The effect of a density difference on shear-layer instability

Measurements of mass flow rate and mean density have been made in separated laminar boundary layers with large transverse density gradients. Two-dimensional shear layers were formed by exhausting a half-jet of one gas into a reservoir of another gas with a different molecular weight. Two freons with a density ratio of 1-98 and unusual properties which permitted the measurement of the mass flow rate with a single hot wire were used. A n analysis of the mass flow rate fluctuations showed that a negative density gradient (i.e. light gas flowing into heavy) increases the amplification rate of the instability oscillations and reduces the frequency and wave number. Opposite trends were observed when the density gradient was positive. These findings are in agreement with recent theoretical predictions

Influence of school community and fitness on prevalence of overweight in Australian school children

AbstractThe study objectives were (1) to determine the variation in prevalence of overweight between school communities, (2) to evaluate the relationship between cardiorespiratory fitness and the probability of being overweight among different school communities, and (3) to test whether this relationship varies between school communities. Using a repeated cross-sectional design, data from 31,424 (15,298 girls, 16,126 boys) Australian school children who had objective assessments of body composition and physical performance were used. Ninety-one schools located across 5 states and territories were included. Independent samples were taken across 12 school years (2000–2011). Analysis used generalised linear mixed models in R with a two-level hierarchical structure—children, nested within school communities. Predictor variables considered were: level 1—gender, age, cardiorespiratory fitness and year of measurement; level 2—school community. A total of 24.6% of the children were overweight and 69% were of low fitness. Variation in the prevalence of overweight between school communities was significant, ranging from 19% to 34%. The probability of being overweight was negatively associated with increasing cardiorespiratory fitness. The relationship was steepest at low fitness and varied markedly between school communities. Children of low fitness had probabilities of being overweight ranging between 26% and 75% depending on school community, whereas those of high fitness had probabilities of <2%. Our findings suggest that most might be gained from a public health perspective by focusing intervention on the least fit children in the worst-performing communities

Synthesis and Antiviral Activity of Fatty Acyl Conjugates of Remdesivir Against Severe Acute Respiratory Syndrome Coronavirus 2 and Ebola Virus

We report here the synthesis, purification, and characterization of mono- and di-fatty acyl conjugates of remdesivir (RDV) and their in vitro antiviral activity against SAR-CoV-2, an Ebola virus transcription- and replication-competent virus-like particle (trVLP) system, and infectious Ebola virus. The most potent monofatty acyl conjugate was 4b, containing a 4-oxatetradecanolyl at the 3′ position. Monofatty acyl conjugates, 3′-O-tetradecanoyl (4a) (IC50(VeroE6) = 2.3 μM; IC50(Calu3) = 0.24 μM), 3′-O-4-oxatetradodecanoyl (4b) (IC50(VeroE6) = 2.0 μM; IC50(Calu3) = 0.18 μM), and 3′-O-(12-ethylthiododecanoyl) (4e) (IC50(VeroE6) = 2.4 μM; IC50(Calu3) = 0.25 μM) derivatives exhibited less activity than RDV (IC50(VeroE6) = 0.85 μM; IC50(Calu3) = 0.06 μM) in both VeroE6 and Calu3 cells. Difatty acylation led to a significant reduction in the antiviral activity of RDV (as shown in conjugates 5a and 5b) against SARS-CoV-2 when compared with monofatty acylation (3a-e and 4a-e). About 77.9% of 4c remained intact after 4 h incubation with human plasma while only 47% of parent RDV was observed at the 2 h time point. The results clearly indicate the effectiveness of fatty acylation to improve the half-life of RDV. The antiviral activities of a number of monofatty acyl conjugates of RDV, such as 3b, 3e, and 4b, were comparable with RDV against the Ebola trVLP system. Meanwhile, the corresponding physical mixtures of RDV and fatty acids 6a and 6b showed 1.6 to 2.2 times less antiviral activity than the corresponding conjugates, 4a and 4c, respectively, against SARS-CoV-2 in VeroE6 cells. A significant reduction in viral RNA synthesis was observed for selected compounds 3a and 4b consistent with the IC50 results. These studies indicate the potential of these compounds as long-acting antiviral agents or prodrugs of RDV

