Complement protein levels in plasma astrocyte-derived exosomes are abnormal in conversion from mild cognitive impairment to Alzheimer's disease dementia.

IntroductionLevels of complement proteins (CPs) in plasma astrocyte-derived exosomes (ADEs) that are abnormal in Alzheimer's disease (AD) have not been assessed in mild cognitive impairment (MCI).MethodsParticipants (n = 20 per group) had either MCI converting to dementia within 3 years (MCIC), MCI remaining stable over 3 years (MCIS), Alzheimer's disease, or were controls. CPs of ADEs isolated from plasmas by anti-human glutamine aspartate transporter antibody absorption were quantified by ELISAs.ResultsADE levels of C1q and C4b of the classical pathway, factor D and fragment Bb of the alternative pathway, and C5b, C3b, and C5b-C9 of both pathways were significantly higher in patients with MCIC than those with MCIS. ADE levels of inhibitory CPs decay-accelerating factor, CD46, CD59, and type 1 complement receptor were significantly lower in patients with MCIC than those with MCIS.DiscussionADE CPs are components of neurotoxic neuroinflammation that may be predictive biomarkers of MCI conversion to Alzheimer's disease

Data from a Static-Thrust Investigation of Large-Scale General Research VTOL-STOL Model in Ground Effect

The model was tested at two different elevations with the wing pivot at 1.008 and 2.425 propeller diameters above the ground. The slipstream of the propellers was deflected by tilting the wing and propellers, by deflections of large-chord trailing-edge flaps, and by combinations of flap deflection and wing tilt. Tests were conducted over a range of propeller disk loadings from 7.41 to 29.70 pounds per square foot. Force data for the complete model and pressure distributions for the wing and flaps behind one propeller were recorded and are presented in tabular form without analysis

Journal of Experimental & Clinical Assisted Reproduction: shaping the future of research and practice in reproductive endocrinology/infertility

Journal of Experimental & Clinical Assisted Reproduction is an open access, online, peer-review journal publishing papers on all aspects of research into reproductive endocrinology, infertility, bioethics and the advanced reproductive technologies. The journal reports on important developments impacting the field of human reproductive medicine and surgery. The field exists as a sub-specialty of obstetrics & gynecology, focusing on the diagnosis and treatment of complex human reproductive problems. The continued growth of this relatively new field depends on quality research by proven scientists as well as junior investigators who, together, make contributions to this area of medical and surgical practice. The publishing revolution made possible by internet technology presages a bright future for continued interdisciplinary collaboration among researchers. Against this background, Journal of Experimental & Clinical Assisted Reproduction exists for the scientific community to facilitate this scholarly dialogue

Paroxysmal Bundle Branch Block of Supraventricular Origin: A Possible Source of Misdiagnosis in Detecting Ventricular Tachycardia Using Time Domain Analyses of Intraventricular Electrograms

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/72385/1/j.1540-8159.1990.tb02060.x.pd

Awareness and bispectral index (BIS) monitoring in mechanically ventilated patients in the emergency department and intensive care unit: A systematic review protocol

INTRODUCTION: Accidental awareness with recall is one of the most feared complications for patients undergoing general anaesthesia and can lead to post-traumatic stress disorder in up to 70% of patients experiencing it. To reduce the incidence of awareness with recall, the bispectral index monitor is recommended for patients receiving total intravenous anaesthetics, especially those receiving neuromuscular blockers. While extensive investigation into awareness and bispectral index monitoring has occurred for operating room patients, this has not extended to other clinical arenas where sedated and mechanically ventilated patients are cared for, namely the intensive care unit and emergency department. The purpose of this systematic review is to assess the world\u27s literature to determine the incidence of awareness with paralysis in mechanically ventilated patients and the impact of bispectral index monitoring for reducing this complication. METHODS AND ANALYSIS: Randomised trials and non-randomised studies are eligible for inclusion. With aid from a medical librarian, an electronic search will include Ovid Medline, Embase.com, Scopus, Cochrane Database of Systematic Reviews and Cochrane Central Register of Controlled Trials. To find data published in abstract form, literature from professional society conferences (2010-2019) will be manually searched. Two authors will independently review search results and consensus will be reached with assistance from a third author, as needed. Heterogeneity and publication bias will be assessed and reported. If possible and appropriate, a meta-analysis of the data will be conducted for quantitative data analysis. ETHICS AND DISSEMINATION: The proposed systematic review does not require ethical approval, as it is conducted at the study level and does not involve individual patient-level data. Results will be disseminated by data sharing via academically established means, presentation at local and national scientific meetings and publication as a peer-reviewed manuscript. PROSPERO REGISTRATION NUMBER: The protocol has been submitted to International Prospective Register of Systematic Reviews and is awaiting registration

