Effectiveness of vegetative filter strips

The use of close-growing plants, such as in grass sod, is a long-established technique for controlling soil erosion. This approach serves two purposes: (1) the fine root system of sod holds soil in place, thereby reducing its susceptibility to erosion, and (2) the plants slow the velocity of water flow, which reduces the sediment-carrying capacity of the wate

Riparian Management for Water Quality, the Bear Creek Example: Getting the Message Out

While a considerable body of evidence confirms that exiting vegetated streamside zones can be effective sinks for nonpoint source pollution (Castelle et al. 1994, Osborne and Kovacic 1993, Lowrance 1992, Cooper et al. 1987, Jacobs and Gilliam 1985, Lowrance et al. 1985, 1984, Peterjohn and Correll 1984), little information is available for restored or constructed streamside buffer systems. To demonstrate the benefits of properly functioning riparian zones in the heavily row-cropped midwestern U.S., the Agroecology Issue Team of the Leopold Center for Sustainable Agriculture and the Iowa State Agroforestry Research Team (IStART) are conducting research on the design and establishment of integrated riparian management systems. The purpose of these systems is to restore the essential ecological functions that these riparian areas once provided. Specific objectives of such buffers are to intercept eroding soil and agricultural chemicals from adjacent crop fields, slow flood waters, stabilize streambanks, provide wildlife habitat, and improve the biological integrity of aquatic ecosystems

Regulation of Transcriptional Activity during the First and Second Cell Cycles in the Preimplantation Mouse Embryo

AbstractTranscription of endogenous genes in preimplantation 1- and 2-cell mouse embryos was determined by monitoring the incorporation of BrUTP by plasma membrane-permeabilized embryos. Incorporation is observed starting by mid-S phase in the 1-cell embryo and increases progressively; the amount of incorporation by the 1-cell embryo in G2 is about 20% that of the 2-cell embryo in G2. Incorporation by the male pronucleus is always about four to five times greater than that of the female pronucleus. Nevertheless, the amount of incorporation by the female pronucleus present in parthogenetically activated eggs is similar to the total amount of incorporation in inseminated eggs, i.e., the transcriptional capacity of the female pronucleus is not inherently less than that of the male pronucleus. Inhibiting the first round of DNA replication does not prevent the initiation of transcription in the 1-cell embryo, but does inhibit the extent of BrUTP incorporation by 35%. The transcriptional machinery of the 1-cell embryo appears to be rate-limiting, since the total amount of BrUTP incorporation by parthenogenetically activated and dispermic eggs is similar to that in monospermic eggs; trispermic eggs incorporate BrUTP to only about 60% the level of monospermic eggs. A transcriptionally repressive state may start to develop in the 2-cell embryo, since inhibiting the second round of DNA replication results in an 50% increase in BrUTP incorporation. Trapoxin treatment, which induces histone hyperacetylation, enhances incorporation by 2-cell embryos 1.8-fold and suggests that histone hyperacetylation can relieve this repression

Role of MAP kinase and myosin light chain kinase in chromosome-induced development of mouse egg polarity

AbstractDuring maturation, the mouse oocyte is transformed into a highly polarized egg, characterized by an actin cap and cortical granule-free domain (CGFD) overlying the meiotic spindle that is in close proximity to the cortex. The presence of spindle/chromosomes or microinjected sperm chromatin in the cortical region initiates this cortical reorganization, but the pathway is unknown. We report that cortical reorganization induced by microinjected sperm chromatin is blocked by inhibitors of microfilament assembly or disassembly. Active mitogen-activated protein kinase (MAPK), which becomes enriched in the region of sperm chromatin, is required for cortical reorganization, because microinjected sperm chromatin fails to induce cortical reorganization in Mos−/− eggs, which lack MAPK activity. Last, myosin light chain kinase (MLCK), which can be directly phosphorylated and activated by MAPK, appears involved, because the MLCK inhibitors ML-7 and Peptide 18 prevent sperm chromatin-induced cortical reorganization. These results provide new insights into how cortical reorganization occurs independently of extracellular signals to generate egg polarity

Transcript profiling during preimplantation mouse development

AbstractStudies using low-resolution methods to assess gene expression during preimplantation mouse development indicate that changes in gene expression either precede or occur concomitantly with the major morphological transitions, that is, conversion of the oocyte to totipotent 2-cell blastomeres, compaction, and blastocyst formation. Using microarrays, we characterized global changes in gene expression and used Expression Analysis Systematic Explorer (EASE) to identify biological and molecular processes that accompany and likely underlie these transitions. The analysis confirmed previously described processes or events, but more important, EASE revealed new insights. Response to DNA damage and DNA repair genes are overrepresented in the oocyte compared to 1-cell through blastocyst stages and may reflect the oocyte's response to selective pressures to insure genomic integrity; fertilization results in changes in the transcript profile in the 1-cell embryo that are far greater than previously recognized; and genome activation during 2-cell stage may not be as global and promiscuous as previously proposed, but rather far more selective, with genes involved in transcription and RNA processing being preferentially expressed. These results validate this hypothesis-generating approach by identifying genes involved in critical biological processes that can be the subject of a more traditional hypothesis-driven approach

High stage events and stream bank erosion on small grazed pasture stream reaches in the rathbun lake watershed, southern IOWA, USA

Stream bank erosion in agricultural landscapes is a major pathway for non-point source sediment and phosphorus loading of receiving waters. Previous studies have shown direct and indirect effects of land use on stream bank erosion, and identified high erosion rates within riparian pastures. One potential impact of agricultural land-use on stream bank erosion is the alteration of stream stage characteristics, including an increase in frequency of high-stage events over short periods of time (forming flash hydrographs). The objective of this study was to assess the relationship between the number of high stream stages and corresponding stream bank soil erosion. The study was conducted in six grazed pasture stream reaches within the Rathbun Lake Watershed, a reservoir on the Chariton River located within the Southern Iowa Drift Plain. The erosion pin method was utilized to measure the change in stream bank erosion in response to differences in the number of high stream-stage events, which were monitored by pressure transducers. The measured seasonal bank erosion rates were correlated with the different stream stages data to assess their impact on stream bank erosion. Based on the different model assumptions, there were generally strong linear relationships between high stage and bank erosion. Approximately 75% of the variability in stream bank erosion rates was directly linked to the number of high stages/erosive stream flow depths. Conservation practices that reduce these erosion rates will be those that increase soil-water infiltration, reduce the frequency of high stream flow events and increase bank stability through perennial vegetation cover or reducing disturbance within the riparian zone