FSH Injections and Ultrasonography Determine Presence of Ovarian Components in the Evaluation of Ovotesticular Disorders of Sex Development

Three infants with ambiguous genitalia and suspected ovotestes were given recombinant FSH to induce ovarian follicular development. The development of follicles in the gonadal tissue suggested the presence of ovarian tissue in two of the three infants. This method may provide a means to better characterize gonadal anatomy in patients affected by disorders of sex development (DSD). Sonographic information poststimulation provided parents with earlier and more specific education and support concerning the possible need for confirmative gonadal biopsy treatment options

Development of a spatially dispersed short-coherence interferometry sensor using diffraction grating orders

Modern manufacturing processes can achieve good throughput by requiring that manufactured products be screened by better quality control exercised at a quicker rate. This trend in the quality control of manufactured products increases the need for process-oriented precision metrology capable of performing faster inspections and yielding valuable feedback to the manufacturing system. This paper presents a spatially dispersed short-coherence interferometry sensor using diffraction orders of the zeroth and first order for a diffraction grating introduced as a new compact system configuration for surface profile measurement. In this modified design, the diffraction grating acts as the beam splitter/combiner. Diffractions for the zeroth and first orders are represented by the reference and measurement arms, respectively, of a Michelson interferometer, which reduces the optical path length. This innovative design has been proven effective for determining the step-height repeatability in the sensor range from 27 nm to 22 nm for profiles spanning the step heights of the tested specimens

Analyzing symmetry breaking within a chaotic quantum system via Bayesian inference

Bayesian inference is applied to the level fluctuations of two coupled microwave billiards in order to extract the coupling strength. The coupled resonators provide a model of a chaotic quantum system containing two coupled symmetry classes of levels. The number variance is used to quantify the level fluctuations as a function of the coupling and to construct the conditional probability distribution of the data. The prior distribution of the coupling parameter is obtained from an invariance argument on the entropy of the posterior distribution.Comment: Example from chaotic dynamics. 8 pages, 7 figures. Submitted to PR

A population search filter for hard-to-reach populations increased search efficiency for a systematic review

Objective: This paper discusses how hard-to-reach population groups were conceptualised into a search filter. The objectives of this paper are: 1) to discuss how the authors designed a multi-stranded population search filter and, 2) to retrospectively test the effectiveness of the search filter in capturing all relevant populations (e.g. homeless people, immigrants, substance misusers) in a public health systematic review. Study design and setting: Systematic and retrospective analysis via case-study. Retrospective analysis of the search filter was conducted by comparing the MEDLINE search results retrieved without using the search filter against those retrieved with the search filter. 5465 additional results from the unfiltered search were screened to the same criteria as the filtered search. Results: No additional populations were identified in the unfiltered sample. The search filter reduced the volume of MEDLINE hits to screen by 64% with no impact on inclusion of populations. Conclusion: The results demonstrate the effectiveness of the filter in capturing all relevant UK populations for the review. This suggests that well planned search filters can be written for reviews which analyse imprecisely defined population groups. This filter could be used in topic areas of associated co-morbidities, for rapid clinical searches, or for investigating hard-to-reach populations

Protocol for ACCESS: a qualitative study exploring barriers and facilitators to accessing the emergency contraceptive pill from community pharmacies in Australia

