Association of Growth Substrates and Bacterial Genera with Benzo[ a ]pyrene Mineralization in Contaminated Soil

Benzo[a]pyrene (BaP) is a carcinogenic polycyclic aromatic hydrocarbon (PAH) that is not known to be a bacterial growth substrate. Organisms capable of cometabolizing BaP in complex field-contaminated systems have not previously been identified. We evaluated BaP mineralization by a bacterial community from a bioreactor treating PAH-contaminated soil during coincubation with or after pre-enrichment on various PAHs as growth substrates. Pyrosequence libraries of 16S rRNA genes were used to identify bacteria that were enriched on the added growth substrate as a means of associating specific organisms with BaP mineralization. Coincubating the bioreactor-treated soil with naphthalene, phenanthrene, or pyrene inhibited BaP mineralization, whereas pre-enriching the soil on the same three PAHs enhanced BaP mineralization. Combined, these results suggest that bacteria in the bioreactor community that are capable of growing on naphthalene, phenanthrene, and/or pyrene can metabolize BaP, with coincubation competitively inhibiting BaP metabolism. Anthracene, fluoranthene, and benz[a]anthracene had little effect on BaP mineralization compared to incubations without an added growth substrate under either coincubation or pre-enrichment conditions. Substantial increases in relative abundance after pre-enrichment with phenanthrene, naphthalene, or pyrene, but not the other PAHs, suggest that members of the genera Cupriavidus and Luteimonas may have been associated with BaP mineralization

Identification of Anthraquinone-Degrading Bacteria in Soil Contaminated with Polycyclic Aromatic Hydrocarbons

ABSTRACT Quinones and other oxygenated polycyclic aromatic hydrocarbons (oxy-PAHs) are toxic and/or genotoxic compounds observed to be cocontaminants at PAH-contaminated sites, but their formation and fate in contaminated environmental systems have not been well studied. Anthracene-9,10-dione (anthraquinone) has been found in most PAH-contaminated soils and sediments that have been analyzed for oxy-PAHs. However, little is known about the biodegradation of oxy-PAHs, and no bacterial isolates have been described that are capable of growing on or degrading anthraquinone. PAH-degrading Mycobacterium spp. are the only organisms that have been investigated to date for metabolism of a PAH quinone, 4,5-pyrenequinone. We utilized DNA-based stable-isotope probing (SIP) with [U- 13 C]anthraquinone to identify bacteria associated with anthraquinone degradation in PAH-contaminated soil from a former manufactured-gas plant site both before and after treatment in a laboratory-scale bioreactor. SIP with [U- 13 C]anthracene was also performed to assess whether bacteria capable of growing on anthracene are the same as those identified to grow on anthraquinone. Organisms closely related to Sphingomonas were the most predominant among the organisms associated with anthraquinone degradation in bioreactor-treated soil, while organisms in the genus Phenylobacterium comprised the majority of anthraquinone degraders in the untreated soil. Bacteria associated with anthracene degradation differed from those responsible for anthraquinone degradation. These results suggest that Sphingomonas and Phenylobacterium species are associated with anthraquinone degradation and that anthracene-degrading organisms may not possess mechanisms to grow on anthraquinone

IHD-Cebrap project on Labour Market Inequality in Brazil and India

In segmented labour markets like those of Brazil and India, vocational training policies tend to reinforce labour market inequality. This review looks at VET policies and institutions in order to understand its potential as a tool for inequality reduction in the labour market. As skills are an important determinant of position in a hierarchy of jobs, they act as a basis for differentiating the workforce and thus are linked to labour market inequality. While the data for the two countries are not directly comparable, it is clear that in both Brazil and India formal VET is concentrated among the better educated

The Physiological Mechanisms of Effect of Vitamins and Amino Acids on Tendon and Muscle Healing: A Systematic Review

© 2018 Human Kinetics, Inc.To evaluate the current literature via systematic review to ascertain whether amino acids/vitamins provide any influence on musculotendinous healing and if so, by which physiological mechanisms. Methods: EBSCO, PubMed, ScienceDirect, Embase Classic/Embase, and MEDLINE were searched using terms including "vitamins," "amino acids," "healing," "muscle," and "tendon." The primary search had 479 citations, of which 466 were excluded predominantly due to nonrandomized design. Randomized human and animal studies investigating all supplement types/forms of administration were included. Critical appraisal of internal validity was assessed using the Cochrane risk of Bias Tool or the Systematic Review Centre for Laboratory Animal Experimentation Risk of Bias Tool for human and animal studies, respectively. Two reviewers performed duel data extraction. Results: Twelve studies met criteria for inclusion: eight examined tendon healing and four examined muscle healing. All studies used animal models, except two human trials using a combined integrator. Narrative synthesis was performed via content analysis of demonstrated statistically significant effects and thematic analysis of proposed physiological mechanisms of intervention. Vitamin C/taurine demonstrated indirect effects on tendon healing through antioxidant activity. Vitamin A/glycine showed direct effects on extracellular matrix tissue synthesis. Vitamin E shows an antiproliferative influence on collagen deposition. Leucine directly influences signaling pathways to promote muscle protein synthesis. Discussion: Preliminary evidence exists, demonstrating that vitamins and amino acids may facilitate multilevel changes in musculotendinous healing; however, recommendations on clinical utility should be made with caution. All animal studies and one human study showed high risk of bias with moderate interobserver agreement (k = 0.46). Currently, there is limited evidence to support the use of vitamins and amino acids for musculotendinous injury. Both high-quality animal experimentation of the proposed mechanisms confirming the physiological influence of supplementation and human studies evaluating effects on tissue morphology and biochemistry are required before practical application.Peer reviewe

