Charge Photoinjection in Intercalated and Covalently Bound [Re(CO)_(3)(dppz)(py)]^(+)–DNA Constructs Monitored by Time-Resolved Visible and Infrared Spectroscopy

The complex [Re(CO)_(3)(dppz)(py′-OR)]+ (dppz = dipyrido[3,2-a:2′,3′-c]phenazine; py′-OR = 4-functionalized pyridine) offers IR sensitivity and can oxidize DNA directly from the excited state, making it a promising probe for the study of DNA-mediated charge transport (CT). The behavior of several covalent and noncovalent Re–DNA constructs was monitored by time-resolved IR (TRIR) and UV/visible spectroscopies, as well as biochemical methods, confirming the long-range oxidation of DNA by the excited complex. Optical excitation of the complex leads to population of MLCT and at least two distinct intraligand states. Experimental observations that are consistent with charge injection from these excited states include similarity between long-time TRIR spectra and the reduced state spectrum observed by spectroelectrochemistry, the appearance of a guanine radical signal in TRIR spectra, and the eventual formation of permanent guanine oxidation products. The majority of reactivity occurs on the ultrafast time scale, although processes dependent on slower conformational motions of DNA, such as the accumulation of oxidative damage at guanine, are also observed. The ability to measure events on such disparate time scales, its superior selectivity in comparison to other spectroscopic techniques, and the ability to simultaneously monitor carbonyl ligand and DNA IR absorption bands make TRIR a valuable tool for the study of CT in DNA

Pulsed negative pressure myofascial vacuum therapy and percutaneous electrolysis in the treatment of lateral epicondylalgia: A single-blind randomized controlled trial

Background Lateral Epicondylalgia (LE) represents one of the most common injuries of the upper limb. It is necessary to find effective treatments that reduce pain and increase functionality. Purpose To determine the effects of an integrated intervention of Pulsed Negative Pressure Myofascial Vacuum Therapy (VT), Percutaneous Electrolysis (PE) and eccentric exercise (EE) in the treatment of LE compared versus Manual Therapy soft tissue mobilization (MT) and Ultrasound therapy (US) and EE. Study design Single-blind randomized controlled trial. Methods Forty participants, with unilateral LE, were randomly divided into two groups: VT + PE + EE group (n = 20) and MT + US + EE group (n = 20). The VT + PE + EE group received one weekly session for four weeks and a regimen of EE daily at-home, and the MT + US + EE group received 10 sessions over a period of two weeks and a regimen of EE daily at-home. Numerical pain rating scale (NPRS), range of motion (ROM) pressure pain threshold (PPT) and function (PRTEE questionnaire) were measured before treatment, at the end of treatment, and at one- and three-month follow-ups. Results The statistically significant improvements were found post-treatment, favoring the VT + PE group in pain intensity (p < 0.001; ES = 0.408), PRTEE-S Pain (p = 0.001; ES = 0.377), PRTEE-S Specific function (p = 0.004; ES = 0.306) and PRTEE-S Total (p = 0.001; ES = 0.355). The VT + PE + EE treatment showed greater effectiveness than the MT + US + EE treatment at immediate post-treatment, as well as at the one-month and three-months follow-up. Conclusions VT and PE added to an EE program could be an effective treatment for pain, ROM, PPT, and function in patients with LE. List of abbreviations ICCIntraclass Correlation CoefficientLELateral EpicondylalgiaPEPercutaneous ElectrolysisMDCMinimum Detectable ChangeMTManual Therapy soft tissue mobilizationNPRSNumerical pain rating scalePPTpressure pain thresholdPRTEE questionnairePatient-Rated Tennis Elbow Evaluation QuestionnaireROMrange of motionSEMStandard Error of MeasurementVTPulsed Negative Pressure Myofascial Vacuum TherapyUSUltrasound therap

Synthesis, Photochemical, and Redox Properties of Gold(I) and Gold(III) Pincer Complexes Incorporating a 2,2′:6′,2″-Terpyridine Ligand Framework

Reaction of [Au(C6F5)(tht)] (tht = tetrahydrothiophene) with 2,2′:6′,2″-terpyridine (terpy) leads to complex [Au(C6F5)(η1-terpy)] (1). The chemical oxidation of complex (1) with 2 equiv of [N(C6H4Br-4)3](PF6) or using electrosynthetic techniques affords the Au(III) complex [Au(C6F5)(η3-terpy)](PF6)2 (2). The X-ray diffraction study of complex 2 reveals that the terpyridine acts as tridentate chelate ligand, which leads to a slightly distorted square-planar geometry. Complex 1 displays fluorescence in the solid state at 77 K due to a metal (gold) to ligand (terpy) charge transfer transition, whereas complex 2 displays fluorescence in acetonitrile due to excimer or exciplex formation. Time-dependent density functional theory calculations match the experimental absorption spectra of the synthesized complexes. In order to further probe the frontier orbitals of both complexes and study their redox behavior, each compound was separately characterized using cyclic voltammetry. The bulk electrolysis of a solution of complex 1 was analyzed by spectroscopic methods confirming the electrochemical synthesis of complex 2

Estudio comparativo sobre estimulación temprana entre el método Montessori y el Tradicional, en niños de dos años y medio a tres años

