The Vehicle, Spring 1980

Vol. 3, No. 2 Table of Contents White LanguageStarla Stensaaspage 4 The Grand Canyon at SunsetElisabeth Cristpage 5 WitheringSheila Kattypage 5 In Defense of the DandelionKathleen Alakspage 6 HaikuScott Fishelpage 7 Double SolitaireCathy Georgepage 8 AdolescenceNancy Douglaspage 9 Sunrises as SunsetsRobert Schumacherpage 9 War Is Not All HellChris Goerlichpage 11 Young SoldierRobert Swansonpage 11 Without Really Looking For ThemSheila Kattypage 12 JourneyJean Wallacepage 15 I Barely Remember the Poet/TeacherStarla Stensaaspage 16 To SearchKaren Buchananpage 17 Four-Hundred and Twenty-Four DaysAnnette Heinzpage 19 Killing CarpJerry McAnultypage 20 War of the WillsLaurel Anzelmopage 21 Life of a Sexual MetaphorChris Goerlichpage 23 Hazy Days in Mid-JulyKaren Buchananpage 24 August\u27s EndCynthia Rozminpage 24 Visiting GrandmotherRobert Swansonpage 26 UntitledNancy Van Cleavepage 27 The AlbinoDenise Davinroypage 27 This ChristmasRobert Schumacherpage 28 Burnt OfferingGinny Stroheckerpage 29 Long-term VacationDenise Davinroypage 29 Storm\u27s EveJeffrey Stousepage 30 Lovers: A Weaver in the StatesStarla Stensaaspage 31 Gitchegumme BeachScott Fishelpage 32 Dear Husband, What Do You Think?Katherine Adamspage 33 Cat\u27s Eve InnCynthia Rozminpage 34 BassScott Fishelpage 34 Fall Morning in the Okanagan ValleyJerry McAnultypage 35 MusicMary McDanielpage 36 AnticipationNancy Douglaspage 41 Don\u27t Let Your Bread LoafJohn Stockmanpage 41 The FieldElise Hempelpage 42 the g.a.Carla Vitezpage 42 Quality in Search of An AuthorRobert Schinaglpage 43 February 9thLisa Livingstonpage 44 By A Pond in SpringJohn Stockmanpage 46 Night SoundsJeffrey Stousepage 47 Art CoverKaren Dankovich PhotographCindy Hubbarttpage 3 PhotographScott Fishelpage 10 PhotographLisa Larsonpage 18 EtchingKaren Dankovichpage 25 PhotographScott Fishelpage 30 PhotographScott Fishelpage 40 PhotographCindy Hubbarttpage 45 PhotographCindy Hubbarttpage 48https://thekeep.eiu.edu/vehicle/1037/thumbnail.jp

The Vehicle, Spring 1980

Vol. 3, No. 2 Table of Contents White LanguageStarla Stensaaspage 4 The Grand Canyon at SunsetElisabeth Cristpage 5 WitheringSheila Kattypage 5 In Defense of the DandelionKathleen Alakspage 6 HaikuScott Fishelpage 7 Double SolitaireCathy Georgepage 8 AdolescenceNancy Douglaspage 9 Sunrises as SunsetsRobert Schumacherpage 9 War Is Not All HellChris Goerlichpage 11 Young SoldierRobert Swansonpage 11 Without Really Looking For ThemSheila Kattypage 12 JourneyJean Wallacepage 15 I Barely Remember the Poet/TeacherStarla Stensaaspage 16 To SearchKaren Buchananpage 17 Four-Hundred and Twenty-Four DaysAnnette Heinzpage 19 Killing CarpJerry McAnultypage 20 War of the WillsLaurel Anzelmopage 21 Life of a Sexual MetaphorChris Goerlichpage 23 Hazy Days in Mid-JulyKaren Buchananpage 24 August\u27s EndCynthia Rozminpage 24 Visiting GrandmotherRobert Swansonpage 26 UntitledNancy Van Cleavepage 27 The AlbinoDenise Davinroypage 27 This ChristmasRobert Schumacherpage 28 Burnt OfferingGinny Stroheckerpage 29 Long-term VacationDenise Davinroypage 29 Storm\u27s EveJeffrey Stousepage 30 Lovers: A Weaver in the StatesStarla Stensaaspage 31 Gitchegumme BeachScott Fishelpage 32 Dear Husband, What Do You Think?Katherine Adamspage 33 Cat\u27s Eve InnCynthia Rozminpage 34 BassScott Fishelpage 34 Fall Morning in the Okanagan ValleyJerry McAnultypage 35 MusicMary McDanielpage 36 AnticipationNancy Douglaspage 41 Don\u27t Let Your Bread LoafJohn Stockmanpage 41 The FieldElise Hempelpage 42 the g.a.Carla Vitezpage 42 Quality in Search of An AuthorRobert Schinaglpage 43 February 9thLisa Livingstonpage 44 By A Pond in SpringJohn Stockmanpage 46 Night SoundsJeffrey Stousepage 47 Art CoverKaren Dankovich PhotographCindy Hubbarttpage 3 PhotographScott Fishelpage 10 PhotographLisa Larsonpage 18 EtchingKaren Dankovichpage 25 PhotographScott Fishelpage 30 PhotographScott Fishelpage 40 PhotographCindy Hubbarttpage 45 PhotographCindy Hubbarttpage 48https://thekeep.eiu.edu/vehicle/1037/thumbnail.jp

