"Pockett blotting" : a method for transferring nucleic acids onto nylon membranes

We have developed a fast and efficient method for transferring nucleic acids onto nylon membranes. This method requires less DNA for transfer; no decrease in efficiency is observed after successive probing, and several gels can be processed simultaneously. We believe that this technique is of general interest in routine analysis of multiple samples in population genetic studies or in diagnosis purposes. (Résumé d'auteur

The relationships between the 5' end repeats and the largest members of the L1 interspersed repeated family in the mouse genome.

Analysis of a few large L1 elements has revealed two types of tandem repeats at the 5' end: A and F. In this study, the relationships between these repeats and a series of large L1 elements has been analysed. Most of cloned L1 repeats were shown to lack either A or F sequences at their 5' ends. F sequences are found less frequently associated than A sequences to the 5' ends of L1 and an evolutionary comparison shows that the A type was introduced more recently during the evolution of the mouse genome than the F type

3' Alu PCR: a simple and rapid method to isolate human polymorphic markers.

Microsatellites, such as (TG)n found at random throughout the genome, or as 3' extensions of Alu sequences are being increasingly used as genetic markers because of their pluriallelic character. The search for polymorphic microsatellites is time consuming, however, as it is necessary to sequence clones containing the microsatellites sequences in order to design specific PCR primers before testing for polymorphism, which does not always occur. We propose here a new approach to generate polymorphic markers, based on the amplification of microsatellites at the 3' end of Alu sequences, without the need for cloning or sequencing steps