Touching maths in our environment

RESUMEN: Con este Trabajo de Fin de Grado (TFG) se busca informar a los docentes y alumnos sobre los Paseos Matemáticos, además de proponer su uso como herramienta complementaria para trabajar los contenidos matemáticos del vigente currículum de Cantabria. Se desarrollará un ejemplo en el Centro Concertado de Calasanz de Santander para alumnos de primero de Educación Primaria y se expondrán los resultados de su puesta en práctica. Se pretende también presentar y analizar la herramienta MathCityMaps, que permite crear y compartir paseos matemáticos de forma sencilla.ABSTRACT: The objective of this Bachelor’s Dissertation is to introduce teachers and scholars to the concept of the Mathematical Walk. In addition, it aims to be an interesting tool for working on the mathematical content prescribed in the current curriculum of Cantabria. An example is implemented in the Calasanz Concerted Centre in Santander for pupils in the first year of primary school and the results obtained are shown. It also presents MathCityMaps, an application that allows to easily create and share Mathematical Walks.Grado en Magisterio en Educación Primari

6-O-Nucleotidyltransferase: an aminoglycoside-modifying enzyme specific for streptomycin/streptidine

Aminoglycosides are especially useful for the treatment of hospital-acquired infections. The main problem for the application of these antibiotics is the presence of bacterial resistance enzymes, in particular, nucleotidyltransferases (ANTs). These enzymes catalyze the transfer of an adenylyl group from the MgATP complex to different positions of the antibiotic. To understand the mechanisms that lead to antibiotic inactivation, we have performed a comprehensive experimental analysis of one of those enzymes. The 6-O- nucleotidyltransferase enzyme (ANT(6)) from Bacillus subtilis was cloned, overexpressed and purified in E. coli. The kinetic parameters revealed a narrow specificity of the ANT(6) for MgATP/streptomycin as substrates. The binding epitope of the streptomycin recognized by the ANT(6) is the streptidine moiety. Therefore, the use of streptidine as a “decoy acceptor” allows the recovery of the antibiotic activity of streptomycin E. coli cells that are overexpressing the ANT(6).This investigation was supported by a research grant of the Span-ish research “Dirección General de Investigación" CTQ2013-45538-PPeer reviewe

Preparation and Characterization of Aminoglycoside-Loaded Chitosan/Tripolyphosphate/Alginate Microspheres against E. coli

Although aminoglycosides are one of the common classes of antibiotics that have been widely used for treating infections caused by pathogenic bacteria, the evolution of bacterial resistance mechanisms and their inherent toxicity have diminished their applicability. Biocompatible carrier systems can help sustain and control the delivery of antibacterial compounds while reducing the chances of antibacterial resistance or accumulation in unwanted tissues. In this study, novel chitosan gel beads were synthesized by a double ionic co-crosslinking mechanism. Tripolyphosphate and alginate, a polysaccharide obtained from marine brown algae, were employed as ionic cross-linkers to prepare the chitosan-based networks of gel beads. The in vitro release of streptomycin and kanamycin A was bimodal; an initial burst release was observed followed by a diffusion mediated sustained release, based on a Fickian diffusion mechanism. Finally, in terms of antibacterial properties, the particles resulted in growth inhibition of Gram-negative (E. coli) bacteria

Design of a biocatalytic cascade for the enzymatic sulfation of unsulfated chondroitin with in situ generation of PAPS

Sulfation of molecules in living organisms is a process that plays a key role in their functionality. In mammals, the sulfation of polysaccharides (glycosaminoglycans) that form the proteoglycans present in the extracellular matrix is particularly important. These polysaccharides, through their degree and sulfation pattern, are involved in a variety of biological events as signal modulators in communication processes between the cell and its environment. Because of this great biological importance, there is a growing interest in the development of efficient and sustainable sulfation processes, such as those based on the use of sulfotransferase enzymes. These enzymes have the disadvantage of being 3′-phosphoadenosine 5′-phosphosulfate (PAPS) dependent, which is expensive and difficult to obtain. In the present study, a modular multienzyme system was developed to allow the in situ synthesis of PAPS and its coupling to a chondroitin sulfation system. For this purpose, the bifunctional enzyme PAPS synthase 1 (PAPSS1) from Homo sapiens, which contains the ATP sulfurylase and APS kinase activities in a single protein, and the enzyme chondroitin 4-O-sulfotransferase (C4ST-1) from Rattus norvegicus were overexpressed in E. coli. The product formed after coupling of the PAPS generation system and the chondroitin sulfation module was analyzed by NMR

