Disquinesia ciliar primaria en un bichon Frisé macho de un año de edad

Se describe el caso clínico de un Bichon Frisé macho de un año de edad, con signos respiratorios desde los dos meses de vida. El motivo de la consulta fue la presentación de un cuadro de tos crónica productiva de dos meses de duración y posibles episodios recurrentes de rinosinusitis. El perro había sido tratado anteriormente con antibióticos obteniéndose una respuesta parcial. La historia clínica, el examen radiográfico, ecocardiográfico y de ECG ayudaron a destacar otros problemas respiratorios y cardíacos para llegar a la sospecha de una disquinesia ciliar primaria. Se procedió al examen ultraestructural con el microscopio electrónico de trasmisión de cilios de la mucosa nasal y de los flagelos de los espermatozoides. La muestra de la mucosa nasal quedó invalidada por la presencia de eritrocitos pero se pudo llegar al diagnóstico a través de las alteraciones características encontradas en los brazos de dineína y en los microtúbulos de los flagelos de los espermatozoides. El tratamiento de esta enfermedad hereditaria es de por vida se basa en el control de las infecciones respiratorias recidivantes con una antibioterapia específica mediante cultivos previos del exudado nasal o lavado traqueal. El pronóstico es reservado y depende del control de estas infecciones secundarias. Aunque esta enfermedad suele conllevar una infertilidad o subfertilidad del macho, el animal debe retirarse de la reproducción. Palabras clave: Disquinesia ciliar primaria; Examen ultraestrctural; Espermatozoides

Evaluation of the Probiotic In Vitro Potential of Lactic Acid-Producing Bacteria from Canine Vagina : possible Role in Vaginal Health

Lactic acid-producing bacteria (LAB) are being widely studied due to their probiotic potential. The aim of the present study was to determine and identify the presence of LAB from canine vaginal samples, as well as to evaluate their probiotic in vitro potential. Ninety-four bitches were included in the study. Vaginal samples were obtained by means of a sterile swab and streaked on Man Rogosa Sharpe agar plates. A total of 100 LAB strains were obtained and submitted to Gram stains and basic biochemical tests, which included catalase, oxidase and haemolysis tests. Thirteen strains belonging to the genera Lactobacillus (n = 10), Lactococcus (n = 2) and Pediococcus (n = 1) were selected as potential probiotics and further subjected to evaluation of resistance to gastrointestinal conditions (pH, lysozyme, bile salts and hydrogen peroxide) and safety and efficacy in vitro (resistance to antibiotics and antimicrobial capacity). Only three strains, one Lactobacillus lactis and two Lactobacillus plantarum, accomplished the requirements for being considered as potential in vitro probiotics

Disquinesia ciliar primaria en un bichon Frisé macho de un año de edad

Se describe el caso clínico de un Bichon Frisé macho de un año de edad, con signos respiratorios desde los dos meses de vida. El motivo de la consulta fue la presentación de un cuadro de tos crónica productiva de dos meses de duración y posibles episodios recurrentes de rinosinusitis. El perro había sido tratado anteriormente con antibióticos obteniéndose una respuesta parcial. La historia clínica, el examen radiográfico, ecocardiográfico y de ECG ayudaron a destacar otros problemas respiratorios y cardíacos para llegar a la sospecha de una disquinesia ciliar primaria. Se procedió al examen ultraestructural con el microscopio electrónico de trasmisión de cilios de la mucosa nasal y de los flagelos de los espermatozoides. La muestra de la mucosa nasal quedó invalidada por la presencia de eritrocitos pero se pudo llegar al diagnóstico a través de las alteraciones características encontradas en los brazos de dineína y en los microtúbulos de los flagelos de los espermatozoides. El tratamiento de esta enfermedad hereditaria es de por vida se basa en el control de las infecciones respiratorias recidivantes con una antibioterapia específica mediante cultivos previos del exudado nasal o lavado traqueal. El pronóstico es reservado y depende del control de estas infecciones secundarias. Aunque esta enfermedad suele conllevar una infertilidad o subfertilidad del macho, el animal debe retirarse de la reproducción. Palabras clave: Disquinesia ciliar primaria; Examen ultraestrctural; Espermatozoides