Trabajo final de graduación compuesto de 91 páginas en formato PDF.El presente estudio comparativo nace al considerar la importancia que tiene la estimulación temprana en los niños(as), siendo un tema de gran interés no sólo para los padres sino para las diferentes instituciones encargadas del cuido y de la enseñanza y el aprendizaje de los niños(as). El objetivo de esta investigación es comparar el desempeño de la estimulación temprana que ofrece el método Montessori, con la que ofrece el tradicional, en las diferentes áreas de desarrollo, (cognitivo, psicomotor, socio-afectivo y lenguaje oral). También interesa valorar la necesidad de la estimulación temprana e identificar el grado de desarrollo de los niños(as) de dichas instituciones. Otro factor de interés en el estudio es determinar la opinión de las docentes de cada grupo, sobre la efectividad del método que utilizan y las actividades que realizan para la estimulación temprana. El método Montessori plantea que el niño(a), adquiere el aprendizaje por medio del juego y que éste debe explorar su entorno, en un ambiente controlado mediante la observación y la supervisión de los profesionales a cargo (guías). Los principios del método están basados en el amor y el respeto como pilares para la enseñanza aprendizaje, destacando la capacidad que tienen los niños (as) de lograr aprender de forma independiente.Universidad Estatal a Distancia de Costa Ric

Risk Analysis of Acute and Chronic Exposure to Arsenic of the Inhabitants in a District of Buenos Aires, Argentina

The arsenic occurrence in the water constitutes a serious world health concern due to its toxicity. Depending on the intensity and duration of exposure, this element can be acutely lethal or may have a wide range of health effects in humans and animals. In Argentina, the origin of arsenic is mainly natural, and related to different geological processes. The Argentinean concern about arsenic and its influence on human health dates back to the previous century. The disease ascribed to arsenic contamination was called ‘chronic regional endemic hydroarsenism’. It is produced by the consumption of water with high levels of this element. In our study, we focused in La Matanza district, a very populated site in the Buenos Aires Province. An increasing concern of the inhabitants of the area regarding health problems was detected. In order to establish a full view of arsenic exposure in the area, several matrices and targets were analyzed. As matrices, water and soil samples were analyzed. As targets, canine and human hair was studied. The aim of this study was to investigate acute and chronic exposure to arsenic of La Matanza inhabitants

Shallow buried SiN x layers

Copyright (2000) American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics. The following article appeared in Journal of Applied Physics 87.1 (2000): 8201-8203 and may be found at http://jap.aip.org

Exploring the Taxonomical and Functional Profile of As Burgas Hot Spring Focusing On Thermostable β-galactosidases

[Abstract] In the present study we investigate the microbial community inhabiting As Burgas geothermal spring, located in Ourense (Galicia, Spain). The approximately 23 Gbp of Illumina sequences generated for each replicate revealed a complex microbial community dominated by Bacteria in which Proteobacteria and Aquificae were the two prevalent phyla. An association between the two most prevalent genera, Thermus and Hydrogenobacter, was suggested by the relationship of their metabolism. The high relative abundance of sequences involved in the Calvin–Benson cycle and the reductive TCA cycle unveils the dominance of an autotrophic population. Important pathways from the nitrogen and sulfur cycle are potentially taking place in As Burgas hot spring. In the assembled reads, two complete ORFs matching GH2 beta-galactosidases were found. To assess their functional characterization, the two ORFs were cloned and overexpressed in E. coli. The pTsbg enzyme had activity towards o-Nitrophenyl-β-D-galactopyranoside (ONPG) and p-Nitrophenyl-β-D-fucopyranoside, with high thermal stability and showing maximal activity at 85 °C and pH 6, nevertheless the enzyme failed to hydrolyze lactose. The other enzyme, Tsbg, was unable to hydrolyze even ONPG or lactose. This finding highlights the challenge of finding novel active enzymes based only on their sequence.Xunta de Galicia; ED431C 2020/08Ministerio de Ciencia, Innovación y Universidades; RTI2018-099249-B-I00Ministerio de Educación Cultura y Deporte; FPU12/0505

Application of receiver operating characteristic (ROC) methodology in biological studies on marine resources: sex determination of Paracentrotus lividus (Lamarck, 1816)

The receiver operating characteristic (ROC) curve is usually used in biomedicine as an indicator of the accuracy of diagnostic tests. However, this measure of discrimination has been little used in other areas, such as animal biology or ecology. We present a novel application of an ROC analysis in which gonad colour was used to determine the sex of Paracentrotus lividus (Lamarck, 1816), a sea urchin of considerable commercial interest. A better classifier than gonad colour was obtained by transforming these colours through flexible logistic generalized additive models.Peer Reviewe

Roman mining works and gold ore deposits in the San Pedro de Olleros sector (El Bierzo, León)

[Abstract] The gold mining in the Bierzo Region, NW Spain had great importance, not only in the well known Las Médulas site, but also in the north sector of El Bierzo Basin, (San Pedro de Olleros Valle de Finolledo). This work have recognised the roman exploitation sites, differentiating the fertile facies within the tertiary sequences. Although no data about ages have still found, an approach to the tectonosedimentary evolutive history is carried out, taking into account the geometry and characteristics of the deposits[Abstract] The gold mining in the Bierzo Region, NW Spain had great importance, not only in the well known Las Médulas site, but also in the north sector of El Bierzo Basin, (San Pedro de Olleros -Valle de Finolledo). This work have recognised the roman exploitation sites, differentiating the fertile facies within the tertiary sequences. Although no data about ages have still found, an approach to the tectonosedimentary evolutive history is carried out, taking into account the geometry and characteristics of the deposit