Increased expression of aggrecan and biglycan mRNA in Achilles tendinopathy

To determine the expression of mRNA encoding the proteoglycans aggrecan, versican, biglycan and decorin in mid-tendon samples of chronic painful Achilles tendinopathy and ruptured Achilles tendons, compared with normal tendons. Total RNA isolated from frozen tendon samples (14 normal, 13 painful, 14 ruptured) was assayed by relative quantitative reverse transcription polymerase chain reaction for aggrecan, versican, biglycan and decorin mRNA, normalized using 18S rRNA. Differences between sample groups were tested by univariate analysis of variance with age as co-variate. In normal tendon samples expression of each of the proteoglycan mRNA decreased with increasing age. Decorin mRNA was the most highly-expressed of the proteoglycan mRNA, while versican mRNA expression was higher (3.8-fold) than that of aggrecan. In painful tendinopathy both aggrecan and biglycan mRNA expression increased (more than 10-fold and 5-fold, respectively) compared with normal tendon samples, but levels of versican and decorin mRNA were not significantly changed. In ruptured tendons the levels of aggrecan, biglycan and versican mRNA were not changed compared with normal tendon samples, but decorin mRNA decreased markedly. Increased aggrecan and biglycan mRNA expression in painful tendinopathy resembles the pattern in fibrocartilaginous regions of tendon, and may reflect an altered mechanical environment at the site of the lesion. Increased aggrecan mRNA expression may underlie the increase in glycosaminoglycan observed in painful tendinopathy

Versican splice variant messenger RNA expression in normal human Achilles tendon and tendinopathies

Versican is the principal large proteoglycan expressed in mid-tendon, but its role in tendon pathology is unknown. Our objective was to define the expression of versican isoform splice variant messenger ribonucleic acid (mRNA) in normal Achilles tendons, in chronic painful tendinopathy and in ruptured tendons. Total RNA isolated from frozen tendon samples (normal n = 14; chronic painful tendinopathy n = 10; ruptured n = 8) was assayed by relative quantitative reverse transcriptase polymerase chain reaction (RT-PCR) for total versican, versican variants V0, V1, V2, V3 and type I collagen a1 mRNA, normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Differences between sample groups were tested by Wilcoxon statistics. Painful and ruptured tendons showed a significant decrease (median 2-fold) in the expression of versican mRNA, in contrast to an increased expression (median 8-fold) of type I collagen a1 mRNA in painful tendons. Versican splice variants V0 and V1 mRNA were readily detected in normal samples, V3 levels were substantially lower, and V2 levels were more variable. Each of V1, V2 and V3 mRNA showed significant decreases in expression in painful and ruptured tendons, but V0 was not significantly changed. Changes in versican expression relative to that of collagen, and alterations in the balance of versican splice variants, may contribute to changes in matrix structure and function in tendinopathies

Prevalence of POE in UK Higher Education Institutions

Purpose: To identify the extent to which POE is understood and applied within higher education institutions across the UK and to identify users' perceptions of its efficacy. Design/Methodology: A mixed methodology was applied to this research. Part one consisted of an online survey intended to generate an overview of the extent of POE use across the sector. The second phase, intended to develop greater insight and understanding of users' perceptions of POE, relied upon in-depth interviews with a smaller sample drawn those who had completed the survey in part one. Findings: Differing models POE are used within higher education institutions with varying degrees of complexity. Models exist that are based on standard structures and templates, whilst entirely bespoke options have been developed by some institutions. The work found that there is a clear preference for applying bespoke methods rather than standardised ones. Research limitations/implications: The research is based on a sample of Directors of Estates from UK Universities. The conclusions relate specifically to that sector and context. The extent for broader generalisation is limited by the research sample. Practical implications: The work shows that POE is understood to have differing purpose and effectiveness in individual Universities. This leads to the conclusion that more coherent and consistent understanding should be promoted within the sector. Social Implications: The work has implications for the effective management of university buildings to support user satisfaction. Originality/Value: This paper indicates that there is prevalence of applying POE processes in higher education institutions and that specific models have been developed for the sector. However, the extent to which the process is valued and understood is highly variable

