Distribution of polymorphic, monomorphic and failed SNPs mapped to the dog (<b><i>Canis lupus familiaris</i></b><b>) genome (shown as three rows, </b><b><i>n</i></b><b> = 193, </b><b><i>n</i></b><b> = 33,131 and </b><b><i>n</i></b><b> = 136,903 respectively).</b>

<p>Distribution of polymorphic, monomorphic and failed SNPs mapped to the dog (<b><i>Canis lupus familiaris</i></b><b>) genome (shown as three rows, </b><b><i>n</i></b><b> = 193, </b><b><i>n</i></b><b> = 33,131 and </b><b><i>n</i></b><b> = 136,903 respectively).</b></p

Examples of single nucleotide polymorphisms (SNPs) from the canine SNP chip that cross-amplify in Antarctic fur seals.

<p>Each point represents a single sample. ‘Norm R’ (y-axis) is the normalized sum of the intensities of the two channels (Cy3 and Cy5). ‘Norm Theta’ (x-axis) is ((2 / p)Tan)1 (Cy5 / Cy3)) where a value near 0 represents a homozygote for allele A (denoted by red points) and a value near 1 represents a homozygote for allele B (denoted by blue points). Heterozygotes fall approximately mid-way between these values and are denoted by purple points. The numbers of samples called by GenomeStudio for each of the three possible genotypes are shown below the x-axis. (a–d) Classical three-cluster patterns for SNPs considered successful and polymorphic; (e) A monomorphic SNP; (f) A locus that failed to yield an interpretable assay and was thus classified as a genotyping failure.</p

Example genotypes obtained for locus BICF2G630131208 using KASP chemistry and an ABI Step-One real-time PCR machine.

<p>The three discrete clusters of heterozygous and alternative homozygous genotypes denoted by green, red and blue points respectively include all 24 Antarctic fur seals as well as positive canine control samples. The two black squares indicate negative controls.</p

Distribution of the distance between each SNPs and its nearest gene in the dog, shown for polymorphic, monomorphic and failed SNPs.

<p>Distribution of the distance between each SNPs and its nearest gene in the dog, shown for polymorphic, monomorphic and failed SNPs.</p

Details of seven polymorphic SNPs revealing homology to the <i>Arctocephalus gazella</i> transcriptome.

<p>Details of seven polymorphic SNPs revealing homology to the <i>Arctocephalus gazella</i> transcriptome.</p