Towards the re-verification of process tank calibrations

Re-verification is needed to ensure that the calibration (the relationship between measured level and measured volume) that is obtained during commissioning hasn’t changed over time. This can be achieved, in part, by metering in solution and correlating with marks identified a priori. Mark identification and correlation are discussed and possible error sources are outlined

Soft skills: An important asset acquired from organizing regional student group activities

Contributing to a student organization, such as the International Society for Computational Biology Student Council (ISCB-SC) and its Regional Student Group (RSG) program, takes time and energy. Both are scarce commodities, especially when you are trying to find your place in the world of computational biology as a graduate student. It comes as no surprise that organizing ISCB-SC-related activities sometimes interferes with day-to-day research and shakes up your priority list. However, we unanimously agree that the rewards, both in the short as well as the long term, make the time spent on these extracurricular activities more than worth it. In this article, we will explain what makes this so worthwhile: soft skills

Probing populations of red giants in the galactic disk with CoRoT

The detection with CoRoT of solar-like oscillations in nearly 800 red giants in the first 150-days long observational run paves the way for detailed studies of populations of galactic-disk red giants. We investigate which information on the observed population can be recovered by the distribution of the observed seismic constraints: the frequency of maximum oscillation power (nu_max) and the large frequency separation (Deltanu). We propose to use the observed distribution of nu_max and of Deltanu as a tool for investigating the properties of galactic red-giant stars through comparison with simulated distributions based on synthetic stellar populations. We can clearly identify the bulk of the red giants observed by CoRoT as red-clump stars, i.e. post-flash core-He-burning stars. The distribution of nu_max and of Deltanu gives us access to the distribution of the stellar radius and mass, and thus represent a most promising probe of the age and star formation rate of the disk, and of the mass-loss rate during the red-giant branch. CoRoT observations are supplying seismic constraints for the most populated class of He-burning stars in the galactic disk. This opens a new access gate to probing the properties of red-giant stars that, coupled with classical observations, promises to extend our knowledge of these advanced phases of stellar evolution and to add relevant constraints to models of composite stellar populations in the Galaxy.Comment: 4 pages, 6 figures, accepted in A&A Letter

Modal characteristics of a flexible tube in turbulent axial flow: a numerical approach and validation with experimental data

Flow-induced vibration is an important concern in the design of tube bundles. Due to the coupling of fluid motion and structural motion, instabilities such as flutter and divergence can arise. Next to the instabilities caused by the coupling of fluid motion and structural motion, turbulence could cause small amplitude vibrations, which in turn could give rise to long-term damage. Currently, the dynamical behavior of a tube in axial flow is studied by splitting the flow forces into inviscid and viscous components. The inviscid flow forces are determined from potential flow theory while the viscous flow forces come from empirical formulations. In this paper, a computational methodology is proposed to improve the accuracy of the predicted dynamical behaviour. In this methodology partitioned fluid-structure interaction simulations are performed to calculate the free vibration decay of a tube in axial flow. The tube is initially deformed according to an eigenmode in vacuum. Modal characteristics are then derived from the free vibration decay of the tube surrounded by the turbulent water flow. To validate this computational methodology a series of experiments is reproduced. In these experiments the frequency and damping of the fundamental mode of a solid brass cylinder were measured

Kinetic Characterization and X-ray Structure of a Mutant of Haloalkane Dehalogenase with Higher Catalytic Activity and Modified Substrate Range

Conversion of halogenated aliphatics by haloalkane dehalogenase proceeds via the formation of a covalent alkyl-enzyme intermediate which is subsequently hydrolyzed by water. In the wild type enzyme, the slowest step for both 1,2-dichloroethane and 1,2-dibromoethane conversion is a unimolecular enzyme isomerization preceding rapid halide dissociation. Phenylalanine 172 is located in a helix-loop-helix structure that covers the active site cavity of the enzyme, interacts with the Clβ of 1,2-dichloroethane during catalysis, and could be involved in stabilization of this helix-loop-helix region of the cap domain of the enzyme. To obtain more information about the role of this residue in dehalogenase function, we performed a mutational analysis of position 172 and studied the kinetics and X-ray structure of the Phe172Trp enzyme. The Phe172Trp mutant had a 10-fold higher kcat/Km for 1-chlorohexane and a 2-fold higher kcat for 1,2-dibromoethane than the wild-type enzyme. The X-ray structure of the Phe172Trp enzyme showed a local conformational change in the helix-loop-helix region that covers the active site. This could explain the elevated activity for 1-chlorohexane of the Phe172Trp enzyme, since it allows this large substrate to bind more easily in the active site cavity. Pre-steady-state kinetic analysis showed that the increase in kcat found for 1,2-dibromoethane conversion could be attributed to an increase in the rate of an enzyme isomerization step that preceeds halide release. The observed conformational difference between the helix-loop-helix structures of the wild-type enzyme and the faster mutant suggests that the isomerization required for halide release could be a conformational change that takes place in this region of the cap domain of the dehalogenase. It is proposed that Phe172 is involved in stabilization of the helix-loop-helix structure that covers the active site of the enzyme and creates a rigid hydrophobic cavity for small apolar halogenated alkanes.

Multiperiodicity in the large-amplitude rapidly-rotating β\beta Ceph ei star HD 203664

We perform a seismic study of the young massive $\beta$Cephei star HD 203664 with the goal to constrain its interior structure. Our study is based on a time series of 328 new Geneva 7-colour photometric data of the star spread over 496.8 days. The data confirm the frequency of the dominant mode of the star which we refine to $f_1=6.02885$c d$^{-1}$. The mode has a large amplitude of 37 mmag in V and is unambiguously identified as a dipole mode ($\ell=2$) from its amplitude ratios and non-adiabatic computations. Besides $f_1$, we discover two additional new frequencies in the star with amplitudes above $4\sigma$: $f_2=6.82902$c d$^{-1}$ and $f_3=4.81543$c d$^{-1}$ or one of their daily aliases. The amplitudes of these two modes are only between 3 and 4 mmag which explains why they were not detected before. Their amplitude ratios are too uncertain for mode identification. We show that the observed oscillation spectrum of HD 203664 is compatible with standard stellar models but that we have insufficient information for asteroseismic inferences. Among the large-amplitude $\beta$Cephei stars, HD 203664 stands out as the only one rotating at a significant fraction of its critical rotation velocity ($\sim 40$).Comment: 7 pages, 5 figures, accepted for publication in A&A (Astronomy & Astrophysics