Simulations of Time-Resolved X-Ray Diffraction in Laue Geometry

A method of computer simulation of Time-Resolved X-ray Diffraction (TRXD) in asymmetric Laue (transmission) geometry with an arbitrary propagating strain perpendicular to the crystal surface is presented. We present two case studies for possible strain generation by short-pulse laser irradiation: (i) a thermoelastic-like analytic model; (ii) a numerical model including effects of electron-hole diffusion, Auger recombination, deformation potential and thermal diffusion. A comparison with recent experimental results is also presented.Comment: 9 pages, 11 figure

Marine polysaccharide multilayers: PH responsive systems for the surface modification of tissue engineering scaffolds

[Excerpt] The success of some polymeric scaffolds for Tissue Engineering is hindered by its surface chemistry, which in many cases leads to a significant foreign body response. To overcome this, the present project intend to explore a strategy of surface modification through electrostatic self-assembly, first reported in the 1990s, by the construction of multilayered systems by assembling a polycation and a polyanion in an alternate fashion. [...]info:eu-repo/semantics/publishedVersio

Extraction and characterization of collagen from elasmobranch byproducts for potential biomaterial use

With the worldwide increase of fisheries, fish wastes have had a similar increase, alternatively they can be seen as a source of novel substances for the improvement of societyâ s wellbeing. Elasmobranchs are a subclass fished in high amounts, with some species being mainly bycatch. They possess an endoskeleton composed mainly by cartilage, from which chondroitin sulfate is currently obtained. Their use as a viable source for extraction of type II collagen has been hypothesized with the envisaging of a biomedical application, namely in biomaterials production. In the present work, raw cartilage from shark (Prionace glauca) and ray (Zeachara chilensis and Bathyraja brachyurops) was obtained from a fish processing company and submitted to acidic and enzymatic extractions, to produce acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC). From all the extractions, P. glauca PSC had the highest yield (3.5%), followed by ray ASC (0.92%), ray PSC (0.50%), and P. glauca ASC (0.15%). All the extracts showed similar properties, with the SDS-PAGE profiles being compatible with the presence of both type I and type II collagens. Moreover, the collagen extracts exhibited the competence to maintain their conformation at human basal temperature, presenting a denaturation temperature higher than 3 C. Hydrogels were produced using P. glauca PSC combined with shark chondroitin sulfate, with the objective of mimicking the human cartilage extracellular matrix. These hydrogels were cohesive and structurally-stable at 37 C, with rheological measurements exhibiting a conformation of an elastic solid when submitted to shear strain with a frequency up to 4 Hz. This work revealed a sustainable strategy for the valorization of fisheriesâ by-products, within the concept of a circular economy, consisting of the use of P. glauca, Z. chilensis, and B. brachyurops cartilage for the extraction of collagen, which would be further employed in the development of hydrogels as a proof of concept of its biotechnological potential, ultimately envisaging its use in marine biomaterials to regenerate damaged cartilaginous tissues.The authors acknowledge the fish-processing industry Nigel (Peniche, Portugal) for the kind offer of shark and ray by-products, Marco Lemos (MARE-Leiria, IPLeiria, Portugal) for valuable discussions, and Filipe Costa and Sofia Duarte (CBMA, University of Minho, Portugal) for the DNA barcoding analysis for identification/confirmation of elasmobranchii species

Taxa de fertilização e vingamento de frutos de Bertholletia excelsa.

Conhecer com mais detalhes a formação e desenvolvimento dos frutos da castanheira se faz necessário para subsidiar estudos futuros que busquem maximizar a produtividade de Bertholletia excelsa em cultivos ou mesmo em áreas de extrativismo. Seu fruto tem um alto valor nutricional, sendo rico em proteínas, fibras, vitaminas, lipídios, minerais e selênio, e é um produto que pode ser consumido tanto in natura como industrializado. É um dos principais produtos florestais não-madeireiros que gera renda para as famílias residentes em florestas, na Amazônia, sendo amplamente reconhecida como uma espécie modelo para a conservação florestal. O presente trabalho tem por finalidade avaliar aspectos visuais do desenvolvimento de frutos da castanheira a fim de auxiliar em estudo futuro para determinação da curva de crescimento dos frutos e sementes de castanha-do-brasil. O número médio de flores por inflorescência foi de 139,6. Nas inflorescências manipuladas apenas 2,7% das flores contadas geraram frutos, enquanto que em inflorescências não manipuladas esse valor seria da ordem de 10%. Observou-se 75% de aborto nas inflorescências manipuladas e nas não manipuladas nenhum aborto foi observado após 70 dias

