230 research outputs found

    SELF SERVICE TECHNOLOGIES SPEAK FOR THEMSELVES

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    The paper analysis the main criteria for successful self service technologies. Self service technologies are changing the way customers interact with companies. As a result, a lot of firms are developing innovative self service technologies with hope that their customers are satisfied. In order for a self service technology to succeed in making customers happy, companies must learn and know what drives customer satisfaction. This paper examines what a firm should consider in order to encourage customers to at least try, and eventually adopt, the self service technology offered by a firm into the customer's regular routine. Factors that encourage the customer to try a new self-service technology for the first time and factors impact customer satisfaction and dissatisfaction will be addressed.self service technologies, customer value, customer satisfaction

    THE INFLUENCE OF DOPC ON THE PERMEABILITY OF LIPID MEMBRANE: DETERGENT SOLUBILIZATION AND NMR STUDY

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    Abstract. There is an efficient, systemic transgene expression in many cell lines (in vitro) by using anionic liposomes, composed of equimolar amounts of 1, 2-dilauroyl-sn-glycero-3-phosphoethanolamine (DLPE), 1, 2-dioleoyl-sn-glycero-3-phospho-L-serine (DOPS) and cholesterol (CHOL). 1, 2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) is added to this liposomal formulation to increase the stability of small unilamellar vesicles in the presence of fetal calf serum (FCS). The liposomal stability was studied by monitoring the retention of a trapped marker, carboxyfluorescein (CF), in the internal liposome compartment. The liposomes containing DOPC had a lower stability in serum compared to the DOPC free liposomes. The extremely low retention in the DOPC liposome was ascribed to a formation of the inverted hexagonal phase of the liposomal membrane. A higher sodium deoxycholate detergent concentration is needed to solubilize DLPE/DOPS/CHOL (1:1:1) liposomes than DLPE/DOPC/DOPS/CHOL (1:1:1:1) liposomes. Phosphorus-31 nuclear magnetic resonance ( 31 P-NMR) is used to investigate the lamellar (L Ξ± ) β†’ hexagonal (H II ) phase transition of unilamellar lipid vesicles containing DOPC. It was found that unilamellar lipid vesicles containing DOPC are in the bilayer state at pH 4 and undergo a lamellar (L Ξ± ) β†’ hexagonal (H II ) phase transition at pH 8

    Surgeon of the year 2012

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