Polymorphism of Pramenka sheep hemoglobin in Central Bosnia

The study of the hemoglobin polymorphism was performed on blood samples, taken from areas inhabited by Pramenka sheep in Central Bosnia: the area of the mountain Vlašić, the upper flow of the river Vrbas and Kupres plateau. Dupska pramenka sheep inhabits the mountain Vlašić and the areas of surrounding municipalities. Privorska pramenka sheep inhabits the area around the upper flow of the river Vrbas and the mountain Vranica and Kupreška pramenka sheep inhabits Kupres plateau. HbA gene frequency was 0.31 and HbB gene frequency was 0.69 for Dupska pramenka sheep. Privorska pramenka sheep had a frequency of 0.33 for the HbA gene, and 0.67 for the HbB gene, in its population. Kupreška pramenka sheep had the lowest frequency of the HbA gene, (0.30), and therefore the maximum frequency of the HbB gene. Hemoglobin genotype frequencies for all investigated types of Pramenka sheep breed are in equilibrium. Investigated types of Pramenka sheep breed inhabit areas adjacent to each other. They are phenotypically quite similar and have a similar frequency of genotype polymorphism of hemoglobin. The difference between them is not statistically significant

Influence of different meal sizes on growth characteristics of young rainbow trout (Oncorhynchus mykiss Wal).

This paper presents the results of the effects of different sizes of meals (80%, 90%, 100%, 110% and 120% regarding the amount recommended by the food producer) on the growth characteristics of young rainbow trout (Oncorhynchus mykiss Wal.) aged from 2 to 3.5 months. The experiment was carried out at the Laboratory of Aquaculture, Faculty of Agriculture, University of Banja Luka for a period of 47 days. In the experiment, there were a total of 500 fish, divided into five groups, and placed in the 55-l aquarium with a constant flow of water and the aeration system. The main parameters of water quality were analyzed every day and they included: water temperature 12.64-12.76°C, content of dissolved oxygen 7.83-8.09 mg/l, saturation of water with oxygen 75.47-77.39% and pH value 7.224-7.268. In the end, the average individual weight and body length (±standard deviation) of young rainbow trout, regarding the experimental groups (EG), were reached: EG80 10.53±2.58 g and 9.94±0.76 cm, EG90 12.14±2.97 g and 10.45±0.86 cm, EG100 13.18±2.91 g and 10.67±0.82 cm, EG110 13.80±3.14 g and 10.82±0.78 cm, EG120 14.58±3.63 g and 10.81±1.11 cm. Statistically highly significant differences were found between the means of experimental groups (p <0,01). The coefficient of condition of young rainbow trout at the end of the experiment was similar in the treatments EG80, EG90, EG100 and EG110 (1.06 to 1.09), and it was the highest in the treatment EG120 (1,15±0,17). Generally, it can be said that increasing of the size of meals up to 10% above the recommended value does not cause significant differences, and if the increase is greater than 10%, statistically significant difference in body weight occurs

Assessment of genetic diversity of Buša cattle from Bosnia and Herzegovina using microsatellite DNA markers

The variability of 21 microsatellite loci was analyzed in order to estimate the genetic diversity of the Buša cattle breed. A total of 50 animals involved in the study were divided into two groups: Buša from eastern Herzegovina and Buša from western Herzegovina. The mean number of alleles per locus was 6.6. The average expected heterozygosities were 0.6885 and 0.6212 in the eastern and western populations, respectively. The observed heterozygosity values were 0.6579 and 0.6336, in eastern and western population, respectively. The degree of population differentiation (FST) ranged from 0.008 (for ILSTS006 locus) to 0.242 (for BM1818 locus), with a mean of 0.112. Cluster analysis showed that the two populations were clearly clustered into two distinct clades. This report represents the first genetic characterization of pure Buša cattle from Bosnia and Herzegovina. The obtained results are important for the future development of conservation and management strategies for this cattle breed

Femtosecond laser surface patterning of steel and titanium alloy

In this work, we present the results obtained by femtosecond laser processing of AISI D2 steel and the widely applicable titanium-based alloy, Ti6Al4V. Patterning the materials surfaces was done by a Ti: sapphire system at 775 nm wavelength and 200 fs pulse duration, while varying the output pulse energies and the scanning speed. The formation of laser-induced periodical surface structures were found for both materials.4th International School and Conference on Photonics, Aug 26-30, 2013, Belgrade, Serbi