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Do diatomaceous earths have potential as grain protectants for small-holder farmers in sub-Saharan Africa? The case of Tanzania
Participatory on-farm field trials were set up over three storage seasons, from 2002 to 2005, in different agroecological zones of Tanzania to compare the efficacy of the enhanced diatomaceous earths (DEs) Protect-Its and Dryacides alone or combined with the pyrethroid permethrin. Other treatments included three commercially available synthetic chemical dilute dusts, containing 1.6% pirimiphos-methyl and 0.3% permethrin (Actellic Super and Stocal Super from different manufacturers) and 1% fenitrothion and 0.13% deltamethrin (Shumba Super); traditional protectants; and a locally available DE collected from Kagera in north-west Tanzania. Treatments were applied to maize and sorghum grain and dried beans. Insect pests are the main threat during storage, which in Tanzania includes the devastating larger grain borer, Prostephanus truncatus. All the grain protectants, except the traditional ones, kept damage incidence well below that of the untreated controls, and usually below 10% for periods of 40 weeks of storage. Exceptions occurred when grain was badly infested prior to treatment, in which case Actellic Super dust was more effective than the DEs. Very little difference in damage was observed between the DE treatments until 40 weeks of storage. In addition to the commercially available synthetic grain protectants, Protect-Its 0.25% w/w or Protect-Its 0.1% w/w plus permethrin at 2 mg/kg can be recommended to protect dry un-infested, winnowed maize and sorghum grain that is to be stored on-farm in sacks or woven granary baskets for periods of 4 months or more in Tanzania. Beans can be protected with lower application rates of Protect-Its 0.05% w/w or Dryacides 0.1% w/w. The study also demonstrated that Actellic Super dust obtained from an approved source and applied according to the manufacturer’s recommendations is effective in protecting stored maize, sorghum and beans for periods of at least 40 weeks—contrary to many of the suggestions that this product is no longer effective in Tanzania
Utilization of dried blood spot specimens can expedite nationwide surveillance of HIV drug resistance in resource-limited settings.
INTRODUCTION:Surveillance of HIV drug resistance (HIVDR) is crucial to ensuring the continued success of antiretroviral therapy (ART) programs. With the concern of reduced genotyping sensitivity of HIV on dried blood spots (DBS), DBS for HIVDR surveillance have been limited to ART-naïve populations. To investigate if DBS under certain conditions may also be a feasible sample type for HIVDR testing in ART patients, we piloted nationwide surveys for HIVDR among ART patients using DBS in two African countries with rapid scale-up of ART. METHODS:EDTA-venous blood was collected to prepare DBS from adult and pediatric ART patients receiving treatment during the previous 12-36 months. DBS were stored at ambient temperature for two weeks and then at -80°C until shipment at ambient temperature to the WHO-designated Specialized HIVDR Laboratory at CDC in Atlanta. Viral load (VL) was determined using NucliSENS EasyQ® HIV-1 v2.0 kits; HIVDR genotyping was performed using the ATCC HIV-1 Drug Resistance Genotyping kits. RESULTS:DBS were collected from 1,368 and 1,202 ART patients; 244 and 255 these specimens had VL ≥1,000 copies/mL in Kenya and Tanzania, respectively. The overall genotyping rate of those DBS with VL ≥1,000 copies/mL was 93.0% (95% CI: 89.1%-95.6%) in Kenya and 91.8% (87.7%-94.6%) in Tanzania. The turnaround times for the HIVDR surveys from the time of collecting DBS to completing laboratory testing were 6.5 months and 9.3 months for the Kenya and Tanzania surveys, respectively. CONCLUSIONS:The study demonstrates a favorable outcome of using DBS for nationwide surveillance of HIVDR in ART patients. Our results confirm that DBS collected and stored at ambient temperature for two weeks, and shipped with routine courier services are a reliable sample type for large-scale surveillance of acquired HIVDR