Older People’s Preferences for Housing and Environment Characteristics

Population ageing presents significant challenges for many countries, one of which is the provision of adequate housing. Developing understanding of the needs and preferences of ageing societies will be crucial in order to assist in the provision of suitable housing and communities that are sustainable in the long term. While a preference to ‘age in place’ is clear in the literature, comparatively less academic research is available on older people’s preferences for more specific housing and environment attributes. The aim of this study is to identify the main housing and environment characteristics that are linked to the health and wellbeing of the elderly and determine the preferences for such characteristics via a survey with UK residents aged 55+. The results indicate a strong preference for independent living and an increasing desire for bungalows in later life. Housing conditions, energy efficiency, thermal comfort, and home adaptions to facilitate ageing in place are particularly important housing characteristics to older people. The location and environment are also key drivers of housing preferences; a safe neighbourhood, accessibility to amenities, public transport, and a clean and walkable environment are particularly important. Preferences varied with age, but gender has a less significant impact on the preferences expressed. The findings of this study will be valuable for stakeholders engaged in housing policy and provision for older people

The Effect of Indoor Environment towards Middle-Income Group in Re-Engineered Affordable Housing Scheme in Malaysia

The innovation of housing has evolved due to several issues such as the affordability and the sustainability of housing. Several housing designs were introduced to meet the affordable housing standard price for the middle-income group, particularly in the urban area. However, it is a challenge for the developers to provide affordable housing within the current market price with the element of sustainability. The developers use the concept of high rise building form and located near the public amenities accommodate the users’ needs as well as to gain profits. Despite the rising demands, there is a limited study that focuses on the performance of the buildings, specifically on the indoor environmental quality (IEQ) of the affordable housing. This paper aims to assess the affordable housing performance by identifying the residents’ satisfaction level towards IEQ and the importance of the IEQ factors as well as their health condition. A questionnaire survey is one of the methods used in this study. The findings showed that the IEQ of affordable housing in Malaysia has resulted in the decrease of health towards the residents, with some dissatisfaction towards the IEQ. The insight from the residents can be used as an indicator to improve the IEQ as well as the building performance and to create a better affordable housing in Malaysia

Residents' Satisfaction Towards the Indoor Environmental Quality of Re-engineered Affordable Housing Scheme in Malaysia

Malaysia is currently focusing on providing affordable housing all over the country, as part of the plan to tackle the problem of the rising costs of house ownership due to reasons such as the economic crisis and land limitations. However, a limited number of methodical studies have been conducted on residents’ feedback on the indoor environment of affordable housing. Indoor Environmental Quality (IEQ) is an important aspect of occupants’ wellbeing, as it affects their health and productivity. Therefore, evaluating residents’ feedback on IEQ is an important step in gauging building performance and conditions. In addition, the health and productivity of residents needs to be evaluated in order to identify the effect of IEQ on them. A questionnaire survey was used to achieve the study objectives and to gain access to the respondents’ views and feedback. The pilot questionnaire study was conducted at two housing apartments in Kuala Lumpur, Malaysia, and the data collected were evaluated and analyzed using SPSS software. The outcome of this pilot study is significant, as it shows the residents’ dissatisfaction level towards IEQ factors such as noise, glare and privacy, and other aspects of IEQ that are important, such as ventilation. Additionally, it has been found that the IEQ of the residents does affect their health and productivity. It is anticipated that the outcome of this study will serve as an indicator of building performance improvements needed to achieve a better indoor environment quality for affordable housing in Malaysia

Determining Significant Parameters on Health and Well-Being of Building Occupants Towards Re-Engineered Inclusive Environment

