Encounters between Dynamic Cortical Microtubules Promote Ordering of the Cortical Array through Angle-Dependent Modifications of Microtubule Behavior

Ordered cortical microtubule arrays are essential for normal plant morphogenesis, but how these arrays form is unclear. The dynamics of individual cortical microtubules are stochastic and cannot fully account for the observed order; however, using tobacco (Nicotiana tabacum) cells expressing either the MBD-DsRed (microtubule binding domain of the mammalian MAP4 fused to the Discosoma sp red fluorescent protein) or YFP-TUA6 (yellow fluorescent protein fused to the Arabidopsis alpha-tubulin 6 isoform) microtubule markers, we identified intermicrotubule interactions that modify their stochastic behaviors. The intermicrotubule interactions occur when the growing plus-ends of cortical microtubules encounter previously existing cortical microtubules. Importantly, the outcome of such encounters depends on the angle at which they occur: steep-angle collisions are characterized by approximately sevenfold shorter microtubule contact times compared with shallow-angle encounters, and steep-angle collisions are twice as likely to result in microtubule depolymerization. Hence, steep-angle collisions promote microtubule destabilization, whereas shallow-angle encounters promote both microtubule stabilization and coalignment. Monte Carlo modeling of the behavior of simulated microtubules, according to the observed behavior of transverse and longitudinally oriented cortical microtubules in cells, reveals that these simple rules for intermicrotubule interactions are necessary and sufficient to facilitate the self-organization of dynamic microtubules into a parallel configuration

The Localization Industry: A Profile of DNA Media

Since the mid-1990s the e-commerce industry experienced dramatic growth that was only the start of a business revolution. With the rapid expansion of Internet related infrastructure equipment and services that allowed low-cost global communications, the beginnings of a truly global economy began to take shape. Riding on the coat tails of this wave was software and content localization services that were a necessary component in selling products and services to different countries and across many cultures. The challenges of operating in a diverse, multicultural market are great, filled with cultural subtleties that can be a minefield for the uninformed. DNA Media, based in Vancouver, Canada, is a software localization company specializing in language, software application and content (Web-based technologies, application design, CD-ROM, DVD and multi-media versioning). The company enjoyed strong growth in its services in the last two years and, by the year 2000 it was in a position to expand rapidly. This case provides insight into how managers of a small but growing information technology company managed its growth, established its market in the software localization industry, and planned for the next phase of expansion

Development of heat flux sensors for turbine airfoils

The objectives of this program are to develop heat flux sensors suitable for installation in hot section airfoils of advanced aircraft turbine engines and to experimentally verify the operation of these heat flux sensors in a cylinder in a cross flow experiment. Embedded thermocouple and Gardon gauge sensors were developed and fabricated into both blades and vanes. These were then calibrated using a quartz lamp bank heat source and finally subjected to thermal cycle and thermal soak testing. These sensors were also fabricated into cylindrical test pieces and tested in a burner exhaust to verify heat flux measurements produced by these sensors. The results of the cylinder in cross flow tests are given

Stereociliary Myosin-1c Receptors Are Sensitive to Calcium Chelation and Absent from Cadherin 23 Mutant Mice

The identities of some of the constituents of the hair-cell transduction apparatus have been elucidated only recently. The molecular motor myosin-1c (Myo1c) functions in adaptation of the hair-cell response to sustained mechanical stimuli and is therefore an integral part of the transduction complex. Recent data indicate that Myo1c interacts in vitro with two other molecules proposed to be important for transduction: cadherin 23 (Cdh23), a candidate for the stereociliary tip link, and phosphatidylinositol 4,5-bisphosphate (PIP2), which is abundant in the membranes of hair-cell stereocilia. It is not known, however, whether these interactions occur in hair cells. Using an in situ binding assay on saccular hair cells, we demonstrated previously that Myo1c interacts with molecules at stereociliary tips, the site of transduction, through sequences contained within its calmodulin (CaM)-binding neck domain, which can bind up to four CaM molecules. In the current study, we identify the second CaM-binding IQ domain as a region of Myo1c that mediates CaM-sensitive binding to stereociliary tips and to PIP2 immobilized on a solid support. Binding of Myo1c to stereociliary tips of cochlear and vestibular hair cells is disrupted by treatments that break tip links. In addition, Myo1c does not bind to stereocilia from mice whose hair cells lack Cdh23 protein despite the presence of PIP2 in the stereociliary membranes. Collectively, our data suggest that Myo1c and Cdh23 interact at the tips of hair-cell stereocilia and that this interaction is modulated by CaM

Strategies for Managing Water Resources in Saltwater Intrusion Environments

2008 S.C. Water Resources Conference - Addressing Water Challenges Facing the State and Regio

Automating the Generation of High School Geometry Proofs using Prolog in an Educational Context

When working on intelligent tutor systems designed for mathematics education and its specificities, an interesting objective is to provide relevant help to the students by anticipating their next steps. This can only be done by knowing, beforehand, the possible ways to solve a problem. Hence the need for an automated theorem prover that provide proofs as they would be written by a student. To achieve this objective, logic programming is a natural tool due to the similarity of its reasoning with a mathematical proof by inference. In this paper, we present the core ideas we used to implement such a prover, from its encoding in Prolog to the generation of the complete set of proofs. However, when dealing with educational aspects, there are many challenges to overcome. We also present the main issues we encountered, as well as the chosen solutions.Comment: In Proceedings ThEdu'19, arXiv:2002.1189

Le FORUM, Vol. 38 No. 1

https://digitalcommons.library.umaine.edu/francoamericain_forum/1040/thumbnail.jp

Addressing decontaminated respirators: Some methods appear to damage mask integrity and protective function

Decontamination of N95 respirators is being used by clinicians in the face of a global shortage of these devices. Some treatments for decontamination, such as some vaporized hydrogen peroxide methods or ultraviolet methods, had no impact on respiratory performance, while other treatments resulted in substantial damage to masks