Introduction The rate of unplanned pregnancy in Australia remains high, which has contributed to Australia having one of the highest abortion rates of developed countries with an estimated 1 in 5 women having an abortion. The emergency contraceptive pill (ECP) offers a safe way of preventing unintended pregnancy after unprotected sex has occurred. While the ECP has been available over-the-counter in Australian pharmacies for over a decade, its use has not significantly increased. This paper presents a protocol for a qualitative study that aims to identify the barriers and facilitators to accessing the ECP from community pharmacies in Australia. Methods and analysis Data will be collected through one-on-one interviews that are semistructured and in-depth. Partnerships have been established with 2 pharmacy groups and 2 women's health organisations to aid with the recruitment of women and pharmacists for data collection purposes. Interview questions explore domains from the Theoretical Domains Framework in order to assess the factors aiding and/or hindering access to ECP from community pharmacies. Data collected will be analysed using deductive content analysis. The expected benefits of this study are that it will help develop evidence-based workforce interventions to strengthen the capacity and performance of community pharmacists as key ECP providers. Ethics and dissemination The findings will be disseminated to the research team and study partners, who will brainstorm ideas for interventions that would address barriers and facilitators to access identified from the interviews. Dissemination will also occur through presentations and peer-reviewed publications and the study participants will receive an executive summary of the findings. The study has been evaluated and approved by the Monash Human Research Ethics Committee

Deficiency in origin licensing proteins impairs cilia formation: implications for the aetiology of meier-gorlin syndrome

Mutations in ORC1, ORC4, ORC6, CDT1, and CDC6, which encode proteins required for DNA replication origin licensing, cause Meier-Gorlin syndrome (MGS), a disorder conferring microcephaly, primordial dwarfism, underdeveloped ears, and skeletal abnormalities. Mutations in ATR, which also functions during replication, can cause Seckel syndrome, a clinically related disorder. These findings suggest that impaired DNA replication could underlie the developmental defects characteristic of these disorders. Here, we show that although origin licensing capacity is impaired in all patient cells with mutations in origin licensing component proteins, this does not correlate with the rate of progression through S phase. Thus, the replicative capacity in MGS patient cells does not correlate with clinical manifestation. However, ORC1-deficient cells from MGS patients and siRNA-mediated depletion of origin licensing proteins also have impaired centrosome and centriole copy number. As a novel and unexpected finding, we show that they also display a striking defect in the rate of formation of primary cilia. We demonstrate that this impacts sonic hedgehog signalling in ORC1-deficient primary fibroblasts. Additionally, reduced growth factor-dependent signaling via primary cilia affects the kinetics of cell cycle progression following cell cycle exit and re-entry, highlighting an unexpected mechanism whereby origin licensing components can influence cell cycle progression. Finally, using a cell-based model, we show that defects in cilia function impair chondroinduction. Our findings raise the possibility that a reduced efficiency in forming cilia could contribute to the clinical features of MGS, particularly the bone development abnormalities, and could provide a new dimension for considering developmental impacts of licensing deficiency

A Recombinant Potato virus Y Infectious Clone Tagged with the Rosea1 Visual Marker (PVY-Ros1) Facilitates the Analysis of Viral Infectivity and Allows the Production of Large Amounts of Anthocyanins in Plants