Assessing Neuronal and Astrocyte Derived Exosomes From Individuals With Mild Traumatic Brain Injury for Markers of Neurodegeneration and Cytotoxic Activity.

Mild traumatic brain injury (mTBI) disproportionately affects military service members and is very difficult to diagnose. To-date, there is currently no blood-based, diagnostic biomarker for mTBI cases with persistent post concussive symptoms. To examine the potential of neuronally-derived (NDE) and astrocytic-derived (ADE) exosome cargo proteins as biomarkers of chronic mTBI in younger adults, we examined plasma exosomes from a prospective longitudinal study of combat-related risk and resilience, marine resiliency study II (MRSII). After return from a combat-deployment participants were interviewed to assess TBI exposure while on deployment. Plasma exosomes from military service members with mTBI (mean age, 21.7 years, n = 19, avg. days since injury 151), and age-matched, controls (deployed service members who did not endorse a deployment-related TBI or a pre-deployment history of TBI; mean age, 21.95 years, n = 20) were precipitated and enriched against a neuronal adhesion protein, L1-CAM, and an astrocyte marker, glutamine aspartate transporter (GLAST) using magnetic beads to immunocapture the proteins and subsequently selected by fluorescent activated cell sorting (FACS). Extracted protein cargo from NDE and ADE preparations were quantified for protein levels implicated in TBI neuropathology by standard ELISAs and on the ultra-sensitive single molecule assay (Simoa) platform. Plasma NDE and ADE levels of Aβ42 were significantly higher while plasma NDE and ADE levels of the postsynaptic protein, neurogranin (NRGN) were significantly lower in participants endorsing mTBI exposure compared to controls with no TBI history. Plasma NDE and ADE levels of Aβ40, total tau, and neurofilament light (NFL), P-T181-tau, P-S396-tau were either undetectable or not significantly different between the two groups. In an effort to understand the pathogenetic potential of NDE and ADE cargo proteins, neuron-like cultures were treated with NDE and ADE preparations from TBI and non-TBI groups. Lastly, we determined that plasma NDE but not ADE cargo proteins from mTBI samples were found to be toxic to neuron-like recipient cells in vitro. These data support the presence of markers of neurodegeneration in NDEs of mTBI and suggest that these NDEs can be used as tools to identify pathogenic mechanisms of TBI

