A review of the relationship between eggshell colour and water vapour conductance

This review brings together the available literature that examines the effect of variations in pigmentation on the gas conductance of avian eggshells. Pheasant and Houbara bustard eggs provide some evidence that shell abnormalities can impact upon colour and conductance. By contrast, data from wild birds suggest that neither the degree of maculation or intensity of ground colour impact upon gas conductance. Those studies that purport to show variation in rates of water loss in eggs with different degrees of pigmentation may have been confounded by variation in nest humidity. This emerging field of research remains very much in its infancy

Morphological and histochemical observations of the organic components of ostrich eggshell

The organic component of the avian eggshell can be divided into 3 portions, the shell membranes, the matrix and the cuticle. These have been well characterised in the chicken but little has been published with regard to the ostrich (Struthio camelus). A number of recent studies have indicated that the cause of intra-shell embryonic deaths in the ostrich is similar to intra-shell embryonic deaths that occur in the chicken. These deaths in the chicken are associated with the loss of or damage to the waxy cuticle and other organic components of the eggshell, which is reported to be absent in the ostrich eggshell. In this study, preliminary morphological and histochemical analyses, at the level of the light and electron microscope, have characterised the various organic components of the ostrich eggshell. The results of the histochemical and electron microscopical analyses suggest that there mayonly be 1 shell membrane in this species, which could play a major role in the limitation of bacterial penetration to the embryonic chamber. The shell membrane has a distinct elemental profile as determined by EDSanalysis. The matrix is shown to decrease in mesh size from the mammillary layer to the vertical crystal layer. The closer packing of the mesh may indicate the presence of a morphologically discernible termination signal to calcification or the remnants of an evolutionary calcified cuticle. The matrix of the pores may also forma defensive barrier against bacterial invasion, which could be damaged as a result of dipping the eggs before incubation