"This Document is Protected by copyright and was first published by Frontiers. All rights reserved. It is reproduced with permission."[EN] Potato virus Y (PVY) is a major threat to the cultivation of potato and other solanaceous plants. By inserting a cDNA coding for the Antirrhinum majus Rosea1 transcription factor into a PVY infectious clone, we created a biotechnological tool (PVY-Ros1) that allows infection by this relevant plant virus to be tracked by the naked eye with no need for complex instrumentation. Rosea1 is an MYB-type transcription factor whose expression activates the biosynthesis of anthocyanin pigments in a dose-specific and cell-autonomous manner. Our experiments showed that the mechanical inoculation of solanaceous plants with PVY-Ros1 induced the formation of red infection foci in inoculated tissue and solid dark red pigmentation in systemically infected tissue, which allows disease progression to be easily monitored. By using silver nanoparticles, a nanomaterial with exciting antimicrobial properties, we proved the benefits of PVY-Ros1 to analyze novel antiviral treatments in plants. PVY-Ros1 was also helpful for visually monitoring the virus transmission process by an aphid vector. Most importantly, the anthocyanin analysis of infected tobacco tissues demonstrated that PVY-Ros1 is an excellent biotechnological tool for molecular farming because it induces the accumulation of larger amounts of anthocyanins, antioxidant compounds of nutritional, pharmaceutical and industrial interest, than those that naturally accumulate in some fruits and vegetables well known for their high anthocyanin content. Hence these results support the notion that the virus-mediated expression of regulatory factors and enzymes in plants facilitates easy quick plant metabolism engineering.This research was supported by grants BIO2014-54269-R and AGL2013-49919-EXP to J-AD and AGL2013-42537-R to J-JL-M from the Ministerio de Economia y Competitividad (MINECO, co-financed FEDER funds), Spain. MM was supported by the Erasmus Mundus Scholarship-ACTION 2 WELCOME program of the European Commission. Research in CRAG is supported in part by CERCA (Generalitat de Catalunya) and by "Severo Ochoa Programme for Centres of Excellence in R&D" 2016-2019 (SEV-2015-0533).Cordero, T.; Mohamed, M.; Lopez Moya, J.; Daros Arnau, JA. (2017). A Recombinant Potato virus Y Infectious Clone Tagged with the Rosea1 Visual Marker (PVY-Ros1) Facilitates the Analysis of Viral Infectivity and Allows the Production of Large Amounts of Anthocyanins in Plants. Frontiers in Microbiology. 8:1-11. https://doi.org/10.3389/fmicb.2017.00611S1118Abdel-Hafez, S. I. I., Nafady, N. A., Abdel-Rahim, I. R., Shaltout, A. M., Daròs, J.-A., & Mohamed, M. A. (2016). Assessment of protein silver nanoparticles toxicity against pathogenic Alternaria solani. 3 Biotech, 6(2). doi:10.1007/s13205-016-0515-6Allan, A. C., Hellens, R. P., & Laing, W. A. (2008). MYB transcription factors that colour our fruit. Trends in Plant Science, 13(3), 99-102. doi:10.1016/j.tplants.2007.11.012An, C. H., Lee, K.-W., Lee, S.-H., Jeong, Y. J., Woo, S. G., Chun, H., … Kim, C. Y. (2015). Heterologous expression of IbMYB1a by different promoters exhibits different patterns of anthocyanin accumulation in tobacco. Plant Physiology and Biochemistry, 89, 1-10. doi:10.1016/j.plaphy.2015.02.002Atreya, P. L., Lopez-Moya, J. J., Chu, M., Atreya, C. D., & Pirone, T. P. (1995). Mutational analysis of the coat protein N-terminal amino acids involved in potyvirus transmission by aphids. Journal of General Virology, 76(2), 265-270. doi:10.1099/0022-1317-76-2-265Baulcombe, D. C., Chapman, S., & Cruz, S. (1995). Jellyfish green fluorescent protein as a reporter for virus infections. The Plant Journal, 7(6), 1045-1053. doi:10.1046/j.1365-313x.1995.07061045.xBedoya, L., Martínez, F., Rubio, L., & Daròs, J.-A. (2010). Simultaneous equimolar expression of multiple proteins in plants from a disarmed potyvirus vector. Journal of Biotechnology, 150(2), 268-275. doi:10.1016/j.jbiotec.2010.08.006Bedoya, L. C., & Daròs, J.