Nanostructured natural-based polyelectrolyte multilayers to agglomerate chitosan particles into scaffolds for tissue engineering

The Layer-by-Layer (LbL) deposition technique is a self-assembly process that allows the coating of material's surface with nanostructured layers of polyelectrolytes, allowing to control several surface properties. This technique presents some advantages when compared with other thin film assembly techniques like having the possibility to coat surfaces with complex geometries in mild conditions or to incorporate active compounds. Tissue engineering involves typically the use of porous biodegradable scaffolds for the temporary support of cells. Such structures can be produced by agglomeration of micro-spheres that needs to be fixed into a three dimensional structure. In this work we suggest the use of LbL to promote such mechanical fixation in free-formed micro-spheres assemblies and simultaneously to control the properties of its surface. For the proof of concept the biological performance of chitosan/alginate multilayers is first investigated in two-dimensional models in which the attachment and proliferation of L929 and ATDC5 cells are studied in function of the number of layers and the nature of the final layer. Scaffolds prepared by agglomeration of chitosan particles using the same multilayered system were processed and characterized; it was found that they could support the attachment and proliferation of ATDC5 cells. This study suggests that LbL can be used as a versatile methodology to prepare scaffolds by particle agglomeration that could be suitable for tissue engineering applications.Fundo Europeu de Desenvolvimento Regional (FEDER) através do Programa Operacional de Cooperação Transfronteiriça Espanha Portugal 2007-2013 (POCTEP)Fundação para a Ciência e a Tecnologia (FCT

Seasonal Botanical Composition and Available Forage of Natural Grasslands in the Southeastern Range Region of Rio Grande Do Sul, Brazil

The objective of the study was to verify the influence of climatic seasons on botanical composition and on available dry matter of grasslands in the physiographic region known as Serra do Sudeste (Southeastern Range), RS, Brazil. The climate is Cfb according to Koëppen`s classification. The experiment was conducted in two natural grasslands submitted to two pre-experimental stocking rates [area 1: 0.65 AU ha-1 (normal grazing); area 2: over 2.0 AU ha-1 (overgrazing); AU: Animal Unit = 500kg live weight]. Stocking rates throughout the experimental period (June 1996–July 1997) were 0.4 and 0.65 AU ha-1, over winter and the other seasons, respectively. The evaluations were made at the end of each season, using the Botanal package. There was a short forage supply over late autumn/early winter. The two species groupings warm-season grasses (70–78% of total dry matter) and broad leaves (18– 24%) were the main components of the vegetation in the lower layer of the two grasslands. Warm-season grasses had their participation slightly increased during the warmer seasons. The pre-experimental overgrazing of area 2 favoured the contribution of the minor groups cool-season grasses, warm-season legumes and cool-season legumes

Rotação de culturas. XXIV. Efeito das culturas de inverno sobre o rendimento de grãos e sobre algumas características agronômicas de plantas de soja, num período de cinco anos.

bitstream/item/133651/1/ID12356-1988-1989sojaresultados-p100-115.pdfTrabalho apresentado na XVII Reunião de Pesquisa de Soja da Região Sul, Porto Alegre, 1989

Dynamic fluctuations of elastic lines in random environments

We study the fluctuations of the two-time dependent global roughness of finite size elastic lines in a quenched random environment. We propose a scaling form for the roughness distribution function that accounts for the two-time, temperature, and size dependence. At high temperature and in the final stationary regime before saturation the fluctuations are as the ones of the Edwards-Wilkinson interface evolving from typical initial conditions. We analyze the variation of the scaling function within the aging regime and with the distance from saturation. We speculate on the relevance of our results to describe the fluctuations of other non-equilibrium systems such as models at criticality.Comment: 7 pages, 3 figure