Neurotrophin and Trk expression by cells of the human lamina cribrosa following oxygen-glucose deprivation-6

<p><b>Copyright information:</b></p><p>Taken from "Neurotrophin and Trk expression by cells of the human lamina cribrosa following oxygen-glucose deprivation"</p><p>BMC Neuroscience 2004;5():51-51.</p><p>Published online 3 Dec 2004</p><p>PMCID:PMC539236.</p><p>Copyright © 2004 Lambert et al; licensee BioMed Central Ltd.</p> by recovery for 24 hours (OGD+R). NGF and BDNF secretion was determined by immunoassay of concentrated conditioned media from three LC cell lines and three ONH astrocyte cell lines. A NT standard was included in each assay and was used to generate a standard curve. Data are reported as percent of the control ± SEM (n = 3). * indicates p < 0.016 for OGD compared to C and OGD+R; † indicates p < 0.016 for OGD+R compared to C (one way analysis of variance [ANOVA] followed by validation using Student-Newman-Keuls tests)

Neurotrophin and Trk expression by cells of the human lamina cribrosa following oxygen-glucose deprivation-1

<p><b>Copyright information:</b></p><p>Taken from "Neurotrophin and Trk expression by cells of the human lamina cribrosa following oxygen-glucose deprivation"</p><p>BMC Neuroscience 2004;5():51-51.</p><p>Published online 3 Dec 2004</p><p>PMCID:PMC539236.</p><p>Copyright © 2004 Lambert et al; licensee BioMed Central Ltd.</p> hours (OGD+R). Cells exposed to growth media and 95% air/5% COfor 48 hours served as controls (C). Cell lysate was separated by SDS-PAGE and the density of unsaturated bands was measured for each blot. A representative blot for each NT is shown. Band density reported as mean percent of the control ± SEM (n = 3) is shown graphically beneath the blots. * indicates p < 0.016 for OGD compared to C and OGD+R; † indicates p < 0.016 for OGD+R compared to C (one way analysis of variance [ANOVA] followed by validation using Student-Newman-Keuls tests)

Neurotrophin and Trk expression by cells of the human lamina cribrosa following oxygen-glucose deprivation-5

<p><b>Copyright information:</b></p><p>Taken from "Neurotrophin and Trk expression by cells of the human lamina cribrosa following oxygen-glucose deprivation"</p><p>BMC Neuroscience 2004;5():51-51.</p><p>Published online 3 Dec 2004</p><p>PMCID:PMC539236.</p><p>Copyright © 2004 Lambert et al; licensee BioMed Central Ltd.</p> by recovery for 24 hours (OGD+R). Cells exposed to growth media and 95% air/5% COfor 48 hours served as controls (C). Cell lysate was separated by SDS-PAGE and the density of unsaturated bands was measured for each blot. A representative blot for each phospho-Trk receptor isoform is shown. Band density reported as mean percent of the control ± SEM (n = 3) is shown graphically beneath the blots. * indicates p < 0.016 for OGD compared to C and OGD+R; † indicates p < 0.016 for OGD+R compared to C (one way analysis of variance [ANOVA] followed by validation using Student-Newman-Keuls tests)

Neurotrophin and Trk expression by cells of the human lamina cribrosa following oxygen-glucose deprivation-0

<p><b>Copyright information:</b></p><p>Taken from "Neurotrophin and Trk expression by cells of the human lamina cribrosa following oxygen-glucose deprivation"</p><p>BMC Neuroscience 2004;5():51-51.</p><p>Published online 3 Dec 2004</p><p>PMCID:PMC539236.</p><p>Copyright © 2004 Lambert et al; licensee BioMed Central Ltd.</p>ng OGD or control conditions for the time points indicated. Three LC cell lines and three ONH astrocyte cell lines were assayed per experiment. A standard curve was generated for each cell line in order to calculate cell metabolism/cell number. Data shown as percent of the control and represents the mean ± SEM (n = 3). * indicates p < 0.008 (one way analysis of variance [ANOVA] followed by validation using Student-Newman-Keuls tests)