Productividad y calidad de cuatro híbridos de espárrago verde (Asparagus officinalis L. var. altilis) en invernadero

El espárrago constituye una alternativa productiva que requiere de la optimización del manejo en los distintos eslabones de la cadena agroalimentaria, como así también de estrategias para la extensión del calendario de oferta. El objetivo de este trabajo fue evaluar algunas estrategias tendientes a la optimización de la productividad de la cadena espárrago. Se evaluó la productividad neta (PFN), número de turiones comerciales producidos (NT) y distribución de calibres (jumbo, extra large, large, medium, small y asparagina), en invernadero de los genotipos masculinos italianos: H668, Marte y Giove y el testigo americano UC157. Comparar su comportamiento en poscosecha, evaluando la evolución del peso fresco, mediante el empleo de diferentes alternativas de valorización: largos de los turiones (22 y 12 cm) y presentaciones (ACF: atados con film y ASF: atados sin film), considerando un peso inicial de 500 g. Se efectuó un análisis multifactor Anova LSD test. En PFN fue obtenido: ‘Giove’ 5.096,5 kg ha-1, ‘UC157’ 5.062,5 kg ha-1, ‘H668’ 3.187,5 y ‘Marte’ 2.043,5 kg ha-1. En NT se destacaron: ‘UC157’ 322.300 kg ha-1, ‘Giove’ 267.500 kg ha-1, ‘H668’ 240.000 kg ha-1 y ‘Marte’ 166.250 kg ha-1. En poscosecha, el uso de film permitió conservar mejor los turiones procesados, demostrado por la menor disminución del peso fresco: ACF 201,73 g y ASF 299,21 g. ‘Giove’ y ‘UC157’ presentaron mejor comportamiento, conservando 264,82 y 267,25 g, respectivamente. Los turiones cortos, acondicionados en atados, perdieron mayor peso fresco que los largos durante el periodo de poscosecha (22 cm: 228,13 g y 12 cm: 272,81 g, de pérdida de peso, en relación a los 500 g iniciales, respectivamente). En síntesis, la productividad lograda, el anticipo y un adecuado acondicionamiento resultan alentadores para esta hortaliza

Unlipidated Outer Membrane Protein Omp16 (U-Omp16) from Brucella spp. as Nasal Adjuvant Induces a Th1 Immune Response and Modulates the Th2 Allergic Response to Cow's Milk Proteins

The discovery of novel mucosal adjuvants will help to develop new formulations to control infectious and allergic diseases. In this work we demonstrate that U-Omp16 from Brucella spp. delivered by the nasal route (i.n.) induced an inflammatory immune response in bronchoalveolar lavage (BAL) and lung tissues. Nasal co-administration of U-Omp16 with the model antigen (Ag) ovalbumin (OVA) increased the amount of Ag in lung tissues and induced OVA-specific systemic IgG and T helper (Th) 1 immune responses. The usefulness of U-Omp16 was also assessed in a mouse model of food allergy. U-Omp16 i.n. administration during sensitization ameliorated the hypersensitivity responses of sensitized mice upon oral exposure to Cow's Milk Protein (CMP), decreased clinical signs, reduced anti-CMP IgE serum antibodies and modulated the Th2 response in favor of Th1 immunity. Thus, U-Omp16 could be used as a broad Th1 mucosal adjuvant for different Ag formulations.Laboratorio de Investigaciones del Sistema Inmun

Unlipidated Outer Membrane Protein Omp16 (U-Omp16) from Brucella spp. as Nasal Adjuvant Induces a Th1 Immune Response and Modulates the Th2 Allergic Response to Cow's Milk Proteins

The discovery of novel mucosal adjuvants will help to develop new formulations to control infectious and allergic diseases. In this work we demonstrate that U-Omp16 from Brucella spp. delivered by the nasal route (i.n.) induced an inflammatory immune response in bronchoalveolar lavage (BAL) and lung tissues. Nasal co-administration of U-Omp16 with the model antigen (Ag) ovalbumin (OVA) increased the amount of Ag in lung tissues and induced OVA-specific systemic IgG and T helper (Th) 1 immune responses. The usefulness of U-Omp16 was also assessed in a mouse model of food allergy. U-Omp16 i.n. administration during sensitization ameliorated the hypersensitivity responses of sensitized mice upon oral exposure to Cow's Milk Protein (CMP), decreased clinical signs, reduced anti-CMP IgE serum antibodies and modulated the Th2 response in favor of Th1 immunity. Thus, U-Omp16 could be used as a broad Th1 mucosal adjuvant for different Ag formulations.Laboratorio de Investigaciones del Sistema Inmun

VIII Encuentro de Docentes e Investigadores en Historia del Diseño, la Arquitectura y la Ciudad

