Pushing the room temperature continous-wave operation limit of GaSb-based interband cascade lasers beyond 6 μ\mum

We present GaSb-based interband cascade lasers emitting at a center wavelength of 6.12 $\mu$m at 20$^\circ$C in continuous-wave operation up to a maximum operating temperature of 40$^\circ$C. Pulsed measurements based on broad area devices show improved performance by applying the recently published approach of adjusting the GaInSb layer thickness in the active region to reduce the valence intersubband absorption. The W-quantum well design adjustment and the optimization of the electron injector, to rebalance the electron and hole concentrations in the active quantum wells, improved the device performance, yielding room temperature current densities as low as 0.5 kA/cm$^2$ for broad area devices under pulsed operation. As a direct result of this improvement the long wavelength limit for GaSb-based ICLs in continuous wave operation could be extended. For an epi-side down mounted 23 $\mu$m wide and 2 mm long device with 9 active stages and high-reflectivity back facet the threshold power is below 1 W and the optical output power is over 25 mW at 20$^\circ$C in continuous-wave mode. Such low-threshold and low-power consumption interband cascade lasers are especially attractive for mobile and compact sensing systems

Influence of Multiple Donor Renal Arteries on the Outcome and Graft Survival in Deceased Donor Kidney Transplantation

Aim: Complex arterial reconstruction in kidney transplantation (KT) using kidneys from deceased donors (DD) warrants additional study since little is known about the effects on the mid- and long-term outcome and graft survival. Methods: A total of 451 patients receiving deceased donor KT in our department between 1993 and 2017 were included in our study. Patients were divided into three groups according to the number of arteries and anastomosis: (A) 1 renal artery, 1 arterial anastomosis (N = 369); (B) >1 renal artery, 1 arterial anastomosis (N = 47); and (C) >1 renal artery, >1 arterial anastomosis (N = 35). Furthermore, the influence of localization of the arterial anastomosis (common iliac artery (CIA), versus non-CIA) was analyzed. Clinicopathological characteristics, outcome, and graft and patient survival of all groups were compared retrospectively. Results: With growing vascular complexity, the time of warm ischemia increased significantly (groups A, B, and C: 40 ± 19 min, 45 ± 19 min, and 50 ± 17 min, respectively; p = 0.006). Furthermore, the duration of operation was prolonged, although this did not reach significance (groups A, B, and C: 175 ± 98 min, 180 ± 35 min, and 210 ± 43 min, respectively; p = 0.352). There were no significant differences regarding surgical complications, post-transplant kidney function (delayed graft function, initial non-function, episodes of acute rejection), or long-term graft survival. Regarding the localization of the arterial anastomosis, non-CIA was an independent prognostic factor for deep vein thrombosis in multivariate analysis (CIA versus non-CIA: OR 11.551; 95% CI, 1.218–109.554; p = 0.033). Conclusion: Multiple-donor renal arteries should not be considered a contraindication to deceased KT, as morbidity rates and long-term outcomes seem to be comparable with grafts with single arteries and less complex anastomoses

Activation of the Syk tyrosine kinase is insufficient for downstream signal transduction in B lymphocytes

BACKGROUND: Immature B lymphocytes and certain B cell lymphomas undergo apoptotic cell death following activation of the B cell antigen receptor (BCR) signal transduction pathway. Several biochemical changes occur in response to BCR engagement, including activation of the Syk tyrosine kinase. Although Syk activation appears to be necessary for some downstream biochemical and cellular responses, the signaling events that precede Syk activation remain ill defined. In addition, the requirements for complete activation of the Syk-dependent signaling step remain to be elucidated. RESULTS: A mutant form of Syk carrying a combination of a K395A substitution in the kinase domain and substitutions of three phenylalanines (3F) for the three C-terminal tyrosines was expressed in a murine B cell lymphoma cell line, BCL(1).3B3 to interfere with normal Syk regulation as a means to examine the Syk activation step in BCR signaling. Introduction of this kinase-inactive mutant led to the constitutive activation of the endogenous wildtype Syk enzyme in the absence of receptor engagement through a 'dominant-positive' effect. Under these conditions, Syk kinase activation occurred in the absence of phosphorylation on Syk tyrosine residues. Although Syk appears to be required for BCR-induced apoptosis in several systems, no increase in spontaneous cell death was observed in these cells. Surprisingly, although the endogenous Syk kinase was enzymatically active, no enhancement in the phosphorylation of cytoplasmic proteins, including phospholipase Cγ2 (PLCγ2), a direct Syk target, was observed. CONCLUSION: These data indicate that activation of Syk kinase enzymatic activity is insufficient for Syk-dependent signal transduction. This observation suggests that other events are required for efficient signaling. We speculate that localization of the active enzyme to a receptor complex specifically assembled for signal transduction may be the missing event

Rate of molecular transfer of allyl alcohol across an AOT surfactant layer using muon spin spectroscopy