Chemoenzymatic preparation of Chondroitin sulfates with a defined sulfation pattern

Comunicación Oral impartida en el XVII European Congress on BiotechnologyN

Image et concception du personnage féminin présent dans les oeuvres : histoire du chevalier Des Grieux et de Manon Lescaut de l'abbbé Prévost et Les liaisons dangereuses de Choderlos de Laclos

Premi UAB de l'Observatori per a la Igualtat als millors Treballs de Fi de Grau amb perspectiva de gènere. 4a Edició, curs 2018-2019Siècle de splendeur littéraire et culturelle, le XVIIIe siècle est l'un des plus riches culturellement de l'histoire française. En effet, les deux ouvrages étudiés font partie de ce contexte, montrant deux femmes très représentatives de leur siècle. Si les protagonistes sont effectivement deux femmes, les deux auteurs sont des hommes, ce qui nous permet de voir le point de vue d'une société clairement marquée par les différences de genre. Libertines, intelligentes, séduisantes ou machiavéliques sont quelques-uns des adjectifs qui les caractérisent. Deux femmes prisonnières de leur statut et de leur culture androcentrique.Segle d'esplendor literària i cultural, el segle XVIII és un dels més rics, culturalment, de la història francesa. En efecte, les dues obres estudiades formen part d'aquest context, mostrant dues dones molt representatives del seu segle. Si bé els protagonistes són efectivament dues dones, els dos autors són homes, la qual cosa ens permet veure el punt de vista d'una societat clarament marcada per les diferències de gènere. Llibertines, intel·ligents, seductores o maquiavèl·liques són alguns dels adjectius que les caracteritzen. Dues dones presoneres de la seva condició i de la cultura androcèntric

Interdisciplinary perspectives on privacy awareness in lifelogging technology development

Population aging resulting from demographic changes requires some challenging decisions and necessary steps to be taken by different stakeholders to manage current and future demand for assistance and support. The consequences of population aging can be mitigated to some extent by assisting technologies that can support the autonomous living of older individuals and persons in need of care in their private environments as long as possible. A variety of technical solutions are already available on the market, but privacy protection is a serious, often neglected, issue when using such (assisting) technology. Thus, privacy needs to be thoroughly taken under consideration in this context. In a three-year project PAAL (‘Privacy-Aware and Acceptable Lifelogging Services for Older and Frail People’), researchers from different disciplines, such as law, rehabilitation, human-computer interaction, and computer science, investigated the phenomenon of privacy when using assistive lifelogging technologies. In concrete terms, the concept of Privacy by Design was realized using two exemplary lifelogging applications in private and professional environments. A user-centered empirical approach was applied to the lifelogging technologies, investigating the perceptions and attitudes of (older) users with different health-related and biographical profiles. The knowledge gained through the interdisciplinary collaboration can improve the implementation and optimization of assistive applications. In this paper, partners of the PAAL project present insights gained from their cross-national, interdisciplinary work regarding privacy-aware and acceptable lifelogging technologies.Open Access funding enabled and organized by Projekt DEAL. This work is part of the PAAL-project (“Privacy-Aware and Acceptable Lifelogging services for older and frail people”). The support of the Joint Programme Initiative “More Years, Better Lives” (award number: PAAL_JTC2017), the German Federal Ministry of Education and Research (grant no: 16SV7955), the Swedish Research Council for Health, Working Life, and Welfare (grant no: 2017–02302), the Spanish Agencia Estatal de Investigacion (PCIN-2017-114), the Italian Ministero dell’Istruzione dell’Universitá e della Ricerca, (CUP: I36G17000380001), and the Canadian Institutes of Health Research is gratefully acknowledged

Suicidal behaviour and cognition: A systematic review with special focus on prefrontal deficits

