MARICULTURE DEVELOPMENT IN INDONESIA: Prospects and Constraints

Mariculture is an important component of Indonesian fisheries and aquaculture production, directly contributing an estimated US$ 320 million in 2008. Because most mariculture production is focussed on producing for export markets, mariculture production is an important source of foreign earnings for the Indonesian economy. This paper reviews the current status and prospects for continuing development of mariculture in Indonesia. Currently the major mariculture commodity in Indonesia is seaweed for carrageenan production. Seaweed production accounts for 98% of total Indonesian mariculture production and 84% of value. The other major commodity groups are marine finfish and pearl oysters. Commodities being developed for mariculture in Indonesia include abalone and spiny lobsters. Prospects for continued development of mariculture in Indonesia appear positive. Indonesia has several advantages for mariculture development, including many potential mariculture sites, a stable tropical climate, and does not suffer from cyclonic storms. The Government of Indonesia is planning to increase aquaculture production substantially over the next four years, including mariculture production. Globally, demand for seafood products is expanding due to increasing population and increased per capita consumption of fish products. Constraints to the continued development of mariculture in Indonesia include: limited seed supply, particularly of species which cannot be economically produced in hatcheries, such as spiny lobsters; need to develop more efficient production systems for some marine finfish; the need to improve environmental sustainability by improving feeds and reducing environmental impacts; and market issues relating to environmental sustainability

The Transformative Impact of Extracellular Vesicles on Developing Sperm

Objective: To review the role of extracellular vesicles (EVs) released from the male reproductive tract and their impact on developing sperm. We discuss how sperm exiting the seminiferous tubules, although developmentally mature, require further modification. Acquisition of various functions including increased motility, transfer of cargoes and ability to undertake the acrosome reaction is mediated through the interaction between sperm and EVs. Methods: A review of the literature identified that EVs are released from different portions of the male reproductive tract, notably the epididymis and prostate. These EVs interact with sperm as they pass from the seminiferous tubules to the epididymis and vas deferens prior to ejaculation. Results: EVs are small lipid-bound particles carrying bespoke RNA, protein and lipid cargoes. These cargoes are loaded based on the state of the parent cell and are used to communicate with recipient cells. In sperm, these cargoes are essential for post-testicular modification. Conclusions: Interactions between developing sperm and EVs are important for the subsequent function of sperm. Prior to ejaculation, these interactions confer important changes for the post-testicular modification and development of sperm. Little is known about the interaction between EVs from the testes and the spermatogonial stem cell niche or developing sperm within the seminiferous tubules. However, the numerous roles of EVs in the post-testicular modification of sperm have led many to suspect that they may also play important roles in developing sperm within the testes

Moral theory of Thomas Traherne, with special attention to the pro-formative role of nature in the moral formation of children

In the mid seventeenth century, Thomas Traherne claimed human beings must retire into creation in order to fully know the virtues, including goodness, peaceableness and care. In this thesis I review Traherne's moral theory in light of recently discovered manuscripts of his work. For Traherne, because God's Divine goodness is the efficient cause of creation, creatures are naturally good. He uses Paracelsian optic and atomic theories to indicate how creatures communicate their goodness to one another. By retiring among creatures in their natural place, he argues that persons create a relational theatre in which they develop their capacity to sense creaturely communication. In this 'communion' persons perceive their mutual 'interest' with creatures in the relational nexus of creation. This knowledge provides motivation for 'blessed operations' of care for persons and creation. Because the human relationship to other creatures is morally significant, retiring among creation is a critical part of Christian moral formation. For Traherne this sensual engagement with a relational creation is necessary in the moral formation of children, who apprehend nature with their senses. Their innate wonder equips them to form their moral identity in relationship to a peaceable, caring creation. Traherne's account of the role of nature in moral development raises significant pedagogical questions in an age when scientific knowledge and the senses were increasingly disassociated from moral reasoning. For Traherne an education that denies the role of the senses in moral formation 'murders' the child by distracting her attention from the virtues of peace and mutuality that are present in creation. In conversation with phenomenologist Maurice Merleau-Ponty, child psychologists Colwyn Trevarthen and Darcia Narvaez, and educational philosophers David Carr and Carol Gilligan, this thesis demonstrates the contemporary significance of Traherne's claims. Through the wonder of play, contact with the natural environment helps children develop an 'ecological' identity based on their relationship to other creatures. The perception of care in these early relationships is the basis for forming an inter-subjective moral identity and the virtues of care. Many 'care' ethicists and psychologists emphasize the early experiences of care taking. Environmental educators emphasize the caring relationship to creation. Hence they give substance to Traherne's claim that play, wonder and a sensory relationship with other creatures at an early age contribute to the formation of moral identity. Traherne's ideas also have pedagogical implications for theories of Christian moral formation. Theologians and ethicists, such as Rowan Williams, Michael Northcott and John Inge, have suggested place-based programmes of moral formation are needed in the parish context. This thesis demonstrates that Traherne's moral theory provides a rationale for understanding the theological significance of a child's natural wonder and the need for its cultivation in programmes of Christian education. A relationship to the local ecology of the parish can help a child perceive the care of creation, and play a proformative role in developing a moral identity in relationship to a caring Creator