-A. (2010). Stability of Tobacco etch virus infectious clones in plasmid vectors. Virus Research, 149(2), 234-240. doi:10.1016/j.virusres.2010.02.004Bedoya, L. C., Martínez, F., Orzáez, D., & Daròs, J.-A. (2012). Visual Tracking of Plant Virus Infection and Movement Using a Reporter MYB Transcription Factor That Activates Anthocyanin Biosynthesis. Plant Physiology, 158(3), 1130-1138. doi:10.1104/pp.111.192922Boyer, J.-C., & Haenni, A.-L. (1994). Infectious Transcripts and cDNA Clones of RNA Viruses. Virology, 198(2), 415-426. doi:10.1006/viro.1994.1053Chalfie, M., Tu, Y., Euskirchen, G., Ward, W., & Prasher, D. (1994). Green fluorescent protein as a marker for gene expression. Science, 263(5148), 802-805. doi:10.1126/science.8303295Cordero, T., Cerdán, L., Carbonell, A., Katsarou, K., Kalantidis, K., & Daròs, J.-A. (2017). Dicer-Like 4 Is Involved in Restricting the Systemic Movement of Zucchini yellow mosaic virus in Nicotiana benthamiana. Molecular Plant-Microbe Interactions®, 30(1), 63-71. doi:10.1094/mpmi-11-16-0239-rDolja, V. V., McBride, H. J., & Carrington, J. C. (1992). Tagging of plant potyvirus replication and movement by insertion of beta-glucuronidase into the viral polyprotein. Proceedings of the National Academy of Sciences, 89(21), 10208-10212. doi:10.1073/pnas.89.21.10208Elbeshehy, E. K. F., Elazzazy, A. M., & Aggelis, G. (2015). Silver nanoparticles synthesis mediated by new isolates of Bacillus spp., nanoparticle characterization and their activity against Bean Yellow Mosaic Virus and human pathogens. Frontiers in Microbiology, 6. doi:10.3389/fmicb.2015.00453Engler, C., & Marillonnet, S. (2013). Golden Gate Cloning. Methods in Molecular Biology, 119-131. doi:10.1007/978-1-62703-764-8_9FRENCH, R., JANDA, M., & AHLQUIST, P. (1986). Bacterial Gene Inserted in an Engineered RNA Virus: Efficient Expression in Monocotyledonous Plant Cells. Science, 231(4743), 1294-1297. doi:10.1126/science.231.4743.1294Gibbs, A., & Ohshima, K. (2010). Potyviruses and the Digital Revolution. Annual Review of Phytopathology, 48(1), 205-223. doi:10.1146/annurev-phyto-073009-114404Johansen, I. E. (1996). Intron insertion facilitates amplification of cloned virus cDNA in Escherichia coli while biological activity is reestablished after transcription in vivo. Proceedings of the National Academy of Sciences, 93(22), 12400-12405. doi:10.1073/pnas.93.22.12400Joshi, R. L., Joshi, V., & Ow, D. W. (1990). BSMV genome mediated expression of a foreign gene in dicot and monocot plant cells. The EMBO Journal, 9(9), 2663-2669. doi:10.1002/j.1460-2075.1990.tb07451.xKarasev, A. V., & Gray, S. M. (2013). Continuous and Emerging Challenges of Potato virus Y in Potato. Annual Review of Phytopathology, 51(1), 571-586. doi:10.1146/annurev-phyto-082712-102332Kelloniemi, J., Mäkinen, K., & Valkonen, J. P. T. (2008). Three heterologous proteins simultaneously expressed from a chimeric potyvirus: Infectivity, stability and the correlation of genome and virion lengths. Virus Research, 135(2), 282-291. doi:10.1016/j.virusres.2008.04.006Krenz, B., Bronikowski, A., Lu, X., Ziebell, H., Thompson, J. R., & Perry, K. L. (2015). Visual monitoring of Cucumber mosaic virus infection in Nicotiana benthamiana following transmission by the aphid vector Myzus persicae. Journal of General Virology, 96(9), 2904-2912. doi:10.1099/vir.0.000185Lara, H. H., Ixtepan-Turrent, L., Garza Treviño, E. N., & Singh, D. K. (2011). Use of silver nanoparticles increased inhibition of cell-associated HIV-1 infection by neutralizing antibodies developed against HIV-1 envelope proteins. Journal of Nanobiotechnology, 9(1), 38. doi:10.1186/1477-3155-9-38López-Moya, J. J., & Garcı́a, J. A. (2000). Construction of a stable and highly infectious intron-containing cDNA clone of plum pox potyvirus and its use to infect plants by particle bombardment. Virus Research, 68(2), 99-107. doi:10.1016/s0168-1702(00)00161-1Majer, E., Llorente, B., Rodríguez-Concepción, M., & Daròs, J.