Radion and Higgs Signals in Peripheral Heavy Ion Collisions at the LHC

We investigate the sensitivity of the heavy ion mode of the LHC to Higgs boson and Radion production via photon-photon fusion through the analysis of the processes photon to photon photon, photon photon to b anti-b, and photon photon to g g in peripheral heavy ion collisions. We suggest cuts to improve the Higgs and Radion signal over standard model background ratio and determine the capability of LHC to detect these particles production.Comment: 16 pages, 8 eps figures. admin note: text overlap with arXiv:hep-ph/0009232 by different autho

Ultra peripheral heavy ion collisions and the energy dependence of the nuclear radius

To estimate realistic cross sections in ultra peripheral heavy ion collisions we must remove effects of strong absorption. One method to eliminate these effects make use of a Glauber model calculation, where the nucleon-nucleon energy dependent cross sections at small impact parameter are suppressed. In another method we impose a geometrical cut on the minimal impact parameter of the nuclear collision ($b_{min} > R_1 + R_2$, where $R_i$ is the radius of ion "$i$"). In this last case the effect of a possible nuclear radius dependence with the energy has not been considered in detail up to now. Here we introduce this effect showing that for final states with small invariant mass the effect is negligible. However when the final state has a relatively large invariant mass, e.g. an intermediate mass Higgs boson, the cross section can decrease up to 50%.Comment: To appear in Phys. Lett.

Hot Topics in Ultra-Peripheral Collisions

Ultra-peripheral collisions of relativistic heavy ions involve long-ranged electromagnetic interactions at impact parameters too large for hadronic interactions to occur. The nuclear charges are large; with the coherent enhancement, the cross sections are also large. Many types of photonuclear and purely electromagnetic interactions are possible. We present here an introduction to ultra-peripheral collisions, and present four of the most compelling physics topics. This note developed from a discussion at a workshop on ``Electromagnetic Probes of Fundamental Physics,'' in Erice, Italy, Oct. 16-21, 2001.Comment: 7 pages, with 3 figures. This developed from a discussion at the workshop on "Electromagnetic Probes of Fundamental Physics," Oct. 16-21, Erice, Ital

Heavy Quark Photoproduction in Ultra-peripheral Heavy Ion Collisions

Heavy quarks are copiously produced in ultra-peripheral heavy ion collisions. In the strong electromagnetic fields, c c-bar and b b-bar are produced by photonuclear and two-photon interactions; hadroproduction can occur in grazing interactions. We present the total cross sections, quark transverse momentum and rapidity distributions, as well as the Q Q-bar invariant mass spectra from the three production channels. We consider AA and pA collisions at the Relativistic Heavy Ion Collider and Large Hadron Collider. We discuss techniques for separating the three processes and describe how the AA to pA production ratios might be measured accurately enough to study nuclear shadowing.Comment: Minor changes to satisfy referees and typo fixes; 52 pages including 17 figure

Neutralization Serotyping of BK Polyomavirus Infection in Kidney Transplant Recipients

BK polyomavirus (BKV or BKPyV) associated nephropathy affects up to 10% of kidney transplant recipients (KTRs). BKV isolates are categorized into four genotypes. It is currently unclear whether the four genotypes are also serotypes. To address this issue, we developed high-throughput serological assays based on antibody-mediated neutralization of BKV genotype I and IV reporter vectors (pseudoviruses). Neutralization-based testing of sera from mice immunized with BKV-I or BKV-IV virus-like particles (VLPs) or sera from naturally infected human subjects revealed that BKV-I specific serum antibodies are poorly neutralizing against BKV-IV and vice versa. The fact that BKV-I and BKV-IV are distinct serotypes was less evident in traditional VLP-based ELISAs. BKV-I and BKV-IV neutralization assays were used to examine BKV type-specific neutralizing antibody responses in KTRs at various time points after transplantation. At study entry, sera from 5% and 49% of KTRs showed no detectable neutralizing activity for BKV-I or BKV-IV neutralization, respectively. By one year after transplantation, all KTRs were neutralization seropositive for BKV-I, and 43% of the initially BKV-IV seronegative subjects showed evidence of acute seroconversion for BKV-IV neutralization. The results suggest a model in which BKV-IV-specific seroconversion reflects a de novo BKV-IV infection in KTRs who initially lack protective antibody responses capable of neutralizing genotype IV BKVs. If this model is correct, it suggests that pre-vaccinating prospective KTRs with a multivalent VLP-based vaccine against all BKV serotypes, or administration of BKV-neutralizing antibodies, might offer protection against graft loss or dysfunction due to BKV associated nephropathy

Immunogenicity of Self-Associated Aggregates and Chemically Cross-Linked Conjugates of the 42 kDa Plasmodium falciparum Merozoite Surface Protein-1

Self-associated protein aggregates or cross-linked protein conjugates are, in general, more immunogenic than oligomeric or monomeric forms. In particular, the immunogenicity in mice of a recombinant malaria transmission blocking vaccine candidate, the ookinete specific Plasmodium falciparum 25 kDa protein (Pfs25), was increased more than 1000-fold when evaluated as a chemical cross-linked protein-protein conjugate as compared to a formulated monomer. Whether alternative approaches using protein complexes improve the immunogenicity of other recombinant malaria vaccine candidates is worth assessing. In this work, the immunogenicity of the recombinant 42 kDa processed form of the P. falciparum merozoite surface protein 1 (MSP142) was evaluated as a self-associated, non-covalent aggregate and as a chemical cross-linked protein-protein conjugate to ExoProtein A, which is a recombinant detoxified form of Pseudomonas aeruginosa exotoxin A. MSP142 conjugates were prepared and characterized biochemically and biophysically to determine their molar mass in solution and stoichiometry, when relevant. The immunogenicity of the MSP142 self-associated aggregates, cross-linked chemical conjugates and monomers were compared in BALB/c mice after adsorption to aluminum hydroxide adjuvant, and in one instance in association with the TLR9 agonist CPG7909 with an aluminum hydroxide formulation. Antibody titers were assessed by ELISA. Unlike observations made for Pfs25, no significant enhancement in MSP142 specific antibody titers was observed for any conjugate as compared to the formulated monomer or dimer, except for the addition of the TLR9 agonist CPG7909. Clearly, enhancing the immunogenicity of a recombinant protein vaccine candidate by the formation of protein complexes must be established on an empirical basis

Two-photon final states in peripheral heavy ion collisions

We discuss processes leading to two photon final states in peripheral heavy ion collisions at RHIC. Due to the large photon luminosity we show that the continuum subprocess gamma gamma -> gamma gamma can be observed with a large number of events. We study this reaction when it is intermediated by a resonance made of quarks or gluons and discuss its interplay with the continuum process, verifying that in several cases the resonant process ovewhelms the continuum one. It is also investigated the possibility of observing a scalar resonance (the sigma meson) in this process. Assuming for the sigma, the mass and total decay width values recently reported by the E791 Collaboration we show that RHIC may detect this particle in its two photon decay mode if its partial photonic decay width is of the order of the ones discussed in the literature

Guidelines for Small-Scale Production and Purification of Hepatitis B Surface Antigen Virus-Like Particles from Recombinant Pichia pastoris.

Virus-like particle (VLP)-based vaccines have been in the market since decades for preventing viral infection and have proven their usefulness also in other areas of biotechnology. Here, we describe in detail simple small-scale production and purification procedures for the generation of hepatitis B surface antigen (HBsAg) VLPs using Pichia pastoris as expression host. This protocol may also be applicable with variations to other HBsAg-based VLPs additionally carrying antigens of other pathogens