Population genomics of domestic and wild yeasts

The natural genetics of an organism is determined by the distribution of sequences of its genome. Here we present one- to four-fold, with some deeper, coverage of the genome sequences of over seventy isolates of the domesticated baker&#x27;s yeast, _Saccharomyces cerevisiae_, and its closest relative, the wild _S. paradoxus_, which has never been associated with human activity. These were collected from numerous geographic locations and sources (including wild, clinical, baking, wine, laboratory and food spoilage). These sequences provide an unprecedented view of the population structure, natural (and artificial) selection and genome evolution in these species. Variation in gene content, SNPs, indels, copy numbers and transposable elements provide insights into the evolution of different lineages. Phenotypic variation broadly correlates with global genome-wide phylogenetic relationships however there is no correlation with source. _S. paradoxus_ populations are well delineated along geographic boundaries while the variation among worldwide _S. cerevisiae_ isolates show less differentiation and is comparable to a single _S. paradoxus_ population. Rather than one or two domestication events leading to the extant baker&#x27;s yeasts, the population structure of _S. cerevisiae_ shows a few well defined geographically isolated lineages and many different mosaics of these lineages, supporting the notion that human influence provided the opportunity for outbreeding and production of new combinations of pre-existing variation

Surface cloud forcing in the East Pacific stratus deck/cold tongue/ITCZ complex

Author Posting. © American Meteorological Society 2006. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Climate 19 (2006): 392–409, doi:10.1175/JCLI3620.1.Data from the Eastern Pacific Investigation of Climate Studies (EPIC) mooring array are used to evaluate the annual cycle of surface cloud forcing in the far eastern Pacific stratus cloud deck/cold tongue/intertropical convergence zone complex. Data include downwelling surface solar and longwave radiation from 10 EPIC-enhanced Tropical Atmosphere Ocean (TAO) moorings from 8°S, 95°W to 12°N, 95°W, and the Woods Hole Improved Meteorology (IMET) mooring in the stratus cloud deck region at 20°S, 85°W. Surface cloud forcing is defined as the observed downwelling radiation at the surface minus the clear-sky value. Solar cloud forcing and longwave cloud forcing are anticorrelated at all latitudes from 12°N to 20°S: clouds tended to reduce the downward solar radiation and to a lesser extent increase the downward longwave radiation at the surface. The relative amount of solar radiation reduction and longwave increase depends upon cloud type and varies with latitude. A statistical relationship between solar and longwave surface cloud forcing is developed for rainy and dry periods and for the full record length in six latitudinal regions: northeast tropical warm pool, ITCZ, frontal zone, cold tongue, southern, and stratus deck regions. The buoy cloud forcing observations and empirical relations are compared with the International Satellite Cloud Climatology Project (ISCCP) radiative flux data (FD) dataset and are used as benchmarks to evaluate surface cloud forcing in the NCEP Reanalysis 2 (NCEP2) and 40-yr ECMWF Re-Analysis (ERA-40). ERA-40 and NCEP2 cloud forcing (both solar and longwave) showed large discrepancies with observations, being too large in the ITCZ and equatorial regions and too weak under the stratus deck at 20°S and north to the equator during the cool season from July to December. In particular the NCEP2 cloud forcing at the equator was nearly identical to the ITCZ region and thus had significantly larger solar cloud forcing and smaller longwave cloud forcing than observed. The net result of the solar and longwave cloud forcing deviations is that there is too little radiative warming in the ITCZ and southward to 8°S during the warm season and too much radiative warming under the stratus deck at 20°S and northward to the equator during the cold season.This research was supported by grants from the NOAA Office of Global Programs, Pan American Climate Studies

Equating accelerometer estimates among youth : the Rosetta Stone 2

Different accelerometer cutpoints used by different researchers often yields vastly different estimates of moderate-to-vigorous intensity physical activity (MVPA). This is recognized as cutpoint non-equivalence (CNE), which reduces the ability to accurately compare youth MVPA across studies. The objective of this research is to develop a cutpoint conversion system that standardizes minutes of MVPA for six different sets of published cutpoint

Parent-of-origin-specific allelic associations among 106 genomic loci for age at menarche.

Age at menarche is a marker of timing of puberty in females. It varies widely between individuals, is a heritable trait and is associated with risks for obesity, type 2 diabetes, cardiovascular disease, breast cancer and all-cause mortality. Studies of rare human disorders of puberty and animal models point to a complex hypothalamic-pituitary-hormonal regulation, but the mechanisms that determine pubertal timing and underlie its links to disease risk remain unclear. Here, using genome-wide and custom-genotyping arrays in up to 182,416 women of European descent from 57 studies, we found robust evidence (P < 5 × 10(-8)) for 123 signals at 106 genomic loci associated with age at menarche. Many loci were associated with other pubertal traits in both sexes, and there was substantial overlap with genes implicated in body mass index and various diseases, including rare disorders of puberty. Menarche signals were enriched in imprinted regions, with three loci (DLK1-WDR25, MKRN3-MAGEL2 and KCNK9) demonstrating parent-of-origin-specific associations concordant with known parental expression patterns. Pathway analyses implicated nuclear hormone receptors, particularly retinoic acid and γ-aminobutyric acid-B2 receptor signalling, among novel mechanisms that regulate pubertal timing in humans. Our findings suggest a genetic architecture involving at least hundreds of common variants in the coordinated timing of the pubertal transition