Optical spectroscopic variability of Herbig Ae/Be stars

We analysed 337 multi-epoch optical spectra of 38 Herbig Ae/Be (HAeBe) stars to gain insights into the variability behaviour of the circumstellar (CS) atomic gas. Equivalent widths (EWs) and line fluxes of the Halpha, [OI]6300, HeI5876 and NaID lines were obtained for each spectrum; the Halpha line width at 10% of peak intensity (W10) and profile shapes were also measured and classified. The mean line strengths and relative variabilities were quantified for each star. Simultaneous optical photometry was used to estimate the line fluxes. We present a homogeneous spectroscopic database of HAeBe stars. The lines are variable in practically all stars and timescales, although 30 % of the objects show a constant EW in [OI]6300, which is also the only line that shows no variability on timescales of hours. The HeI5876 and NaID EW relative variabilities are typically the largest, followed by those in [OI]6300 and Halpha. The EW changes can be larger than one order of magnitude for the HeI5876 line, and up to a factor 4 for Halpha. The [OI]6300 and Halpha EW relative variabilities are correlated for most stars in the sample. The Halpha mean EW and W10 are uncorrelated, as are their relative variabilities. The Halpha profile changes in 70 % of the objects. The massive stars in the sample usually show more stable Halpha profiles with blueshifted self-absorptions and less variable 10% widths. Our data suggest multiple causes for the different line variations, but the [OI]6300 and Halpha variability must share a similar origin in many objects. The physical mechanism responsible for the Halpha line broadening does not depend on the amount of emission; unlike in lower-mass stars, physical properties based on the Halpha luminosity and W10 would significantly differ. Our results provide additional support to previous works that reported different physical mechanisms in Herbig Ae and Herbig Be stars.Comment: 10 pages, 5 figures, 2 appendixe

The mass ratio and formation mechanisms of Herbig Ae/Be star binary systems

We present B and R band spectroastrometry of a sample of 45 Herbig Ae/Be stars in order to study their binary properties. All but one of the targets known to be binary systems with a separation of ~0.1-2.0 arcsec are detected by a distinctive spectroastrometric signature. Some objects in the sample exhibit spectroastrometric features that do not appear attributable to a binary system. We find that these may be due to light reflected from dusty halos or material entrained in winds. We present 8 new binary detections and 4 detections of an unknown component in previously discovered binary systems. The data confirm previous reports that Herbig Ae/Be stars have a high binary fraction, 74+/-6 per cent in the sample presented here. We use a spectroastrometric deconvolution technique to separate the spatially unresolved binary spectra into the individual constituent spectra. The separated spectra allow us to ascertain the spectral type of the individual binary components, which in turn allows the mass ratio of these systems to be determined. In addition, we appraise the method used and the effects of contaminant sources of flux. We find that the distribution of system mass ratios is inconsistent with random pairing from the Initial Mass Function, and that this appears robust despite a detection bias. Instead, the mass ratio distribution is broadly consistent with the scenario of binary formation via disk fragmentation.Comment: Accepted for publication in MNRAS, minor changes made in proof stag

Cryptochrome 1 in Retinal Cone Photoreceptors Suggests a Novel Functional Role in Mammals

Cryptochromes are a ubiquitous group of blue-light absorbing flavoproteins that in the mammalian retina have an important role in the circadian clock. In birds, cryptochrome 1a (Cry1a), localized in the UV/violet-sensitive S1 cone photoreceptors, is proposed to be the retinal receptor molecule of the light-dependent magnetic compass. The retinal localization of mammalian Cry1, homologue to avian Cry1a, is unknown, and it is open whether mammalian Cry1 is also involved in magnetic field sensing. To constrain the possible role of retinal Cry1, we immunohistochemically analysed 90 mammalian species across 48 families in 16 orders, using an antiserum against the Cry1 C-terminus that in birds labels only the photo-activated conformation. In the Carnivora families Canidae, Mustelidae and Ursidae, and in some Primates, Cry1 was consistently labeled in the outer segment of the shortwave-sensitive S1 cones. This finding would be compatible with a magnetoreceptive function of Cry1 in these taxa. In all other taxa, Cry1 was not detected by the antiserum that likely also in mammals labels the photo-activated conformation, although Western blots showed Cry1 in mouse retinal cell nuclei. We speculate that in the mouse and the other negative-tested mammals Cry1 is involved in circadian functions as a non-light-responsive protein