Laboratory Animal Science AD 2000

The writer attempts to predict events likely to affect the future practice of Laboratory Animal Science. It is expected the number of experimental animals used will decline over the next decades for several reasons. These include a greater emphasis in research at the cellular, rather than the whole body, level and the redirection of much of the present animal based research of human diseases to man himself as the main experimental subject. This last prediction is based onan assessment of developments in technology and socio-economic factors whereby knowledge and control of human diseases will be obtained by epidemiological and non-invasive studies of human populations.The longterrn effects of the economic recession which have affected considerably animal based and other research in most countries, are also considered, as too is the impact of new legislation in some and, overall, the changing attitude of scientists, technicians and the general public on the experimental use of animals.The consequences of the decline in the number of experimental animals used, the availability of better experimental animals commercially, the recognition of the need for better experimental models and design upon the management of animal houses and the employment of animal technicians is then assessed.It is concluded that economic and scientific factors will lead to new concepts of animal house design and function. Of critical importance, it is considered, is recognition that the animal technician of the future should be selected, trained and qualified to fulfil an exacting scientific role if Laboratory Animal Science is to be effectively, economically and humanely practised

Animal house costs and management

In this paper, a sequel to 'Animal House Costs' (Dalton 1989), the author describes aspects of management of a large University animal house, making economies in annual expenditure and improving efficiency. Relative costs of ’in-house’ breeding and purchasing animals are considered and the problems of adapting animal house accomodation to meet changing requirements. Some fundamental concepts of animal accommodation are questioned and how these may relate to making savings on building capital and recurrent costs

Normalised Root Mean Square and Amplitude of Sidebands of Vibration Response as Tools for Gearbox Diagnosis

Quick assessment of the condition of gearboxes used in helicopters is a safety requirement. One of the most widely used helicopter on-board-mounted condition monitoring system these days is the Health and Usage Monitoring System. It has been specifically designed to monitor the condition of all safety-critical components operating in the helicopter through calculation of so-called condition indicators (CIs) - signal processing routines designed to output a single number that represents the condition of the monitored component. Among number of available parameters, there is a couple of CIs that over the years of testing have earned a reputation of being the most reliable measures of the gear tooth condition. At the same time, however, it has been observed that in some cases, those techniques do not properly indicate the deteriorating condition with the propagation of a gear tooth fault with the period of operation. Hence, three more robust methods have been suggested, which are discussed in this article

Expand+Functional selection and systematic analysis of intronic splicing elements identify active sequence motifs and associated splicing factors

Despite the critical role of pre-mRNA splicing in generating proteomic diversity and regulating gene expression, the sequence composition and function of intronic splicing regulatory elements (ISREs) have not been well elucidated. Here, we employed a high-throughput in vivo Screening PLatform for Intronic Control Elements (SPLICE) to identify 125 unique ISRE sequences from a random nucleotide library in human cells. Bioinformatic analyses reveal consensus motifs that resemble splicing regulatory elements and binding sites for characterized splicing factors and that are enriched in the introns of naturally occurring spliced genes, supporting their biological relevance. In vivo characterization, including an RNAi silencing study, demonstrate that ISRE sequences can exhibit combinatorial regulatory activity and that multiple trans-acting factors are involved in the regulatory effect of a single ISRE. Our work provides an initial examination into the sequence characteristics and function of ISREs, providing an important contribution to the splicing code

Accounting for Exchange Transactions: An Alternative Perspective

This paper presents a simple alternative method of recording exchange transactions that is theoretically superior to the popular gross method and is more informative than either the net or gross method

Blocking neutrophil integrin activation prevents ischemia-reperfusion injury.

Neutrophil recruitment, mediated by β2 integrins, combats pyogenic infections but also plays a key role in ischemia-reperfusion injury and other inflammatory disorders. Talin induces allosteric rearrangements in integrins that increase affinity for ligands (activation). Talin also links integrins to actin and other proteins that enable formation of adhesions. Structural studies have identified a talin1 mutant (L325R) that perturbs activation without impairing talin's capacity to link integrins to actin and other proteins. Here, we found that mice engineered to express only talin1(L325R) in myeloid cells were protected from renal ischemia-reperfusion injury. Dissection of neutrophil function in vitro and in vivo revealed that talin1(L325R) neutrophils had markedly impaired chemokine-induced, β2 integrin-mediated arrest, spreading, and migration. Surprisingly, talin1(L325R) neutrophils exhibited normal selectin-induced, β2 integrin-mediated slow rolling, in sharp contrast to the defective slow rolling of neutrophils lacking talin1 or expressing a talin1 mutant (W359A) that blocks talin interaction with integrins. These studies reveal the importance of talin-mediated activation of integrins for renal ischemia-reperfusion injury. They further show that neutrophil arrest requires talin recruitment to and activation of integrins. However, although neutrophil slow rolling requires talin recruitment to integrins, talin-mediated integrin activation is dispensable

Gender Differences in Cooperative Learning with University Students

Differences in achievement were investigated for 80 female and 80 male university students who were randomly assigned to either cooperative or competitive teaching methods. After viewing a videotaped instruction on research design, participants completed a mini-assignment either individually in the competitive condition or with a same-sex partner in the cooperative condition. All participants individually completed a multiple-choice test to assess achievement. Although no differences were found on the multiple-choice test, on the mini-assignment women scored significantly higher in the cooperative than in the competitive condition, whereas men performed about equally in both conditions.Nous avons étudié les différences de performance chez 80 étudiants et 80 étudiantes universitaires à qui l’on avait assigné, par hasard, un projet conforme, soit à des méthodes de coopération, soit à des méthodes concurrentielles. Après avoir visionné une vidéo de directives portant sur la méthodologie de recherche, les participants ont complété un petit travail seul, dans un contexte concurrentiel, ou bien avec un partenaire de même sexe dans un contexte de coopération. De plus, tous les participants ont complété un examen à choix multiples individuellement, pour mesurer leur performance. Les résultats de cet examen ne révèlent aucune différence. Toutefois, la note e qu’ont obtenue les femmes pour le petit travail était bien plus élevée pour celui accompli dans un contexte de coopération que dans un contexte de concurrence. La performance des hommes était semblable dans les deux contextes