Factors Associated with Revision Surgery after Internal Fixation of Hip Fractures

Background: Femoral neck fractures are associated with high rates of revision surgery after management with internal fixation. Using data from the Fixation using Alternative Implants for the Treatment of Hip fractures (FAITH) trial evaluating methods of internal fixation in patients with femoral neck fractures, we investigated associations between baseline and surgical factors and the need for revision surgery to promote healing, relieve pain, treat infection or improve function over 24 months postsurgery. Additionally, we investigated factors associated with (1) hardware removal and (2) implant exchange from cancellous screws (CS) or sliding hip screw (SHS) to total hip arthroplasty, hemiarthroplasty, or another internal fixation device. Methods: We identified 15 potential factors a priori that may be associated with revision surgery, 7 with hardware removal, and 14 with implant exchange. We used multivariable Cox proportional hazards analyses in our investigation. Results: Factors associated with increased risk of revision surgery included: female sex, [hazard ratio (HR) 1.79, 95% confidence interval (CI) 1.25-2.50; P = 0.001], higher body mass index (fo

Plakoglobin deficiency protects keratinocytes from apoptosis

The armadillo family protein plakoglobin (Pg) is a well-characterized component of anchoring junctions, where it functions to mediate cell-cell adhesion and maintain epithelial tissue integrity. Although its closest homolog beta-catenin acts in the Wnt signaling pathway to dictate cell fate and promote proliferation and survival, the role of Pg in these processes is not well understood. Here, we investigate how Pg affects the survival of mouse keratinocytes by challenging both Pg-null cells and their heterozygote counterparts with apoptotic stimuli. Our results indicate that Pg deletion protects keratinocytes from apoptosis, with null cells exhibiting delayed mitochondrial cytochrome c release and activation of caspase-3. Pg-null keratinocytes also exhibit increased messenger RNA and protein levels of the anti-apoptotic molecule Bcl-X(L) compared to heterozygote controls. Importantly, reintroduction of Pg into the null cells shifts their phenotype towards that of the Pg+/- keratinocytes, providing further evidence that Pg plays a direct role in regulating cell survival. Taken together, our results suggest that in addition to its adhesive role in epithelia, Pg may also function in contrast to the pro-survival tendencies of beta-catenin, to potentiate death in cells damaged by apoptotic stimuli, perhaps limiting the potential for the propagation of mutations and cellular transformation.Journal of Investigative Dermatology advance online publication, 16 November 2006; doi:10.1038/sj.jid.5700615

Activated Ras requires autophagy to maintain oxidative metabolism and tumorigenesis

Autophagy is a catabolic pathway used by cells to support metabolism in response to starvation and to clear damaged proteins and organelles in response to stress. We report here that expression of a H-rasV12 or K-rasV12 oncogene up-regulates basal autophagy, which is required for tumor cell survival in starvation and in tumorigenesis. In Ras-expressing cells, defective autophagosome formation or cargo delivery causes accumulation of abnormal mitochondria and reduced oxygen consumption. Autophagy defects also lead to tricarboxylic acid (TCA) cycle metabolite and energy depletion in starvation. As mitochondria sustain viability of Ras-expressing cells in starvation, autophagy is required to maintain the pool of functional mitochondria necessary to support growth of Ras-driven tumors. Human cancer cell lines bearing activating mutations in Ras commonly have high levels of basal autophagy, and, in a subset of these, down-regulating the expression of essential autophagy proteins impaired cell growth. As cancers with Ras mutations have a poor prognosis, this “autophagy addiction” suggests that targeting autophagy and mitochondrial metabolism are valuable new approaches to treat these aggressive cancers

Transducin β-like protein 1 controls multiple oncogenic networks in diffuse large B-cell lymphoma.

Diffuse large B-cell lymphoma (DLBCL) is the most common Non-Hodgkin\u27s lymphoma and is characterized by a remarkable heterogeneity with diverse variants that can be identified histologically and molecularly. Large-scale gene expression profiling studies have identified the germinal center B-cell (GCB-) and activated B-cell (ABC-) subtypes. Standard chemo-immunotherapy remains standard front line therapy, curing approximately two thirds of patients. Patients with refractory disease or those who relapse after salvage treatment have an overall poor prognosis highlighting the need for novel therapeutic strategies. Transducin β-like protein 1 (TBL1) is an exchange adaptor protein encoded by the TBL1X gene and known to function as a master regulator of the Wnt signalling pathway by binding to β-CATENIN and promoting its downstream transcriptional program. Here, we show that, unlike normal B-cells, DLBCL cells express abundant levels of TBL1 and its overexpression correlates with poor clinical outcome regardless of DLBCL molecular subtype. Genetic deletion of TBL1 and pharmacological approach using tegavivint, a first-in-class small molecule targeting TBL1 (Iterion Therapeutics), promotes DLBCL cell death in vitro and in vivo. Through an integrated genomic, biochemical, and pharmacologic analyses, we characterized a novel, β-CATENIN independent, post-transcriptional oncogenic function of TBL1 in DLBCL where TBL1 modulates the stability of key oncogenic proteins such as PLK1, MYC, and the autophagy regulatory protein BECLIN-1 through its interaction with a SKP1-CUL1-F-box (SCF) protein supercomplex. Collectively, our data provide the rationale for targeting TBL1 as a novel therapeutic strategy in DLBCL