Le Traitement Chirurgical Des Poches De Rétraction Tympaniques

Les poches de rétractions représentent un aspect particulier de l\'otite moyenne chronique affectant la morphologie tympanique et pouvant avoir des répercussions fonctionnelles auditives. L\'évolution naturelle de l\'otite atelectasique vers la maladie cholestéatomateuse complique d\'avantage la prise en charge des poches de rétraction. Les auteurs rapportent une étude rétrospective à propos de 137 cas de poches de rétraction colligées sur une période de 10 ans de 1992 à 2001. Le traitement chirurgical des poches de rétraction pose la problématique du bon choix de la technique opératoire et de l\'indication de la chirurgie de renforcement.Retraction pockets represent a particular aspect of chronic otitis whish affect tympanic membran morphology and can influence hearing potentialities. The evolution course of atelectasic disease leeding to localized retraction may be the principal cause for cholesteatoma witch complicates the management of the retraction pockets. The authors report a retrospective study about 137cases of retraction pockets collaged from 1992 to 2001. The problems of the surgical treatement of the retraction pockets include the best choice of the operative technique for reconstructive tynpanoplastie. Journal Tunisien d\'ORL et de chirurgie cervico-faciale Vol. 16 2006: pp. 18-2

Mertensene, a halogenated monoterpene, induces G2/M cell cycle arrest and caspase dependent apoptosis of human colon adenocarcinoma HT29 cell line through the modulation of ERK-1/-2, AKT and NF-κB signaling

Conventional treatment of advanced colorectal cancer is associated with tumor resistance and toxicity towards normal tissues. Therefore, development of effective anticancer therapeutic alternatives is still urgently required. Nowadays, marine secondary metabolites have been extensively investigated due to the fact that they frequently exhibit anti-tumor properties. However, little attention has been given to terpenoids isolated from seaweeds. In this study, we isolated the halogenated monoterpene mertensene from the red alga Pterocladiella capillacea (S.G. Gmelin) Santelices and Hommersand and we highlight its inhibitory effect on the viability of two human colorectal adenocarcinoma cell lines HT29 and LS174. Interestingly, exposure of HT29 cells to different concentrations of mertensene correlated with the activation of MAPK ERK-1/-2, Akt and NF-κB pathways. Moreover, mertensene-induced G2/M cell cycle arrest was associated with a decrease in the phosphorylated forms of the anti-tumor transcription factor p53, retinoblastoma protein (Rb), cdc2 and chkp2. Indeed, a reduction of the cellular level of cyclin-dependent kinases CDK2 and CDK4 was observed in mertensene-treated cells. We also demonstrated that mertensene triggers a caspase-dependent apoptosis in HT29 cancer cells characterized by the activation of caspase-3 and the cleavage of poly (ADP-ribose) polymerase (PARP). Besides, the level of death receptor-associated protein TRADD increased significantly in a concentration-dependent manner. Taken together, these results demonstrate the potential of mertensene as a drug candidate for the treatment of colon cancer. © 2017 by the authors. Licensee MDPI