People spend over 90% of their time in the buildings. Building is a place where people are spending more time doing activities, thus an inclusive environment of the buildings is very important to ensure that people are having a healthy life. Inclusive environment is important for the well-being of building occupants. Indoor environmental quality is one of the essential elements in creating inclusive environment in which a poor quality of indoor environmental affects the health of the occupants both physically and mentally, their performance, productivity, comfort, satisfaction, and well-being. Even though people are towards creating inclusive environment but it is limited study on the parameters of the inclusive environment. Thus, this paper is to study the parameters of inclusive environment by focused on the element of indoor environmental quality for building occupants based on literature reviews of articles between the year 2006 and 2016. Based on the content analysis, it has been discovered that there are various parameters of inclusive environment which are visual comfort, thermal comfort, acoustic comfort, indoor air quality, buildings factors, occupants’ factors, and climate condition factors. The parameters can be very useful as guidelines and development of policy in providing inclusive environment for the healthy lifestyle of building occupants

10C continued: A deeper radio survey at 15.7 GHz

We present deep 15.7-GHz observations made with the Arcminute Microkelvin Imager Large Array in two fields previously observed as part of the Tenth Cambridge (10C) survey. These observations allow the source counts to be calculated down to 0.1 mJy, a factor of five deeper than achieved by the 10C survey. The new source counts are consistent with the extrapolated fit to the 10C source count, and display no evidence for either steepening or flattening of the counts. There is thus no evidence for the emergence of a significant new population of sources (e.g. starforming) at 15.7 GHz flux densities above 0.1 mJy, the flux density level at which we expect starforming galaxies to begin to contribute. Comparisons with the de Zotti et al. model and the SKADS Simulated Sky show that they both underestimate the observed number of sources by a factor of two at this flux density level. We suggest that this is due to the flat-spectrum cores of radio galaxies contributing more significantly to the counts than predicted by the models.We thank the staff of the Mullard Radio Astronomy Observatory for maintaining and operating AMI. IHW and CR acknowledge Science and Technology Facilities Council studentships. IHW acknowledges support from the Square Kilometre Array South Africa project and the South African National Research Foundation. This research has made use of NASA’s Astrophysics Data System. We thank the referee for their careful reading of this manuscript.This is the final version of the article. It first appeared from Oxford University Press via http://dx.doi.org/10.1093/mnras/stv296

Efficacy of an ankle orthosis with a subtalar locking system in restricting ankle kinetics and kinematics in lateral cutting

Introduction The ankle joint is the most injured joint during sports participation [1]. Ankle orthoses have been shown to be effective in reducing ankle inversion injuries and are often prescribed for rehabilitation and prevention of lateral ankle sprains. Efficacy of ankle orthoses is often assessed by comparing reduction of passive inversion ROM as well as ankle kinematics between braced and unbraced movements [2,3]. However, joint kinetic responses in lateral cutting were rarely examined. Therefore, the objective of this study was to examine the effectiveness of a new semi-rigid ankle orthosis with a subtalar joint locking mechanism in restricting ankle kinetics and kinematics during a lateral cutting movement

High-Resolution Magnetic Resonance Imaging of the Regenerating Adult Zebrafish Heart

The adult zebrafish is a well-established model for studying heart regeneration, but due to its tissue opaqueness, repair has been primarily assessed using destructive histology, precluding repeated investigations of the same animal. We present a high-resolution, non-invasive in vivo magnetic resonance imaging (MRI) method incorporating a miniature respiratory and anaesthetic perfusion set-up for live adult zebrafish, allowing for visualization of scar formation and heart regeneration in the same animal over time at an isotropic 31 µm voxel resolution. To test the method, we compared well and poorly healing cardiac ventricles using a transgenic fish model that exhibits heat-shock (HS) inducible impaired heart regeneration. HS-treated groups revealed persistent scar tissue for 10 weeks, while control groups were healed after 4 weeks. Application of the advanced MRI technique allowed clear discrimination of levels of repair following cryo- and resection injury for several months. It further provides a novel tool for in vivo time-lapse imaging of adult fish for non-cardiac studies, as the method can be readily applied to image wound healing in other injured or diseased tissues, or to monitor tissue changes over time, thus expanding the range of questions that can be addressed in adult zebrafish and other small aquatic species