Extracellular vesicles in urological malignancies

Extracellular vesicles (EVs) are small lipid bound structures released from cells containing bioactive cargoes. Both the type of cargo and amount loaded varies compared to that of the parent cell. The characterisation of EVs in cancers of the male urogenital tract has identified several cargoes with promising diagnostic and disease monitoring potential. EVs released by cancers of the male urogenital tract promote cell-to-cell communication, migration, cancer progression and manipulate the immune system promoting metastasis by evading the immune response. Their use as diagnostic biomarkers represents a new area of screening and disease detection, potentially reducing the need for invasive biopsies. Many validated EV cargoes have been found to have superior sensitivity and specificity than current diagnostic tools currently in use. The use of EVs to improve disease monitoring and develop novel therapeutics will enable clinicians to individualise patient management in the exciting era of personalised medicine

Organic Geochemical and Petrographic Analysis of Pure Macerals from the Ohio Shale

Recent advances now permit the separation of coal into constituent macerals of high purity using density gradient centrifugation (DGC). With the availability of pure macerals, the chemical structure of each can be investigated separately, without the interactive interference of the other macerals or mineral matter. The present study is a preliminary effort applying these methods to the study of oil shale kerogen. We have used a sample from the Huron Member of the Upper Devonian Ohio Shale from Logan County, Ohio. Whole rock petrographic examination revealed intact Tasmanites, telalginite of unknown derivation and a weakly fluorescing matrix. Pyrite is abundant, including framboidal and euhedral pyrite imbedded within macerals. The kerogen concentrate was subjected to DGC. The resulting profile shows a single, broad main peak, consisting of mixed telaginite, amorphinite and rare vitrinite. Variable amounts of entrapped minerals apparently account for the broad range of density within the peak, with the organic assemblage being fairly consistent. In future work, it is recommended that the kerogen be micronized prior to DOC to permit a cleaner separation. In order to investigate the chemistry of macerals, pyrolysis is preferred over simple extraction, since soluble native bitumen is mobile and may migrate from the maceral of origin into a neighboring one, acting as a natural contaminant. Pyrolysis techniques assure that the data reflect the nature of indigenous material only. The Ohio Shale kerogen and 4 DGC fractions were subjected to micro-scale, anhydrous, in vitro pyrolysis, followed by GCMS of the saturate and aromatic LC fractions of the pyrolyzate. The 4 DGC fractions are nearly identical in both their saturate and aromatic molecular distributions, consistent with the petrographic observations. To further demonstrate the efficacy of the method, a pure sporinite isolated from a coal in the Pennsylvanian Brazil Formation of Indiana was also analyzed. The sporinite pyrolyzate can readily be distinguished from that of the Ohio Shale alginite by the distributions of n-alkanes, isoprenoids, phyllocladane derivatives, extended tricyclic terpanes, hopanes, moretanes, steranes, alkylbenzenes and thiophene derivatives. Py-LC-GCMS is an effective and versatile characterization tool, as it provides a great number of molecular parameters

Impact of taxane-based chemotherapeutics on male reproductive function

Men and boys with cancer treated with chemotherapy are known to have reduced fertility following their treatment. This is because some chemotherapy drugs can damage the cells in the testicles that make sperm. This study found there is limited information available on the effect of one group of chemotherapy drugs, called taxanes, on testicular function and fertility. More studies are needed to aid clinicians in advising patients on how this taxane-based chemotherapy may affect their future fertility

Improved hatchery and grow-out technology for marine finfish aquaculture in the Asia–Pacific region

