Hubungan Kelincahan dengan Keterampilan Menggiring Bola pada Siswa Kelas V.e Sdn 006 Pangkalankerinci Kab. Pelalawan

Based on the researcher\u27s observation of students in class V,E of SDN 006 Pangkalan Kerinci Pelalawan District. Researcher found many student have deficiency of dribbling, visible when the student dribble imperfect, as the ball away from the feet, so that the ball easily captured opponent. It is influenced by physical conditions that agility. The purpose of this research is to see the correlation between agility with ball dribbling skills of Student class V.E of SDN 006 Pangkalan Kerinci Pelalawan District. Population in this research is all Student in class V of SDN 006 Pangkalan Kerinci Pelalawan District amount 22 people. Based on population that is not so large and within the limits the researchers set the whole population is used as a sample. The research sample as many as 22 people. Research instrument used suttle run test to agility and dribbling ball test. Data were analyzed by product moment correlation. Based on research results, it can be concluded as follows : that is result obtained from Student in class V.E of SDN 006 Pangkalan Kerinci Pelalawan District. Shows that there is correlation of the agility (X) with dribbling (Y) , which shows correlation rhitung = 0,462>rtabel = 0,43

Pharmacokinetic study on pradofloxacin in the dog – Comparison of serum analysis, ultrafiltration and tissue sampling after oral administration

Background: Pradofloxacin, a newly developed 8-cyano-fluoroquinolone, show enhanced activity against Grampositive organisms and anaerobes to treat canine and feline bacterial infections. The purpose of this cross-over study was to measure the unbound drug concentration of pradofloxacin in the interstitial fluid (ISF) using ultrafiltration and to compare the kinetics of pradofloxacin in serum, ISF and tissue using enrofloxacin as reference. Results: After oral administration of enrofloxacin (5 mg/kg) and pradofloxacin (3 mg/kg and 6 mg/kg, respectively), serum collection and ultrafiltration in regular intervals over a period of 24 h were performed, followed by tissue sampling at the end of the third dosing protocol (pradofloxacin 6 mg/kg). Peak concentrations of pradofloxacin (3 mg/kg) were 1.55±0.31 μg/ml in the ISF and 1.85±0.23 μg/ml in serum and for pradofloxacin (6 mg/kg) 2.71±0.81 μg/kg in the ISF and 2.77±0.64 μg/kg in serum; both without a statistical difference between ISF and serum. Comparison between all sampling approaches showed no consistent pattern of statistical differences. Conclusions: Despite some technical shortcomings the ultrafiltration approach appears to be the most sensitive sampling technique to estimate pharmacokinetic values of pradofloxacin at the infection site. Pharmacokinetics – Pradofloxacin – Ultrafiltration – Dog – Oral Administration

Effect of single intralesional treatment of surgically induced equine superficial digital flexor tendon core lesions with adipose-derived mesenchymal stromal cells: a controlled experimental trial

Background: Adipose tissue is a promising source of mesenchymal stromal cells (MSCs) for the treatment of tendon disease. The goal of this study was to assess the effect of a single intralesional implantation of adipose tissue-derived mesenchymal stromal cells (AT-MSCs) on artificial lesions in equine superficial digital flexor tendons (SDFTs). Methods: During this randomized, controlled, blinded experimental study, either autologous cultured AT-MSCs suspended in autologous inactivated serum (AT-MSC-serum) or autologous inactivated serum (serum) were injected intralesionally 2 weeks after surgical creation of centrally located SDFT lesions in both forelimbs of nine horses. Healing was assessed clinically and with ultrasound (standard B-mode and ultrasound tissue characterization) at regular intervals over 24 weeks. After euthanasia of the horses the SDFTs were examined histologically, biochemically and by means of biomechanical testing. Results: AT-MSC implantation did not substantially influence clinical and ultrasonographic parameters. Histology, biochemical and biomechanical characteristics of the repair tissue did not differ significantly between treatment modalities after 24 weeks. Compared with macroscopically normal tendon tissue, the content of the mature collagen crosslink hydroxylysylpyridinoline did not differ after AT-MSC-serum treatment (p = 0.074) while it was significantly lower (p = 0.027) in lesions treated with serum alone. Stress at failure (p = 0.048) and the modulus of elasticity (p = 0.001) were significantly lower after AT-MSC-serum treatment than in normal tendon tissue. Conclusions: The effect of a single intralesional injection of cultured AT-MSCs suspended in autologous inactivated serum was not superior to treatment of surgically created SDFT lesions with autologous inactivated serum alone in a surgical model of tendinopathy over an observation period of 22 weeks. AT-MSC treatment might have a positive influence on collagen crosslinking of remodelling scar tissue. Controlled long-term studies including naturally occurring tendinopathies are necessary to verify the effects of AT-MSCs on tendon disease

