Structural studies of capsular polysaccharide of type XII diplococcus pneumoniae

The capsular polysaccharide of Diplococcus pneumoniae Type XII contains residues of -glucose and -galactose in a molar ratio of 2:1. The methylated polysaccharide yielded upon hydrolysis 2,3,4,6-tetra- and 3,4,6-tri-O-methyl--glucose and 2,3,4,6-tetra-O-methyl--galactose as the only neutral methyl sugars. Periodate oxidation of the polysaccharide resulted in destruction of all neutral sugars and immunochemical activity against rabbit antisera. Periodate oxidation of the methyl O-methylglycosides obtained after hydrolysis of the methylated polysaccharide indicated that at least 30% of the -fucosamine residues are substituted at C-4 in the polysaccharide. It is concluded that the polysaccharide consists of a hexosamine backbone that is substituted by -galactosyl and kojibiosyl side-chains. The proposed terminal -galactosyl residues apparently are sterically hindered from interacting with several -galactose-binding proteins.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/22259/1/0000696.pd

Quantitative determination of reducing sugars, oligosaccharides, and glycoproteins with [3H]borohydride

A very simple and direct radioactive isotope method has been described for the quantitative determination of reducing sugars. The method can be adapted to give any degree of sensitivity desired. The procedure is of general applicability to the determination of a large class of sugars. The stoichiometry of the reaction makes it a comprehensive procedure which greatly facilitates the analysis of sugars, oligosaccharides, and glycoproteins after acid and enzymic hydrolysis. Examples of the versatility of the method are given.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/33817/1/0000074.pd

Isolation and characterization of a glycopeptide from human senescent erythrocytes

A glycopeptide (called "senescence-factor glycopeptide", SF-G) has been isolated from a tryptic digest of human erythrocytes by specific adsorption and elution from immobilized peanut lectin. SF-G was detectable in old but not in young erythrocytes isolated from the same unit of blood. It is present in small quantities, O-glycosyl type, being released by an endo-N-acetyl-[alpha]--galactosaminidase.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/25133/1/0000566.pd