16 research outputs found
Graft-transmissible diseases of citrus. Handbook for detection and diagnosis
available on CD-ROM accession number 36741
Separation of citrus viroids by shoot-tip grafting in vitro
Arizona 861âS1 citron (Citrus media L.) infected with a severe exocortis isolate containing four citrus viroids was used as source of tissue for shootâtip grafting in vitro. Out of 51 attempts, 25 successful grafts were obtained. Only 16 plants survived transplanting and of these 12 were viroidâfree. The viroid profile of the other four plants showed fewer viroids than the original field source. The significance of these results, as compared with previous reports on the recovery of viroidâfree plants, is discussed. The results show the usefulness of shootâtip grafting in vitro as a tool to recover single viroid isolates from complex field sources