The presence of a high-Km hexokinase activity in dog, but not in boar, sperm

The presence of a high-Km hexokinase activity was tested in both dog and boar spermatozoa. Hexokinase kinetics from dog extracts showed the presence of a specific activity (dog-sperm glucokinase-like protein, DSGLP), in the range of glucose concentrations of 4-10 mM, whereas boar sperm did not show any DSGLP activity. Furthermore, dog-sperm cells, but not those of boar, showed the presence of a protein which specifically reacted against a rat-liver anti-glucokinase antibody. This protein also had a molecular weight equal to that observed in rat-liver extracts, suggesting a close similarity between both the proteins. This glucokinase-like protein was distributed in the peri- and post-acrosomal zones of the head, and the midpiece and principal piece of tail of dog spermatozoa. These results indicate that dog spermatozoa have functional high-Km hexokinase activity, which could contribute to a very fine regulation of their hexose metabolism. This strict regulation could ultimately be very important in optimizing dog-sperm function along its life-time

Rat age-related benign prostate hyperplasia is concomitant with an increase in the secretion of low ramified α-glycosydic polysaccharides

Acord transformatiu CRUE-CSICWe would like to thank Dr. Manel Bosch (Advanced Optical Microscopy Facilities, University of Barcelona) for his invaluable advice and collaboration to obtain microscope images of both Haematoxylin-Eosin-stained samples and PAS ones. No disclosure was applicable on this work. Likewise, there is no conflict of interest related with this manuscript. Furthermore, no specific Funding was afforded for this work.This work analysed the expression of prostate polysaccharides in rats with age-related benign prostatic hyperplasia (BPH) for a better understanding of the possible relationship between prostate polysaccharides secretion and BPH onset. For this, prostatic glands from 1 month-old, 3 months-old, 6 months-old and 12 months-old Sprague-Dawley rats were processed in order to identify their overall polysaccharide content. Additionally, serum testosterone was also determined. One-month old rats showed significantly (P < 0.05) lower testosterone levels (0.77 ng/mL±0.12 ng/mL) compared with the other groups, which showed no significant difference among them. PAS staining showed positive polysaccharides markings in both the prostatic lumen and inside of luminal prostatic cells in all groups. Semiquantitative analysis of intraluminal PAS showed that one month-old rats had significantly (P < 0.005) lower PAS intensity when compared with all other groups (100.0 ± 0.5, arbitrary units vs. 107.3 ± 0.6, arbitrary units in 3 months-old ones), whereas 12 months-old ones showed significantly (P < 0.005) higher values when compared with all other groups (133.6 ± 3.5, arbitrary units in 12 months-old rats vs. 108.6 ± 1.4, arbitrary units in 6 months-old ones). The PAS + content practically disappeared when tissues were pre-incubated with either α-amylase or amyloglucosidase, regardless of a previous incubation with proteinase K. Incubation of prostate extracts from 12 months-old rats for 2 h with α-amylase yielded a significantly higher amount of free glucose (1.47 nmol/mg protein±0.23 nmol/mg protein vs. 0.32 nmol/mg protein±0.01 nmol/mg protein in untreated extracts). Similar results were obtained when extracts were pre-incubated with amyloglucosidase. Contrarily, pre-incubation with N-glycosidase induced a significantly (P < 0.05), much lower increase of free glucose. Pre-treatment with proteinase K did not significantly modify these results, which indicate that BPH is related to an increase in the secretion of low ramified ductal α-glycosydic polysaccharides that were not protected against lysis by any type of protein protective core. These changes seem to not be related with concomitant variations in serum testosterone levels

Cesària d'una vaca

Títol a la caràtula: Tècniques de cesària en vaque