Dendritic Ventriloquism: Inhibitory Synapses Throw Their Voices

In a theoretical study in this issue of Neuron, Gidon and Segev (2012) identify several new principles governing how inhibition interacts with excitation in active dendrites. They show that inhibitory synapses can interact with excitability at a distance, effectively “throwing their voices” in the dendritic tree, such that distributed inhibitory synapses can act synergistically to provide a global veto of dendritic excitability

Structured Connectivity in Cerebellar Inhibitory Networks

SummaryDefining the rules governing synaptic connectivity is key to formulating theories of neural circuit function. Interneurons can be connected by both electrical and chemical synapses, but the organization and interaction of these two complementary microcircuits is unknown. By recording from multiple molecular layer interneurons in the cerebellar cortex, we reveal specific, nonrandom connectivity patterns in both GABAergic chemical and electrical interneuron networks. Both networks contain clustered motifs and show specific overlap between them. Chemical connections exhibit a preference for transitive patterns, such as feedforward triplet motifs. This structured connectivity is supported by a characteristic spatial organization: transitivity of chemical connectivity is directed vertically in the sagittal plane, and electrical synapses appear strictly confined to the sagittal plane. The specific, highly structured connectivity rules suggest that these motifs are essential for the function of the cerebellar network

Measurements of Multi-Layer Insulation at High Boundary Temperature, using a Simple Non-Calorimetric Method

In spite of abundant literature, the thermal performance of Multi-Layer Insulation (MLI) still deserves dedicated investigation for specific applications, as the achievable insulation strongly depends on installation details. Furthermore, less accurate information is available for warm than for cold boundaries, since errors due to edge effects in small test benches increase strongly with warm boundary temperature. We establish here the thermal performance of MLI between 300 K and 77 K or 4 K, without bringing calorimetric methods into play, through the accurate measurement of a temperature profile. A cylinder in thin copper, wrapped with MLI, is cooled at one extremity while suspended under vacuum inside a sheath at room temperature. For known thermal conductivity and thickness of the tube, the heat flux can be inferred from the temperature profile. In-situ measurement of the thermal conductivity is obtained by applying a know heat flow at the warm extremity of the cylinder. Results, cross-checked with a calibrated heatmeter, compare well with what previously obtained on a large-scale measuring facility

A Microcryostat for Refrigeration at 1.8 K

A microcryostat has been developed in the Central Cryogenic Laboratory at CERN with the purpose of cooling a prototype beam loss monitor for the LHC, based on bolometry at 1.8 K. Its characteristics a re the very compact volume (some cm3 LHe) ensuring short cooldown-warmup times, and its low heat losses (~ 8 mW). The cryostat can be mounted on top of a small dewar through a rigid straight transfer line for continuous feeding

A Large-scale Test Facility for Heat Load Measurements down to 1.9 K

Laboratory-scale tests aimed at minimizing the thermal loads of the LHC magnet cryostat have gone along with the development of the various mechanical components. For final validation of the industrial design with respect to heat inleaks between large surfaces at different temperatures, a full-scale test cryostat has been constructed. The facility reproduces the same pattern of temperature levels as the LHC dipole cryostat, avoiding the heat inleaks from local components like supports and feedthroughs and carefully minimizing fringe effects due to the truncated geometry of the facility with respect to the LHC cryostats serial layout. Thermal loads to the actively cooled radiation screen, operated between 50 K and 65 K, are measured by enthalpy difference along its length. At 1.9 K, the loads are obtained from the temperature difference across a superfluid helium exchanger. On the beam screen, the electrical power needed to stabilize the temperature at 20 K yields a direct reading of the heat losses. Precise in-situ calibration is achieved by subcooling the thermal screen, thereby zeroing radiative heat loads. Minimizing fringe effects has been rewarded by a high precision measurement, yielding one of the more accurate quantifications to date of an industrial application of MLI. The influence of possible openings in the thermal screen is monitored both at the 1.9 K bath and with a radiation sensitive bolometer

