployed, however, fail to eliminate major privacy issues. Leveraging Trusted Execution Environments (TEEs), we propose MISO, the first privacy-preserving SSO system that is completely compatible with existing identity providers (such as Google and Facebook). This means MISO can be easily integrated into existing SSO ecosystem today and benefit end users. MISO also enables new functionality that standard SSO cannot offer: MISO allows users to leverage multiple identity providers in a single SSO workflow, potentially in a threshold fashion, to better protect user accounts. We fully implemented MISO based on Intel SGX. Our evaluation shows that MISO can handle high user concurrency with practical performance.Comment: Accepted version of IEEE European Symposium on Security and Privacy (Euro S&P 2023

Overview of Talkative Power Conversion Technologies

Power Line Communication (PLC) and Simultaneous Wireless Information and Power Transfer (SWIPT) are popular examples of applications, where power and information are transmitted simultaneously. In most cases, different devices have been used to generate the power and communication signals independently. Usually, the information is superimposed onto the power signal using an external modulator. Recently, power converters have been used to generate pulses not just for power conversion but also for information transfer simultaneously. This approach represents a fundamental difference because it exploits the signal processing potential of Pulse Width Modulation (PWM), where for power conversion only the average pulse width is used, while the information is represented by the harmonics instead of being filtered out, as typically done in power electronics. The concept of embedding information into the switching ripple generated by a switched-mode power converter has recently been dubbed Talkative Power (TP), in this paper referred as Talkative Power Conversion (TPC). TPC technologies are reviewed in non-isolated and isolated converters in this paper. Potential applications include visible light communications, battery management systems, switching reluctance generators/motors, microgrids, and wireless electric vehicle charging stations. Finally, trending topics are identified

Differential Assembly and Shifts of the Rhizosphere Bacterial Community by a Dual Transgenic Glyphosate-Tolerant Soybean Line with and without Glyphosate Application

Modern agriculture has gained significant economic benefits worldwide with the use of genetically modified (GM) technologies. While GM crops provide convenience to humans, their biosafety has attracted increasing concern. In this study, the Illumina MiSeq was used to perform a high-throughput sequencing of the V3-V4 hypervariable regions of 16S rRNA gene (16S rDNA) amplicons to compare the rhizosphere bacterial communities of the EPSPS/GAT dual transgenic glyphosate-tolerant soybean line Z106, its recipient variety ZH10, and Z106 with glyphosate application (Z106G) during flowering, seed filling, and maturing stages under field settings. At each of the three stages, the alpha and beta diversity of rhizosphere bacterial communities revealed no significant differences between ZH10, Z106, and Z106G. However, some bacterial taxa demonstrated a greater proportional contribution, particularly the nitrogen-fixing rhizobium Ensifer fredii, in the rhizospheric soil of Z106 at the seed filling and maturing stages, when compared to ZH10 and Z106G. The present study therefore suggests that the EPSPS/GAT dual transgenic line Z106 and exogenous glyphosate application have a minimal effect on the composition of the soybean rhizosphere bacterial community but have no impact on the structure of the rhizosphere microbial community during a single planting season

Temporal and spatial analysis of anthropogenic mercury and CO2 emissions from municipal solid waste incineration in China: Implications for mercury and climate change mitigation

The contribution of municipal solid waste incineration (MSWI) to anthropogenic mercury and CO2 emissions have become increasingly important over the past decade. This study developed an inventory of anthropogenic mercury emissions and CO2 emissions during the period of 2014–2020, of MSWI process in China using a bottom-up inventory at the plant level. Overall, national MSWI anthropogenic mercury emissions increased from 2014 to 2020 by province. It was estimated that total 8321.09 kg of anthropogenic mercury emissions from 548 MSWI plants were scattered in 31 provinces of mainland China in 2020. The average intensity of mercury emission in China was 0.06 g·t−1 in 2020, which was much lower than the pre-2010 level. Furthermore, the increased CO2 emission generated by MSWI from 2014 to 2020 is 1.97 times. Anthropogenic mercury emissions and CO2 emissions were concentrated mainly in developed coastal provinces and cities. The general uncertainty of national mercury emissions and CO2 emissions was estimated to be −123% to 323% and −130% to 335%, respectively. Furthermore, future emissions were predicted from 2030 to 2060 based on different scenarios of the independent and collaborative effects of control proposals, the results indicate that the enhancement of advanced air pollution control technologies and effective management of MSWI represent pivotal factors in realizing future reductions in CO2 and mercury emissions. The findings will supplement those for mercury and CO2 emissions, and be useful for relevant policy-making and to improve urban air quality, as well as human health

Data_Sheet_2_Differential assembly of root-associated bacterial and fungal communities of a dual transgenic insect-resistant maize line at different host niches and different growth stages.xlsx

Transgenic technology has been widely applied to crop development, with genetically modified (GM) maize being the world’s second-largest GM crop. Despite the fact that rhizosphere bacterial and fungal populations are critical regulators of plant performance, few studies have evaluated the influence of GM maize on these communities. Plant materials used in this study included the control maize line B73 and the mcry1Ab and mcry2Ab dual transgenic insect-resistant maize line 2A-7. The plants and soils samples were sampled at three growth stages (jointing, flowering, and maturing stages), and the sampling compartments from the outside to the inside of the root are surrounding soil (SS), rhizospheric soil (RS), and intact root (RT), respectively. In this study, the results of alpha diversity revealed that from the outside to the inside of the root, the community richness and diversity declined while community coverage increased. Morever, the different host niches of maize rhizosphere and maize development stages influenced beta diversity according to statistical analysis. The GM maize line 2A-7 had no significant influence on the composition of microbial communities when compared to B73. Compared to RS and SS, the host niche RT tended to deplete Chloroflexi, Gemmatimonadetes and Mortierellomycota at phylum level. Nitrogen-fixation bacteria Pseudomonas, Herbaspirillum huttiense, Rhizobium leguminosarum, and Sphingomonas azotifigens were found to be enriched in the niche RT in comparison to RS and SS, whilst Bacillus was found to be increased and Stenotrophomonas was found to be decreased at the maturing stage as compared to jointing and flowering stages. The nitrogen fixation protein FixH (clusters of orthologous groups, COG5456), was found to be abundant in RT. Furthermore, the pathogen fungus that causes maize stalk rot, Gaeumannomyces radicicola, was found to be abundant in RT, while the beneficial fungus Mortierella hyalina was found to be depleted in RT. Lastly, the abundance of G. radicicola gradually increased during the development of maize. In conclusion, the host niches throughout the soil-plant continuum rather than the Bt insect-resistant gene or Bt protein secretion were primarily responsible for the differential assembly of root-associated microbial communities in GM maize, which provides the theoretical basis for ecological agriculture.</p

Additional file 6: Table S1. of Transgenic studies reveal the positive role of LeEIL-1 in regulating shikonin biosynthesis in Lithospermum erythrorhizon hairy roots

Primers for LeEIL-1 cDNA cloning and vector construction and verification. (DOC 39 kb