203 research outputs found

    Isolated Spinach Ribulose-1,5-Bisphosphate Carboxylase/Oxgenase Large Subunit ε N-Methyltransferase and Method of Inactivating Ribulose-1,5-Bishosphatase ε N-Methyltransferase Activity

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    The gene sequence for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (LS)εN-methyltansferase (protein methylase III or Rubisco LSMT) from a plant which has a des(methyl) lysyl residue in the LS is disclosed. In addition, the full-length cDNA clones for Rubisco LSMT are disclosed. Transgenic plants and methods of producing same which have the Rubisco LSMT gene inserted into the DNA are also provided. Further, methods of inactivating the enzymatic activity of Rubisco LSMT are also disclosed

    Isolated Spinach Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Large Subunit \u3csup\u3eε\u3c/sup\u3eN-Methyltransferase and Method of Inactivating Ribulose-1,5-Bisphosphatase Carboxylase/Oxygenase Large Subunit \u3csup\u3eε\u3c/sup\u3eN-Methyltransferase Activity

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    The gene sequence for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (LS) .sup.ε N-methyltransferase (protein methylase III or Rubisco LSMT) from a plant which has a des(methyl) lysyl residue in the LS is disclosed. In addition, the full-length cDNA clones for Rubisco LSMT are disclosed. Transgenic plants and methods of producing same which have the Rubisco LSMT gene inserted into the DNA are also provided. Further, methods of inactivating the enzymatic activity of Rubisco LSMT are also disclosed

    Reflections on Undergraduate Research Mentoring

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    Cloning and Developmental Expression of Pea Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Large Subunit N-Methyltransferase

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    The gene sequence for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (LS) εN-methyltransferase (protein methylase III or Rubisco LSMT) is disclosed. This enzyme catalyzes methylation of the ε-amine of lysine-14 in the large subunit of Rubisco. In addition, a full-length cDNA clone for Rubisco LSMT is disclosed. Transgenic plants and methods of producing same which (1) have the Rubisco LSMT gene inserted into the DNA, and (2) have the Rubisco LSMT gene product or the action of the gene product deleted from the DNA are also provided. Further, methods of using the gene to selectively deliver desired agents to a plant are also disclosed

    Modified Rubisco Large Subunit N-Methyltransferase Useful for Targeting Molecules to the Active-Site Vicinity of Ribulose-1,5-Bisphosphate

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    The present invention generally relates to a modified Rubisco large subunit εN-Methyltransferase (Rubisco LSMT, or RLSMT). The present invention also relates to a modified RLSMT-carbonic anhydrase (RLSMT-CA). This modified RLSMT-CA improves the efficiency of the reduction of CO2 during photosynthesis, which may increase plant growth rates. The present invention also relates to nucleic acids encoding the modified RLSMT-CA or modified RLSMT. Also, the present invention relates to cells including the modified RLSMT-CA or modified RLSMT, plants containing the modified RLSMT-CA or modified RLSMT, and methods using compositions of the present invention. In addition, the present invention relates to antibodies conjugated to CA which may bind to Rubisco, and antibodies which bind a modified RLSMT-CA. The invention also relates to modified forms of the LS and SS of Rubisco where the modified forms are fusions with CA or biologically active fragments thereof. The present invention provides methods of altering Rubisco carboxylase activity and altering plant growth

    Inhibitors of Plant Peptide Deformylase for Use as Broad-Spectrum Herbicides and Methods for Identifying the Same

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    The invention relates to a method of identifying herbicides and to the use of inhibitors of plant peptide deformylase as broad spectrum herbicides

    Inhibitors of Plant Peptide Deformylase for Use as Broad-Spectrum Herbicides and Methods for Identifying the Same

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    The invention relates to a method of identifying herbicides and to the use of inhibitors of plant peptide deformylase as broad spectrum herbicides

    Inhibitor of Plant Peptide Deformylase for Use as Broad-Spectrum Herbicides and Method for Identifying the Same

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    The invention relates to a method of identifying herbicides and to the use of inhibitors of plant peptide deformylase as broad spectrum herbicides

    Crystallization and Structure of a Plant Peptide Deformylase

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    This invention relates to the crystal structure of a plant peptide deformylase polypeptide and methods of using the structure to design compounds that modulate the activity of the polypeptide

    Crystallization and Structure of a Plant Peptide Deformylase

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    This invention relates to the crystal structure of a plant peptide deformylase polypeptide and methods of using the structure to design compounds that modulate the activity of the polypeptide
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