BACKGROUND: Suicide is a major health concern worldwide, thus, identifying risk factors would enable a more comprehensive understanding and prevention of this behaviour. Neuropsychological alterations could lead to difficulties in interpreting and managing life events resulting in a higher risk of suicide. METHOD: A systematic literature search from 2000 to 2020 was performed in Medline (Pubmed), Web of Science, SciELO Citation Index, PsycInfo, PsycArticles and Cochrane Library databases regarding studies comparing cognition of attempters versus non-attempters that share same psychiatric diagnosis. RESULTS: 1.885 patients diagnosed with an Affective Disorder (n=1512) and Schizophrenia/ Schizoaffective Disorder (n=373) were included. In general comparison, attention was found to be clearly dysfunctional. Regarding diagnosis, patients with Schizophrenia and previous history of suicidal behaviour showed a poorer performance in executive function. Patients with current symptoms of an Affective Disorder and a previous history of suicidal attempt had poorer performance in attention and executive function. Similarly, euthymic affective patients with history of suicidal behaviour had worse decision-making, attention and executive function performance compared to euthymic non-attempters. LIMITATIONS: The number of papers included in this review is limited to the few studies using non-attempter clinically-matched control group and therefore results regarding diagnosis, symptomatology and time of the attempt are modest and contradictory. CONCLUSIONS: Patients who have attempted suicide have a poorer neuropsychological functioning than non-attempters with a similar psychiatric disorder in attention and executive function. These alterations increase vulnerability for suicide.This work was supported by Carlos III Health Research Institute[grant numbers PI14/02029, PI15/00793, PI15/00789, PI16/01164,PI17/01433 and PI18/01055 (co-financed by the European RegionalDevelopment Fund(FEDER/ERDF)/European Social Fund‘Investing inyour future’ and the Government of the Principality of Asturias PCTI-2018–2022 IDI/2018/235)], Foundation for Health Innovation andResearch (BIOEF); Bioaraba Research Institute; Networking Center forBiomedical Research in Mental Health (CIBERSAM), the BasqueGovernment [grant numbers 2015111024, 2017111104] and theUniversity of the Basque Country [grant number 321212ELBY]. Thepsychiatric research department in Araba University Hospital is sup-portedbytheStanleyResearchFoundation[grantnumber03-RC-003

The Mediating Role of Impulsivity in the Relationship Between Suicidal Behavior and Early Traumatic Experiences in Depressed Subjects

Background: Depressed patients with early traumatic experiences may represent a clinically and biologically distinct subtype, with worse clinical outcomes and greater risk of suicide. Since early traumatic experiences alter development of systems that regulate the stress response, increasing sensitivity to stress and mood disorders later in life, certain personality features may influence coping strategies, putting individuals with depression and a history of early traumatic experiences at greater risk of suicidal behavior.Objective: To determine whether impulsivity mediates the relationship between early traumatic experiences and suicidal behavior in patients with major depressive disorder (MDD).Methods: The total sample consists of 190 patients [mean age (SD) = 53.71 (10.37); females: 66.3%], with current MDD (DSM-5 criteria). The Childhood Trauma Questionnaire-Short Form (CTQ-SF), the List of Threatening Experiences (LTE), and the Barratt Impulsiveness Scale-11 (BIS-11) were used to assess childhood and adulthood adverse life events and impulsivity, respectively. We developed mediation models by bootstrap sampling methods.Results: Eighty-one (42.6%) patients had a history of previous suicide attempts (SA). CTQ-SF-Total and BIS-11-Total scores were significantly higher in MDD patients with previous SA. Correlation analyses revealed significant correlations between the CTQ-SF-Total and BIS-11-Total, CTQ-SF-Total and HDRS-Total, and BIS-11-Total and HDRS-Total scores. Regression models found that CTQ-SF-Total, BIS-11-Total, and HDRS-Total scores were associated with SA. Mediation analyses further revealed the association between CTQ-SF-Total and SA was mediated by the indirect effect of the BIS-11-Total score (b = 0.007, 95% CI = 0.001, 0.015), after statistically controlling for sex, the HDRS-Total, and the LTE-Total.Discussion: Data suggest that impulsivity could mediate the influence of childhood trauma on suicidal behavior. This will help understand the role of risk factors in suicidal behavior and aid in the development of prevention interventions focused on modifiable mediators when risk factors are non-modifiable.2020-2

Cribado de la depresión mayor en la infancia y adolescencia (Parte 2)

El síndrome depresivo que aparece durante la infancia o la adolescencia tiende a seguir un curso crónico, con alta probabilidad de permanecer en la edad adulta. Su sintomatología repercute en la vida diaria del menor y, en el caso de la depresión mayor, supone un riesgo para la aparición de conducta suicida. Dada la variabilidad de sus manifestaciones clínicas y las dificultades en el diagnóstico, se plantea la posible utilidad del uso de cuestionarios de cribado en la población infantil y adolescente, a partir de un análisis del rendimiento de estos y de la eficacia de las medidas terapéuticas actuales. El presente trabajo se ha dividido en dos partes, y al final de este se recopilan las recomendaciones de distintos grupos de trabajo y tras el análisis de la evidencia, las recomendaciones que el grupo PrevInfad ha consensuado para la consulta de los pediatras en Atención Primaria