Utjecaj stresa pri prijevozu na kortizol, hormone štitnjače i neke biokemijske pokazatelje u serumu iranske kašmirske (Raini) koze.

In this study the effect of transportation stress on serum concentrations of cortisol, thyroid hormones, β-hydroxybutyrate, nonesterified fatty acids, glucose and plasma total protein in Iranian cashmere (Raini) goats were evaluated. On the first day, blood samples from 10 Raini goats (5 males and 5 non-lactating nonpregnant females) were collected at 8 A.M. (T1), and after 3h food and water deprivation (T2). On the second day, samples were collected at 8 A.M. (T3), and after 3h transport (T4). Final blood sample was taken 24 h after transport ended (T5). Food and water deprivation caused a significant decrement in serum cortisol, and a significant increase between T2 and T3 was evident. Transportation caused a significant increase in serum cortisol, and the increase continued until T5. Serum T3 showed a marginally significant decrease due to the food and water deprivation. Serum T4 and NEFA significantly increased between T2 and T3. No significant change in serum concentrations of glucose, fT3, fT4, T3/T4 ratio and BHBA in different samplings was observed. The current study showed that short road transport had a significant effect on some stress biomarkers in cashmere goats, however, it takes a longer time before hormonal control of metabolism can be affected.U iranskih kašmirskih (Raini) koza istražen je učinak stresa pri prijevozu na koncentraciju kortizola u serumu, hormone štitnjače, β-hidroksibutirat, neesterificirane masne kiseline, glukozu i ukupne bjelančevine plazme. Prvi dan, u 8,00 sati (T1), uzeti su uzorci krvi od 10 Raini koza (5 mužjaka i 5 ženki koje nisu bile u laktaciji ni gravidne). Uzorci krvi ponovno su uzeti tri sata (T2) nakon što je kozama bila uskraćena hrana i voda. Drugog dana uzorci su prikupljeni u 8,00 sati (T3), te nakon 3 sata prijevoza (T4). Konačni uzorak krvi uzet je 24 sata nakon što je prijevoz završio (T5). Kortizol u serumu nakon uskraćivanja hrane i vode bio je značajno snižen, a između T2 i T3 značajno povišen. Prijevoz je uzrokovao značajan porast kortizola u serumu što je nastavljeno do T5. T3 u serumu je pokazao granično značajan pad zbog uskraćivanja hrane i vode. T4 i NEFA u serumu bili su značajno povećani između T2 i T3. U različitim uzorcima nisu utvrđene značajne promjene u serumskim koncentracijama glukoze, fT3, fT4, omjeru T3/T4 i BHBA. Istraživanje je pokazalo da kratki cestovni prijevoz ima značajan učinak na neke biomarkere stresa u kašmirskih koza, međutim, za utjecaj na hormonsku kontrolu metabolizma potrebno je duže vremensko razdoblje