癌細胞由来の新規ペリオスチンアイソフォームは頭頸部扁平上皮癌の浸潤を促進する

It recently has been reported that partial-epithelial–mesenchymal transition (p-EMT) program is associated with metastasis in head and neck squamous cell carcinoma (HNSCC). We previously have identified POSTN (which encodes periostin) as an invasion-promoting molecule in HNSCC. Interestingly, POSTN expression is frequently observed in cancer cells with higher p-EMT score by using a previous single-cell transcriptomic data of HNSCC cases. Although it is known that POSTN has 11 splicing variants, the role of them has not been determined in HNSCC. Here, we found that HNSCC cells with EMT features expressed POSTN isoforms, Iso3 (lacking exon 17 and 21) and Iso5 (lacking exon 17), whereas fibroblast expressed Iso3 and Iso4 (lacking exon 17, 18, and 21). The expression of POSTN Iso3 and Iso4 are known to be widely observed in various cell types including stromal cells. Therefore, we focused on the role of novel cancer cell-derived POSTN isoform, Iso5, in HNSCC. Single overexpression of POSTN Iso5 as well as Iso3 promoted invasion. Surprisingly, Iso5 synergistically promoted invasion together with Iso3. Notably, Iso5 as well as Iso3 upregulated p-EMT-related genes. We suggest that a novel cancer-specific POSTN isoform lacking exon 17 (Iso5) can be a useful marker for detecting cancer cells undergoing p-EMT. Moreover, a POSTN Iso5 can be a novel target for diagnosis and therapy in HNSCC

SILVERRUSH. XIII. A Catalog of 20,567 Lyα Emitters at z = 2−7 Identified in the Full-depth Data of the Subaru/HSC-SSP and CHORUS Surveys

We present 20,567 Ly α emitters (LAEs) at z = 2.2 − 7.3 that are photometrically identified by the SILVERRUSH program in a large survey area up to 25 deg ^2 with deep images of five broadband filters ( grizy ) and seven narrowband filters targeting Ly α lines at z = 2.2, 3.3, 4.9, 5.7, 6.6, 7.0, and 7.3 taken by the Hyper Suprime-Cam Subaru Strategic Program and the Cosmic HydrOgen Reionization Unveiled with Subaru survey. We select secure >5 σ sources showing narrowband color excesses via Ly α break screening, taking into account the spatial inhomogeneity of limiting magnitudes. After removing spurious sources by careful masking and visual inspection of coadded and multiepoch images obtained over the 7 yr of the surveys, we construct LAE samples consisting of 6995, 4641, 726, 6124, 2058, 18, and 5 LAEs at z = 2.2, 3.3, 4.9, 5.7, 6.6, 7.0, and 7.3, respectively, although the z = 7.3 candidates are tentative. Our LAE catalogs contain 289 spectroscopically confirmed LAEs at the expected redshifts from previous work. We demonstrate that the number counts of our LAEs are consistent with previous studies with similar LAE selection criteria. The LAE catalogs will be made public on our project webpage with detailed descriptions of the content and ancillary information about the masks and limiting magnitudes