The impact of upstream and downstream processing on the quality of oil bodies of partially de-hulled sunflower seeds

Few publications on oil bodies or oleosomes seem concerned about their quality (chemical and physical) ex-vivo. This work attempts to identify the main factors (processing and pre-processing) that affect the quality/integrity of sunflower seed oil bodies recovered through a wet-milling process. The physical state of seeds during wet milling had a significant impact on the quality of the oil body suspension. Pre-soaking for 6 hours before wet milling and multiple washing with alkaline buffer (0.1M sodium bicarbonate) was performed to isolate high quality oil body suspensions. It was evident from different physical measurements such as particle size, ζ-potential and light microscopy that pre-soaking had a positive influence on the quality of oil body suspensions with no significant signs of aggregation or coalescence. It was also observed that the resultant washed oil body suspensions were highly surface charged (-28.4 ± 1.2 mV) indicating very stable suspension phase behavior. Washing oil bodies not only removes non-integral, extraneous proteins (derived from the seed matrix) but enriches the lipid content including Tocopherol (α-tocopherol: 491.6 mg/kg of washed oil bodies compared with 252.6 mg/kg crude oil bodies). Changes in the composition of oil bodies after washing have been observed before, but this research also monitored the size of oil bodies after washing, and our results indicate that certain factors can shift the distribution of droplet size. It is believed that any change in average size of droplets indicate the presence of disrupted oil bodies whose surface chemistry has changed enough to compromise their integrity on washing. The retention of droplet size on washing may, therefore, be diagnostic for the recovery of intact oil bodies. An assessment of the integrity of oil bodies recovered from sunflower seeds after accelerated aging (5 months) was carried out. Free fatty acid was more pronounced in oil rather than oil bodies, this could be due to the elimination of some of the free acid bound to oil body during washing. Although some minor variation was observed during seed aging, however, the oil bodies remained stable in the final suspension. The results indicate that oil body membrane was extremely robust under extreme conditions and the integrity of oil bodies was preserved. In addition, oil bodies obtained in this study were resistant to oxidation due to the presence of naturally occurring antioxidants (including vitamin E) associated with them.. The results indicate that the physical barrier of surface membrane protein (oelosin) protect oil bodies against pro-oxidants

Die Fl�chen vierter Ordnung hinsichtlich ihrer Knotenpunkte und ihrer Gestaltung: Mit einer Figurentafal

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High‐field (3 Tesla) MRI of the navicular apparatus of sound horses shows good agreement to histopathology

Magnetic resonance imaging and the correlation to histopathological findings of the equine palmar foot of lame horses have been described previously, using 0.27 and 1.5 T systems. Compared to these, 3 T systems provide superior spatial resolution and imaging contrast. The aim of our prospective anatomic study was to characterize the imaging anatomy of the navicular region on 3 T MRI in comparison to histopathological findings. We hypothesized that 3 T MRI allows a good visualization of the entire navicular apparatus and reliable measurements of navicular cartilage and cortical bone thickness. Twenty front feet of sound horses were examined using a 3 T MRI system. For histopathological examination, sagittal tissue sections of the navicular bones and adjacent ligaments were prepared. Alterations in magnetic resonance signal were graded for each region and compared to corresponding histological slices. Overall, there was good visualization of the anatomical detail and a very good agreement between MRI and histology for compact bone and spongiosa, good agreement for the fibrocartilage and the distal sesamoidean impar ligament, but only moderate agreement for the hyaline cartilage and the collateral sesamoidean ligament. A comparative measurement of cartilage and cortical bone thickness on magnetic resonance images and histological sections was performed. In MRI, the hyaline cartilage of the articular surface appeared significantly thinner and the fibrocartilage of the flexor surface appeared significantly thicker compared to histology. Findings indicated that MRI at a field strength of 3 T allows reliable depiction of anatomic details of the navicular apparatus