Acta de congresoLa conmemoración de los cien años de la Reforma Universitaria de 1918 se presentó como una ocasión propicia para debatir el rol de la historia, la teoría y la crítica en la formación y en la práctica profesional de diseñadores, arquitectos y urbanistas. En ese marco el VIII Encuentro de Docentes e Investigadores en Historia del Diseño, la Arquitectura y la Ciudad constituyó un espacio de intercambio y reflexión cuya realización ha sido posible gracias a la colaboración entre Facultades de Arquitectura, Urbanismo y Diseño de la Universidad Nacional y la Facultad de Arquitectura de la Universidad Católica de Córdoba, contando además con la activa participación de mayoría de las Facultades, Centros e Institutos de Historia de la Arquitectura del país y la región. Orientado en su convocatoria tanto a docentes como a estudiantes de Arquitectura y Diseño Industrial de todos los niveles de la FAUD-UNC promovió el debate de ideas a partir de experiencias concretas en instancias tales como mesas temáticas de carácter interdisciplinario, que adoptaron la modalidad de presentación de ponencias, entre otras actividades. En el ámbito de VIII Encuentro, desarrollado en la sede Ciudad Universitaria de Córdoba, se desplegaron numerosas posiciones sobre la enseñanza, la investigación y la formación en historia, teoría y crítica del diseño, la arquitectura y la ciudad; sumándose el aporte realizado a través de sus respectivas conferencias de Ana Clarisa Agüero, Bibiana Cicutti, Fernando Aliata y Alberto Petrina. El conjunto de ponencias que se publican en este Repositorio de la UNC son el resultado de dos intensas jornadas de exposiciones, cuyos contenidos han posibilitado actualizar viejos dilemas y promover nuevos debates. El evento recibió el apoyo de las autoridades de la FAUD-UNC, en especial de la Secretaría de Investigación y de la Biblioteca de nuestra casa, como así también de la Facultad de Arquitectura de la UCC; va para todos ellos un especial agradecimiento

COVID-19 symptoms at hospital admission vary with age and sex: results from the ISARIC prospective multinational observational study

Background: The ISARIC prospective multinational observational study is the largest cohort of hospitalized patients with COVID-19. We present relationships of age, sex, and nationality to presenting symptoms. Methods: International, prospective observational study of 60 109 hospitalized symptomatic patients with laboratory-confirmed COVID-19 recruited from 43 countries between 30 January and 3 August 2020. Logistic regression was performed to evaluate relationships of age and sex to published COVID-19 case definitions and the most commonly reported symptoms. Results: ‘Typical’ symptoms of fever (69%), cough (68%) and shortness of breath (66%) were the most commonly reported. 92% of patients experienced at least one of these. Prevalence of typical symptoms was greatest in 30- to 60-year-olds (respectively 80, 79, 69%; at least one 95%). They were reported less frequently in children (≤ 18 years: 69, 48, 23; 85%), older adults (≥ 70 years: 61, 62, 65; 90%), and women (66, 66, 64; 90%; vs. men 71, 70, 67; 93%, each P < 0.001). The most common atypical presentations under 60 years of age were nausea and vomiting and abdominal pain, and over 60 years was confusion. Regression models showed significant differences in symptoms with sex, age and country. Interpretation: This international collaboration has allowed us to report reliable symptom data from the largest cohort of patients admitted to hospital with COVID-19. Adults over 60 and children admitted to hospital with COVID-19 are less likely to present with typical symptoms. Nausea and vomiting are common atypical presentations under 30 years. Confusion is a frequent atypical presentation of COVID-19 in adults over 60 years. Women are less likely to experience typical symptoms than men

Técnicas innovadoras de producción de los híbridos de espárrago italianos : H668, Marte, UC 157, Giove, Semiforzado y acondicionamiento a diferentes largos