-A. (2017). Rewiring carotenoid biosynthesis in plants using a viral vector. Scientific Reports, 7(1). doi:10.1038/srep41645Marillonnet, S., Thoeringer, C., Kandzia, R., Klimyuk, V., & Gleba, Y. (2005). Systemic Agrobacterium tumefaciens–mediated transfection of viral replicons for efficient transient expression in plants. Nature Biotechnology, 23(6), 718-723. doi:10.1038/nbt1094Mishra, S., & Singh, H. B. (2014). Biosynthesized silver nanoparticles as a nanoweapon against phytopathogens: exploring their scope and potential in agriculture. Applied Microbiology and Biotechnology, 99(3), 1097-1107. doi:10.1007/s00253-014-6296-0Nie, B., Singh, M., Sullivan, A., Singh, R. P., Xie, C., & Nie, X. (2011). Recognition and Molecular Discrimination of Severe and Mild PVYO Variants of Potato virus Y in Potato in New Brunswick, Canada. Plant Disease, 95(2), 113-119. doi:10.1094/pdis-04-10-0257Olspert, A., Chung, B. Y., Atkins, J. F., Carr, J. P., & Firth, A. E. (2015). Transcriptional slippage in the positive‐sense RNA virus family Potyviridae. EMBO reports, 16(8), 995-1004. doi:10.15252/embr.201540509Passeri, V., Koes, R., & Quattrocchio, F. M. (2016). New Challenges for the Design of High Value Plant Products: Stabilization of Anthocyanins in Plant Vacuoles. Frontiers in Plant Science, 7. doi:10.3389/fpls.2016.00153Quenouille, J., Vassilakos, N., & Moury, B. (2013). Potato virus Y: a major crop pathogen that has provided major insights into the evolution of viral pathogenicity. Molecular Plant Pathology, 14(5), 439-452. doi:10.1111/mpp.12024Revers, F., & García, J. A. (2015). Molecular Biology of Potyviruses. Advances in Virus Research, 101-199. doi:10.1016/bs.aivir.2014.11.006Rodamilans, B., Valli, A., Mingot, A., San León, D., Baulcombe, D., López-Moya, J. J., & García, J. A. (2015). RNA Polymerase Slippage as a Mechanism for the Production of Frameshift Gene Products in Plant Viruses of the Potyviridae Family. Journal of Virology, 89(13), 6965-6967. doi:10.1128/jvi.00337-15Rodriguez, E. A., Campbell, R. E., Lin, J. Y., Lin, M. Z., Miyawaki, A., Palmer, A. E., … Tsien, R. Y. (2017). The Growing and Glowing Toolbox of Fluorescent and Photoactive Proteins. Trends in Biochemical Sciences, 42(2), 111-129. doi:10.1016/j.tibs.2016.09.010Rupar, M., Faurez, F., Tribodet, M., Gutiérrez-Aguirre, I., Delaunay, A., Glais, L., … Ravnikar, M. (2015). Fluorescently Tagged Potato virus Y: A Versatile Tool for Functional Analysis of Plant-Virus Interactions. Molecular Plant-Microbe Interactions®, 28(7), 739-750. doi:10.1094/mpmi-07-14-0218-taSaxena, P., Hsieh, Y.-C., Alvarado, V. Y., Sainsbury, F., Saunders, K., Lomonossoff, G. P., & Scholthof, H. B. (2010). Improved foreign gene expression in plants using a virus-encoded suppressor of RNA silencing modified to be developmentally harmless. Plant Biotechnology Journal, 9(6), 703-712. doi:10.1111/j.1467-7652.2010.00574.xSCHOLTHOF, K.-B. G., ADKINS, S., CZOSNEK, H., PALUKAITIS, P., JACQUOT, E., HOHN, T., … FOSTER, G. D. (2011). Top 10 plant viruses in molecular plant pathology. Molecular Plant Pathology, 12(9), 938-954. doi:10.1111/j.1364-3703.2011.00752.xThole, V., Worland, B., Snape, J. W., & Vain, P. (2007). The pCLEAN Dual Binary Vector System for Agrobacterium-Mediated Plant Transformation. Plant Physiology, 145(4), 1211-1219. doi:10.1104/pp.107.108563Tilsner, J., & Oparka, K. J. (2010). Tracking the green invaders: advances in imaging virus infection in plants. Biochemical Journal, 430(1), 21-37. doi:10.1042/bj20100372Zhang, Y., Butelli, E., & Martin, C. (2014). Engineering anthocyanin biosynthesis in plants. Current Opinion in Plant Biology, 19, 81-90. doi:10.1016/j.pbi.2014.05.011Zhao, X., Yuan, Z., Fang, Y., Yin, Y., & Feng, L. (2012). Characterization and evaluation of major anthocyanins in pomegranate (Punica granatum L.) peel of different cultivars and their development phases. European Food Research and Technology, 236(1), 109-117. doi:10.1007/s00217-012-1869-