Statistical validation of new ventricular tachycardia detection schemes

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/28828/1/0000662.pd

Processing and Technology of Fruits and Vegetables, 1960

Tomato Variety Evaluation for Processing, 1961 / W. A. Gould, J. R. Geisman and Wade Schulte -- Evaluation of Sweet Corn Varieties for Processing / J. R. Geisman and W. A. Gould -- Small Fruit Variety Evaluation Studies for Freezing / D. R. Davis and H. L. Stammer -- Evaluation of Apples for Processing. I. Fruit Juice Blends / D. R. Davis and H. L. Stammer -- Evaluation of Apples for Processing. II. Canned Apple Slices / D. R. Davis and H. L. Stammer -- Evaluation of Apples for Processing. III. Frozen Apple Slices / D. R. Davis and H. L. Stammer -- Evaluation of Apples for Processing. IV. Frozen Fruit Pies / D. R. Davis and H. L. Stammer -- A Study of Several Varieties of Pumpkin and Squash for Canning and Freezing for Use in Pies / Robert H. Clayton, J. R. Geisman and W. A. Gould -- Factors Effecting the Consistency of Cream Style Corn / D. R. Davis and W. A. Gould -- The Objective Measurement of Tomato Juice Consistency / Robert Kluter and W. A. Gould -- A Method for the Detection of Drosophila Fly Eggs and Larvae in Tomato Products / J. R. Geisman and Winston D. Bash -- A Chemical Study of Flavor and Flavor Substances in Tomatoes / John Hal Johnson and W. A. Gould -- Tannin Content Effects Grape Juice Quality / D. R. Davis and H. L. Stammer -- pH Survey for Tomatoes in Ohio / W. D. Bash -- Flavor Studies with Sauerkraut / J. R. Geisman, S. S. Verma and W. A. Gould -- The Effect of Fill Weight on Drained Weight of Canned Tomatoes / Wade A. Schulte and W. A. Gould -- A New Method for the Manufacture of Apple Sirup / M. P. Baldauf, D. R. Davis and H. L. Stammer -- Studies on Color Retention in Canned R.T.P. Cherries / D. R. Davis and H. L. Stammer -- Clumping Studies in Canned Blueberries / D. R. Davis and H. L. Stammer -- Infra-red Peeling Studies. I. Apples. / L. Lafferty and W. A. Gould -- Infra-red Peeling Studies. II. Tomatoes. / W. A. Gould, Richard Leiss and Donall Streets -- The Effect of Water Holding Times and Temperatures on Quality of Tomatoes / Richard Leiss, Ernest Anderson and W. A. Gould -- Quality Attributes of Sweet Potatoes - Glass Packed / Donald A. Giesser and W. A. Gould -- A Study of Some of the Factors Effecting the Efficiency of Washing of Fruits and Vegetables. I. Tomatoes / W. A. Gould and J. R. Geisman -- A Study of Some of the Factors Effecting the Efficiency of Washing of Fruits and Vegetables. II. Sweet Corn / J. R. Geisman and W. A. Gould -- A Study of Alpha-keto Acids, Amino Acids, and Citric Acid in Eight Tomato Varieties, and Their Changes During Processing / Mokhtar M. Hamdy and W. A. Goul

Universal Reference RNA as a standard for microarray experiments

BACKGROUND: Obtaining reliable and reproducible two-color microarray gene expression data is critically important for understanding the biological significance of perturbations made on a cellular system. Microarray design, RNA preparation and labeling, hybridization conditions and data acquisition and analysis are variables difficult to simultaneously control. A useful tool for monitoring and controlling intra- and inter-experimental variation is Universal Reference RNA (URR), developed with the goal of providing hybridization signal at each microarray probe location (spot). Measuring signal at each spot as the ratio of experimental RNA to reference RNA targets, rather than relying on absolute signal intensity, decreases variability by normalizing signal output in any two-color hybridization experiment. RESULTS: Human, mouse and rat URR (UHRR, UMRR and URRR, respectively) were prepared from pools of RNA derived from individual cell lines representing different tissues. A variety of microarrays were used to determine percentage of spots hybridizing with URR and producing signal above a user defined threshold (microarray coverage). Microarray coverage was consistently greater than 80% for all arrays tested. We confirmed that individual cell lines contribute their own unique set of genes to URR, arguing for a pool of RNA from several cell lines as a better configuration for URR as opposed to a single cell line source for URR. Microarray coverage comparing two separately prepared batches each of UHRR, UMRR and URRR were highly correlated (Pearson's correlation coefficients of 0.97). CONCLUSION: Results of this study demonstrate that large quantities of pooled RNA from individual cell lines are reproducibly prepared and possess diverse gene representation. This type of reference provides a standard for reducing variation in microarray experiments and allows more reliable comparison of gene expression data within and between experiments and laboratories