Resumen: El espárrago constituye una alternativa productiva que requiere de la optimización del manejo en los distintos eslabones de la cadena agroalimentaria, como así también de estrategias para la extensión del calendario de oferta. El objetivo de este trabajo fue evaluar algunas estrategias tendientes a la optimización de la productividad de la cadena espárrago. Se evaluó la productividad neta (PFN), número de turiones comerciales producidos (NT) y distribución de calibres (jumbo, extra large, large, medium, small y asparagina), en invernadero de los genotipos masculinos italianos: H668, Marte y Giove y el testigo americano UC157. Comparar su comportamiento en poscosecha, evaluando la evolución del peso fresco, mediante el empleo de diferentes alternativas de valorización: largos de los turiones (22 y 12 cm) y presentaciones (ACF: atados con film y ASF: atados sin film); considerando un peso inicial de 500 g. Se efectuó un análisis multifactor Anova LSD test. En PFN fue obtenido: ‘Giove’ 5.096,5 kg ha-1, ‘UC157’ 5.062,5 kg ha-1, ‘H668’ 3.187,5 y ‘Marte’ 2.043,5 kg ha-1. En NT se destacaron: ‘UC157’ 322.300 kg ha-1, ‘Giove’ 267.500 kg ha-1, ‘H668’ 240.000 kg ha-1 y ‘Marte’ 166.250 kg ha-1. En poscosecha, el uso de film permitió conservar mejor los turiones procesados, demostrado por la menor disminución del peso fresco: ACF 201,73 g y ASF 299,21 g. ‘Giove’ y ‘UC157’ presentaron mejor comportamiento,conservando 264,82 y 267,25 g, respectivamente. Los turiones cortos, acondicionados en atados,perdieron mayor peso fresco que los largos durante el periodo de poscosecha (22 cm: 228,13 g y 12 cm: 272,81 g

Accuracy of Tests for Diagnosis of Animal Tuberculosis: Moving Away from the Golden Calf (and towards Bayesian Models)

Authors’ Contributions AP and JA conceived and designed the study. AGB and JA conducted the screening of abstracts, and AGB, PP, and CPR the assessment of the full texts. AGB analyzed the data with the help and inputs from AB, AP, and JA. AGB and JA drafted the first version of the manuscript and received substantial feedback from all other authors.The last decades have seen major efforts to develop new and improved tools to maximize our ability to detect tuberculosis-infected animals and advance towards the objective of disease control and ultimately eradication. Nevertheless, there is still uncertainty regarding test performance due to the wide range of specificity and especially sensitivity estimates published in the scientific literature. Here, we performed a systematic review of the literature on studies that evaluated the performance of tuberculosis diagnostic tests used in animals through Bayesian Latent Class Models (BLCMs), which do not require the application of a (fallible) reference procedure to classify animals as infected with tuberculosis or not. BLCM-based sensitivity and specificity estimates deviated from those obtained using a reference procedure for certain antemortem tests: an overall lower sensitivity of skin tests and serology and a higher sensitivity of interferon-gamma (IFN-γ) assays was reported. In the case of postmortem diagnostic tests, sensitivity estimates from BLCMs were similar to estimates from studies based on other methodologies. For specificity, the range of BLCM-based estimates was narrower than those based on a reference test, reaching values close to 100% (but lower in the case of IFN-γ assays). In conclusion, Bayesian methods have been increasingly applied for the evaluation of tuberculosis diagnostic tests in animals, yielding results that differ (sometimes substantially) from previously reported test performance in the literature, particularly for in vivo tests and sensitivity estimates. Newly developed models that allow adjustment for relevant factors (e.g., age, breed, region, and herd size) can contribute to the generation of more unbiased estimates of test performance. Nevertheless, although BLCMs for tuberculosis do not require the use of an imperfect reference procedure and are therefore not influenced by its limited performance, they require careful implementation, and transparent systematic reporting should be the norm.Ministerio de Ciencia, Innovación y UniversidadesMinisterio de Economía y Competitividad (RTA2015-00043-C02-02; RYC-2016-20422)Depto. de Sanidad AnimalCentro de Vigilancia Sanitaria Veterinaria (VISAVET)TRUEpu

Productividad y calidad de cuatro híbridos de espárrago verde (Asparagus officinalis L. var. Altilis) en invernadero