Mutations in the LHX2 gene are not a frequent cause of micro/anophthalmia

Purpose: Microphthalmia and anophthalmia are at the severe end of the spectrum of abnormalities in ocular development. A few genes (orthodenticle homeobox 2 [OTX2], retina and anterior neural fold homeobox [RAX], SRY-box 2 [SOX2], CEH10 homeodomain-containing homolog [CHX10], and growth differentiation factor 6 [GDF6]) have been implicated mainly in isolated micro/anophthalmia but causative mutations of these genes explain less than a quarter of these developmental defects. The essential role of the LIM homeobox 2 (LHX2) transcription factor in early eye development has recently been documented. We postulated that mutations in this gene could lead to micro/anophthalmia, and thus performed molecular screening of its sequence in patients having micro/anophthalmia. Methods: Seventy patients having non-syndromic forms of colobomatous microphthalmia (n=25), isolated microphthalmia (n=18), or anophthalmia (n=17), and syndromic forms of micro/anophthalmia (n=10) were included in this study after negative molecular screening for OTX2, RAX, SOX2, and CHX10 mutations. Mutation screening of LHX2 was performed by direct sequencing of the coding sequences and intron/exon boundaries. Results: Two heterozygous variants of unknown significance (c.128C > G [p.Pro43Arg]; c.776C > A [p.Pro259Gln]) were identified in LHX2 among the 70 patients. These variations were not identified in a panel of 100 control patients of mixed origins. The variation c.776C > A (p.Pro259Gln) was considered as non pathogenic by in silico analysis, while the variation c.128C > G (p.Pro43Arg) considered as deleterious by in silico analysis and was inherited from the asymptomatic father. Conclusions: Mutations in LHX2 do not represent a frequent cause of micro/anophthalmia

Cryogenic R&D at the CERN Central Cryogenic Laboratory

The Central Cryogenic Laboratory operates since many years at CERN in the framework of cryogenic R&D for accelerators and experiments. The laboratory hosts several experimental posts for small cryogen ic tests, all implemented with pumping facility for GHe and vacuum, and is equipped with a He liquefier producing 6.105 l/year, which is distributed in dewars. Tests include thermomechanical qualifica tion of structural materials, cryogenic and vacuum qualification of prototypes, evaluation of thermal losses of components. Some of the most relevant results obtained at the laboratory in the last yea rs are outlined in this paper

Influence of Thermal Cycling on Cryogenic Thermometers

The stringent requirements on temperature control of the superconducting magnets for the Large Hadron Collider (LHC), impose that the cryogenic temperature sensors meet compelling demands such as long-term stability, radiation hardness, readout accuracy better than 5 mK at 1.8 K and compatibility with industrial control equipment. This paper presents the results concerning long-term stability of resistance temperature sensors submitted to cryogenic thermal cycles. For this task a simple test facility has been designed, constructed and put into operation for cycling simultaneously 115 cryogenic thermometers between 300 K and 4.2 K. A thermal cycle is set to last 71/4 hours: 3 hours for either cooling down or warming up the sensors and 1 respectively 1/4 hour at steady temperature conditions at each end of the temperature cycle. A Programmable Logic Controller (PLC) drives automatically this operation by reading 2 thermometers and actuating on 3 valves and 1 heater. The first thermal cycle was accomplished in a temperature calibration facility and all the thermometers were recalibrated again after 10, 25 and 50 cycles. Care is taken in order not to expose the sensing elements to moisture that can reputedly affect the performance of some of the sensors under investigation. The temperature sensors included Allen-Bradley and TVO carbon resistors, Cernox, thin-film germanium, thin-film and wire-wound Rh-Fe sensors

New cryogenic facilities for testing superconducting equipments for the CERN Large Hadron Collider

CERN's major project, the Large Hadron Collider (LHC), has moved to an implementation phase with machine construction to be completed by 2005. To achieve the design proton-proton centre of mass energy of 14 TeV in the given 27 km circumference LEP tunnel, the LHC will make an extensive use of high-field superconducting magnets using Nb-Ti filament operated at 1.9 K. In order to test, on the one han d, the superconducting cables of the magnets and, on the other hand, the expected performance of several of these magnets assembled in a string representing the lattice period of the machine (107 m lo ng), CERN has installed new cryogenic test facilities. The paper briefly describes these new facilities with all their associated equipments

Heat Flow Measurements on LHC Components

The refrigeration and liquefaction capacity necessary to operate at 1.9 K the 27 km long string of superconducting magnets of the LHC has been determined on the basis of heat load estimates, including static heat inleaks from ambient temperature, resistive heating and dynamic beam-induced heat loads. At all temperature levels, the static heat inleaks determine at least one third of the total heat loads in nominal operating conditions of the machine. Design validation of individual cryocomponents therefore requires a correct estimate of the heat inleaks they induce at all temperature levels, in order not to exceed the allocated heat budget. This paper illustrates the measurements of heat inleaks for several cold components of the future machine, including insulating supports, radiation shields, multi-layer insulation, instrumentation current leads. Distinct methods to determine the heat flow are chosen, depending on the expected heat loads, the temperature range spanned by the heat intercepts, and the working conditions of the component itself