This project focussed on improving marine finfish aquaculture production in the Asia-Pacific region by focussing on key constraints: improving hatchery technology to improve the availability of seedstock; evaluating the nutritional needs of groupers to support the development of compounded pellet diets; and improving communication and coordination of marine finfish aquaculture research and development activities in the Asia-Pacific region

Cisplatin effects on the human fetal testis - establishing the sensitive period for (pre)spermatogonial loss and relevance for fertility preservation in pre-pubertal boys

BACKGROUND: Exposure to chemotherapy during childhood can impair future fertility. Studies using in vitro culture have shown exposure to platinum-based alkylating-like chemotherapy reduces the germ cell number in the human fetal testicular tissues. We aimed to determine whether effects of exposure to cisplatin on the germ cell sub-populations are dependent on the gestational age of the fetus and what impact this might have on the utility of using human fetal testis cultures to model chemotherapy exposure in childhood testis. METHODS: We utilised an in vitro culture system to culture pieces of human fetal testicular tissues (total n=23 fetuses) from three different gestational age groups (14-16 (early), 17-19 (mid) and 20-22 (late) gestational weeks; GW) of the second trimester. Tissues were exposed to cisplatin or vehicle control for 24 hours, analysing the tissues 72 and 240 hours post-exposure. Number of germ cells and their sub-populations, including gonocytes and (pre)spermatogonia, were quantified. RESULTS: Total germ cell number and number of both germ cell sub-populations were unchanged at 72 hours post-exposure to cisplatin in the testicular tissues from fetuses of the early (14-16 GW) and late (20-22 GW) second trimester. In the testicular tissues from fetuses of mid (17-19 GW) second trimester, total germ cell and gonocyte number were significantly reduced, whilst (pre)spermatogonial number was unchanged. At 240 hours post-exposure, the total number of germ cells and that of both sub-populations was significantly reduced in the testicular tissues from fetuses of mid- and late-second trimester, whilst germ cells in early-second trimester tissues were unchanged at this time-point. CONCLUSIONS: In vitro culture of human fetal testicular tissues can be a useful model system to investigate the effects of chemotherapy-exposure on germ cell sub-populations during pre-puberty. Interpretation of the results of such studies in terms of relevance to later (infant and pre-pubertal) developmental stages should take into account the changes in germ cell composition and periods of germ cell sensitivity in the human fetal testis

Diversification of brackishwater aquaculture in Indonesia: tilapia culture in Aceh

Traditional brackishwater aquaculture in Indonesia focuses on production of shrimp and milkfish. However, since the 1990’s production of shrimp, particularly Penaeus monodon, has become problematic because ofthe prevalence of white-spot disease. Crop losses due to white-spot disease have limited production of P. monodon in traditional tambak throughout Indonesia. Because of this, many tambak have fallen into disuse, or their use islimited. In response, we are developing and trialling diversification options for tambak farmers in Aceh and South Sulawesi. Alternative production options that we are evaluating include Nile tilapia (ikan nila) and soft-shell crab(kepiting lunak) in Aceh, and Nile tilapia, swimming crab (rajungan) and rabbitfish (baronang) in South Sulawesi. In Aceh our main focus has been on Nile tilapia (Oreochromis niloticus) culture, either alone or co-cultured with milkfish (Chanos chanos). We have undertaken trials with farmers in Samalanga and Jangka (Kab. Bireuen) and Banda Mulia (Kab. Aceh Tamiang). Overall, our trials have shown that tilapia will grow and survive well at salinities below 20 ppt. Above 20 ppt, growth rate is reduced and the fish are prone to bacterial disease. At salinities above 20 ppt, milkfish (bandeng) perform better in ponds than tilapia. Our trials in Aceh have demonstrated that Nile tilapia can provideincome to farmers comparable with shrimp culture. Out of a total of 22 trials to date, 9 ponds (41%) have met or exceeded our reference profitability of IDR 1–5 million per hectare per crop. Fourteen ponds (64%) have been‘profitable’, i.e. have generated positive economic returns. This figure is similar to the profitability figure for traditional shrimp ponds (64–73%), indicating that Nile tilapia culture in brackishwater ponds provides economic returns similar to traditional shrimp culture. Based on these results, we recommend Nile tilapia culture to farmers whose ponds are 20 ppt. This provides a profitable production option for farmers in areas subject to low salinities, or during the rainy season when salinities are depressed. Because outbreaks of white-spot disease are particularly common and severe during the rainy season, Nile tilapia culture provides a viable option for income generation for Acehnese tambak farmers under conditions where shrimp culture is too risk