Resumen: El espárrago constituye una alternativa productiva que requiere de la optimización del manejo en los distintos eslabones de la cadena agroalimentaria, como así también de estrategias para la extensión del calendario de oferta. El objetivo de este trabajo fue evaluar algunas estrategias tendientes a la optimización de la productividad de la cadena espárrago. Se evaluó la productividad neta (PFN), número de turiones comerciales producidos (NT) y distribución de calibres (jumbo, extra large, large, medium, small y asparagina), en invernadero de los genotipos masculinos italianos: H668, Marte y Giove y el testigo americano UC157. Comparar su comportamiento en poscosecha, evaluando la evolución del peso fresco, mediante el empleo de diferentes alternativas de valorización: largos de los turiones (22 y 12 cm) y presentaciones (ACF: atados con film y ASF: atados sin film); considerando un peso inicial de 500 g. Se efectuó un análisis multifactor Anova LSD test. En PFN fue obtenido: ‘Giove’ 5.096,5 kg ha-1, ‘UC157’ 5.062,5 kg ha-1, ‘H668’ 3.187,5 y ‘Marte’ 2.043,5 kg ha-1. En NT se destacaron: ‘UC157’ 322.300 kg ha-1, ‘Giove’ 267.500 kg ha-1, ‘H668’ 240.000 kg ha-1 y ‘Marte’ 166.250 kg ha-1. En poscosecha, el uso de film permitió conservar mejor los turiones procesados, demostrado por la menor disminución del peso fresco: ACF 201,73 g y ASF 299,21 g. ‘Giove’ y ‘UC157’ presentaron mejor comportamiento, conservando 264,82 y 267,25 g, respectivamente. Los turiones cortos, acondicionados en atados, perdieron mayor peso fresco que los largos durante el periodo de poscosecha (22 cm: 228,13 g y 12 cm: 272,81 g, de pérdida de peso, en relación a los 500 g iniciales, respectivamente). En síntesis, la productividad lograda, el anticipo y un adecuado acondicionamiento resultan alentadores para esta hortalizaAbstracts: Asparagus is an alternative crop that requires management optimization of the different links of the production chain as well as of the strategies to extend its supply year round. The objective of this paper was to evaluate some strategies to optimize the asparagus production chain. Net productivity (PFN), number of commercial turions produced (NT) and distribution of different sizes (jumbo, extra large, large, medium, small y asparagina) were evaluated in a greenhouse with Italian male genotypes: H668, Marte and Giove and the American control UC157. The post-harvest behavior was compared by evaluating the development of fresh weight by means of different evaluation alternatives: length of turions and presentation (ACF: bunch with film and ASF: bunch without film); with an initial weight of 500 g. A multifactor ANOVA LSD analysis test was carried out. For PFN: ‘Giove’ 5096.5 kg ha-1, ‘UC157’ 5062.5 kg ha-1, ‘H668’ 3187.5 kg ha-1, and ‘Marte’ 2,043.5 kg ha-1 were obtained. For NT: ‘UC157’ 322,300 kg ha-1, ‘Giove’ 267,500 kg ha-1, ‘H668’ 240,000 kg ha-1, and ‘Marte’ 166,250 kg ha-1 stood out. For post-harvest, the use of film allowed for better turion conservation, shown by the lower decrease of fresh weight: ACF 201.73 g and ASF 299.21 g. ‘Giove’ and ‘UC157’ performed better, conserving 264.82 and 267.25 g, respectively. Short turions lost more fresh weight (22 cm: 228.13 y 12 cm: 272.81 g, respectively). The attained productivity, the anticipated and adequate conditioning are encouraging for this vegetabl

Unlipidated Outer Membrane Protein Omp16 (U-Omp16) from Brucella spp. as Nasal Adjuvant Induces a Th1 Immune Response and Modulates the Th2 Allergic Response to Cow’s Milk Proteins

The discovery of novel mucosal adjuvants will help to develop new formulations to control infectious and allergic diseases. In this work we demonstrate that U-Omp16 from Brucella spp. delivered by the nasal route (i.n.) induced an inflammatory immune response in bronchoalveolar lavage (BAL) and lung tissues. Nasal co-administration of U-Omp16 with the model antigen (Ag) ovalbumin (OVA) increased the amount of Ag in lung tissues and induced OVA-specific systemic IgG and T helper (Th) 1 immune responses. The usefulness of U-Omp16 was also assessed in a mouse model of food allergy. U-Omp16 i.n. administration during sensitization ameliorated the hypersensitivity responses of sensitized mice upon oral exposure to Cow’s Milk Protein (CMP), decreased clinical signs, reduced anti-CMP IgE serum antibodies and modulated the Th2 response in favor of Th1 immunity. Thus, U-Omp16 could b

Inflammatory response induced after nasal delivery of U-Omp16.

<p>C57BL/6 mice (<i>n</i>=5/group) were intranasally administered with U-Omp16 or PBS as control and the number of total cells (<b>A</b>) and differential counts of macrophages (<b>B</b>), neutrophils (<b>C</b>) and lymphocytes (<b>D</b>) were determined in BAL at different time points. (<b>E</b>) Animals were intranasally administered with OVA_(AF647) alone or OVA_(AF647) plus U-Omp16. Lungs were obtained at different time points after delivery and the emission of fluorescence was evaluated in cell suspensions from each lung (1 x10⁶ cells). (<b>F</b>) C57BL/6 animals were administered through the nasal route with U-Omp16 or PBS as control and at different times post administration the level of TNF-α, IL-10 in lung tissues, and (<b>G</b>) TNF-α, IL-10, CCL2, CCL3 and CCL5 in BAL were determined by ELISA. Samples were assayed in duplicated and data represent the mean ±SEM from each group of five mice, (**<i>P</i><0.001, *<i>P</i><0.05 <i>vs</i> PBS group). These results are representative of 3 independent experiments with similar results.</p