Elite households in viejo sangayaico: a late horizon and early colonial settlement in huancavelica (Peru)

Recientes excavaciones llevadas a cabo al interior de dos estructuras domésticas (E19 y E12) en Viejo Sangayaico B (Huancavelica, Perú) revelan como los habitantes de ambas estructuras poseyeron un estatus de élite asociado a la administración inca del asentamiento durante el Horizonte Tardío. Asimismo, diferencias en la calidad y cantidad de bienes europeos consumidos durante las primeras décadas de la Colonia re!ejan dos distintas estrategias políticas asumidas por ambos grupos con el objetivo de mantener su estatus de élite en un contexto de profundos y rápidos cambios.Recent excavations carried out inside two household structures (E19 and E12) in Viejo Sangayaico B (Huancavelica, Peru) reveal how the inhabitants of both structures possessed an elite status associated with the Inca administration of the settlement during the Late Horizon. Likewise, differences in the quality and quantity of European goods consumed during the early decades of the colonial period reflect two different political strategies assumed by both groups in order to maintain their elite status in a context of deep and rapid changes.Fil: Rodriguez Morales, Jorge. Universidad Nacional Mayor de San Marcos; PerúFil: Lane, Kevin John. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Filosofía y Letras. Instituto de Arqueología; ArgentinaFil: Huamán, Oliver. Universidad Nacional Mayor de San Marcos; PerúFil: Chauca, George. Universidad Nacional Mayor de San Marcos; PerúFil: Coll, Luis Vicente Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Saavedra 15. Instituto de las Culturas. Universidad de Buenos Aires. Instituto de las Culturas; ArgentinaFil: Beresford Jones, David. University of Cambridge; Reino UnidoFil: French, Charles Andrew Ivey. University of Cambridge; Reino Unid

Observational Evidence of S-web Source of the Slow Solar Wind

From 2022 March 18 to 21, NOAA Active Region (AR) 12967 was tracked simultaneously by Solar Orbiter at 0.35 au and Hinode/EIS at Earth. During this period, strong blueshifted plasma upflows were observed along a thin, dark corridor of open magnetic field originating at the AR’s leading polarity and continuing toward the southern extension of the northern polar coronal hole. A potential field source surface model shows large lateral expansion of the open magnetic field along the corridor. Squashing factor Q-maps of the large-scale topology further confirm super-radial expansion in support of the S-web theory for the slow wind. The thin corridor of upflows is identified as the source region of a slow solar wind stream characterized by ∼300 km s−1 velocities, low proton temperatures of ∼5 eV, extremely high density >100 cm−3, and a short interval of moderate Alfvénicity accompanied by switchback events. When the connectivity changes from the corridor to the eastern side of the AR, the in situ plasma parameters of the slow solar wind indicate a distinctly different source region. These observations provide strong evidence that the narrow open-field corridors, forming part of the S-web, produce some extreme properties in their associated solar wind streams