The transfer rate of a probe molecule across the interfacial layer of a water-in-oil (w/o) microemulsion was investigated using a combination of Transverse Field muon spin rotation (TF- μSR), Avoided Level Crossing muon spin resonance (ALC-μSR) and Monte Carlo simulations. Reverse microemulsions consist of nanometer sized water droplets dispersed in an apolar solvent separated by a surfactant monolayer. Although the thermodynamic, static model of these systems has been well described, our understanding of their dynamics is currently incomplete. For example, what is the rate of solute transfer between the aqueous and apolar solvents, and how this is influenced by the structure of the interface? With an appropriate choice of system and probe molecule, µSR offers a unique opportunity to directly probe these interfacial transfer dynamics. Here, we have employed a well characterized w/o microemulsion stabilized by bis(2-ethylhexyl) sodium sulphosuccinate (Aerosol OT), with allyl alcohol (CH2=CH-CH2-OH, AA) as the probe. Resonances due to both muoniated radicals, CMuH2-C*H-CH2-OH and C*H2-CHMu-CH2-OH, were observed with the former being the dominant species. All resonances displayed solvent dependence, with those in the microemulsion observed as a single resonance located at intermediate magnetic fields to those present in either of the pure solvents. Observation of a single resonance is strong evidence for interfacial transfer being in the fast exchange limit. Monte-Carlo calculations of the ΔM=0 ALC resonances are consistent with the experimental data, indicating exchange rates greater than 109 s-1, placing the rate of interfacial transfer at the diffusion limit

Interband cascade laser arrays for simultaneous and selective analysis of C1-C5 hydrocarbons in the petrochemical industry

Funding: European Union Horizon 2020 research and innovation programme under grant agreement No 636930 (iCspec).The detection and measurement of hydrocarbons is of high interest for a variety of applications, for example within the oil & gas industry from extraction throughout the complete refining process, as well as for environmental monitoring and for portable safety devices. This paper presents a highly sensitive, selective and robust tunable laser analyzer that has the capability to analyze several components in a gas sample stream. More specifically, a multi-gas system for simultaneous detection of C1 to iC5 hydrocarbons, using a room temperature distributed feedback interband cascade laser array, emitting in the 3.3 micrometer band has been realized. It combines all the advantages of the tunable laser spectroscopy method for a fast, sensitive and selective in-line multicomponent tunable laser analyzer. Capable of continuous and milliseconds fast monitoring of C1-iC5 hydrocarbon compositions in a process stream, the analyzer requires no consumables (e.g. purging, carrier gas) and no in-field calibration, enabling a low cost of ownership for the analyzer. The system was built, based on an industrial GasEye series platform and deployed for the first time in field at Preem refinery in Lysekil, Sweden in autumn 2018. Results of the measurement campaign and comparison with gas chromatography instrumentation is presented.PostprintPeer reviewe

Double-waveguide interband cascade laser with dual wavelength emission

We are grateful for receiving financial support within the BMBF Project “LASELO” (FKZ: 13N13773).Interband cascade lasers (ICLs) with dual wavelength emission have been realized by utilizing two spatially separated active regions in the same device. The two wavelengths (3.1 and 3.7 µm) were chosen in order to demonstrate that the usual spectral gain bandwidth of an ICL can be overcome. At 20°C, threshold current densities as low as 215 A/cm² (short wavelength) and 158 A/cm² (long wavelength) could be achieved in pulsed mode. It was possible for an epi-up mounted device to maintain dual-wavelength continuous-wave emission up to 0°C. Despite the longer wavelength emission being suppressed at higher temperatures, the shorter wavelength maintained an output power of more than 10 mW at 15°C.PostprintPeer reviewe

Components of the antigen processing and presentation pathway revealed by gene expression microarray analysis following B cell antigen receptor (BCR) stimulation

BACKGROUND: Activation of naïve B lymphocytes by extracellular ligands, e.g. antigen, lipopolysaccharide (LPS) and CD40 ligand, induces a combination of common and ligand-specific phenotypic changes through complex signal transduction pathways. For example, although all three of these ligands induce proliferation, only stimulation through the B cell antigen receptor (BCR) induces apoptosis in resting splenic B cells. In order to define the common and unique biological responses to ligand stimulation, we compared the gene expression changes induced in normal primary B cells by a panel of ligands using cDNA microarrays and a statistical approach, CLASSIFI (Cluster Assignment for Biological Inference), which identifies significant co-clustering of genes with similar Gene Ontology™ annotation. RESULTS: CLASSIFI analysis revealed an overrepresentation of genes involved in ion and vesicle transport, including multiple components of the proton pump, in the BCR-specific gene cluster, suggesting that activation of antigen processing and presentation pathways is a major biological response to antigen receptor stimulation. Proton pump components that were not included in the initial microarray data set were also upregulated in response to BCR stimulation in follow up experiments. MHC Class II expression was found to be maintained specifically in response to BCR stimulation. Furthermore, ligand-specific internalization of the BCR, a first step in B cell antigen processing and presentation, was demonstrated. CONCLUSION: These observations provide experimental validation of the computational approach implemented in CLASSIFI, demonstrating that CLASSIFI-based gene expression cluster analysis is an effective data mining tool to identify biological processes that correlate with the experimental conditional variables. Furthermore, this analysis has identified at least thirty-eight candidate components of the B cell antigen processing and presentation pathway and sets the stage for future studies focused on a better understanding of the components involved in and unique to B cell antigen processing and presentation