Novel foci of Dermacentor reticulatus ticks infected with Babesia canis and Babesia caballi in the Netherlands and in Belgium

BACKGROUND : Autochthonous populations of Dermacentor reticulatus ticks in the Netherlands were discovered after fatal cases of babesiosis occurred in resident dogs in 2004. The presence of D. reticulatus in the Netherlands has also linked with the emergence of piroplasmosis in the resident horse population. The aim of this study was to put together results of continued surveillance of field sites and hosts for this tick in the Netherlands and also in Belgium and determine their infection status for Babesia and Theileria species. METHODS : Ticks were collected from the vegetation at 11 locations between 2011 and 2013. D. reticulatus ticks were also collected from different hosts between 2007 and 2013. Ticks were screened by PCR and reverse line blot (RLB). RESULTS : A total of 1368 D. reticulatus ticks were collected from 4 previously known field locations and from 5 new locations in the Netherlands and from 2 sites in Belgium (one old and one new location). A total of 855 ticks collected from 8 locations in the Netherlands and 2 locations in Belgium were tested. Fourteen ticks (1,64%) collected at 4 field locations (Dintelse Gorzen, Rozenburg, Slikken van de Heen and St. Philipsland) were positive for Babesia canis, whereas two ticks were positive for Babesia caballi, one tick in the Dintelse Gorzen in the Netherlands and one tick was found positive in De Panne in Belgium. A further 1092 D. reticulatus ticks were collected between 2007 and 2013 from 40 dogs (132 ticks), two ticks from two humans, 51 ticks from 15 horses, two ticks from two cats, one tick from a roe deer, whereas most ticks (904) were collected from cattle (n = 25). Ticks were found throughout the year on dogs in nearly all provinces of the Netherlands. None of the ticks collected from these hosts were infected. CONCLUSIONS : D. reticulatus is continuing its spread into novel areas. The finding that some autochthonous ticks are infected with B. canis and B. caballi poses a threat to the resident dog and horse population and justifies year-round tick control measures.Prof. Frederic Beugnet and Dr. Fabien Danlois of Merial are thanked for their continued interest and financial support for this study.http://www.parasitesandvectors.comam201

Endosonography With or Without Confirmatory Mediastinoscopy for Resectable Lung Cancer:A Randomized Clinical Trial

PURPOSE:Resectable non-small-cell lung cancer (NSCLC) with a high probability of mediastinal nodal involvement requires mediastinal staging by endosonography and, in the absence of nodal metastases, confirmatory mediastinoscopy according to current guidelines. However, randomized data regarding immediate lung tumor resection after systematic endosonography versus additional confirmatory mediastinoscopy before resection are lacking.METHODS:Patients with (suspected) resectable NSCLC and an indication for mediastinal staging after negative systematic endosonography were randomly assigned to immediate lung tumor resection or confirmatory mediastinoscopy followed by tumor resection. The primary outcome in this noninferiority trial (noninferiority margin of 8% that previously showed to not compromise survival, Pnoninferior &lt;.0250) was the presence of unforeseen N2 disease after tumor resection with lymph node dissection. Secondary outcomes were 30-day major morbidity and mortality.RESULTS:Between July 17, 2017, and October 5, 2020, 360 patients were randomly assigned, 178 to immediate lung tumor resection (seven dropouts) and 182 to confirmatory mediastinoscopy first (seven dropouts before and six after mediastinoscopy). Mediastinoscopy detected metastases in 8.0% (14/175; 95% CI, 4.8 to 13.0) of patients. Unforeseen N2 rate after immediate resection (8.8%) was noninferior compared with mediastinoscopy first (7.7%) in both intention-to-treat (Δ, 1.03%; UL 95% CIΔ, 7.2%; Pnoninferior =.0144) and per-protocol analyses (Δ, 0.83%; UL 95% CIΔ, 7.3%; Pnoninferior =.0157). Major morbidity and 30-day mortality was 12.9% after immediate resection versus 15.4% after mediastinoscopy first (P =.4940).CONCLUSION:On the basis of our chosen noninferiority margin in the rate of unforeseen N2, confirmatory mediastinoscopy after negative systematic endosonography can be omitted in patients with resectable NSCLC and an indication for mediastinal staging.</p

Physiological and psychoacoustical correlates of perceiving natural and modified speech

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Psychoacoustical evaluation of the pitch synchronous overlap-and-add speech waveform manipulation technique using single formant stimuli

This article presents two experiments dealing with a psychoacoustical evaluation of the pitchsynchronous overlap-and-add ͑PSOLA͒ technique. This technique has been developed for modification of duration and fundamental frequency of speech and is based on simple waveform manipulations. Both experiments were aimed at deriving the sensitivity of the auditory system to the basic distortions introduced by PSOLA. In experiment I, manipulation of fundamental frequency was applied to synthetic single-formant stimuli under minimal stimulus uncertainty, level roving, and formant-frequency roving. In experiment II, the influence of the positioning of the so-called &apos;&apos;pitch markers&apos;&apos; was studied. Depending on the formant and fundamental frequency, experimental data could be described reasonably well by either a spectral intensity-discrimination model or a temporal model based on detecting changes in modulation of the output of a single auditory filter. Generally, the results were in line with psychoacoustical theory on the auditory processing of resolved and unresolved harmonics

A Semi-Automated Usability Evaluation Framework for Interactive Image Segmentation Systems

For complex segmentation tasks, the achievable accuracy of fully automated systems is inherently limited. Specifically, when a precise segmentation result is desired for a small amount of given data sets, semi-automatic methods exhibit a clear benefit for the user. The optimization of human computer interaction (HCI) is an essential part of interactive image segmentation. Nevertheless, publications introducing novel interactive segmentation systems (ISS) often lack an objective comparison of HCI aspects. It is demonstrated that even when the underlying segmentation algorithm is the same throughout interactive prototypes, their user experience may vary substantially. As a result, users prefer simple interfaces as well as a considerable degree of freedom to control each iterative step of the segmentation. In this article, an objective method for the comparison of ISS is proposed, based on extensive user studies. A summative qualitative content analysis is conducted via abstraction of visual and verbal feedback given by the participants. A direct assessment of the segmentation system is executed by the users via the system usability scale (SUS) and AttrakDiff-2 questionnaires. Furthermore, an approximation of the findings regarding usability aspects in those studies is introduced, conducted solely from the system-measurable user actions during their usage of interactive segmentation prototypes. The prediction of all questionnaire results has an average relative error of 8.9%, which is close to the expected precision of the questionnaire results themselves. This automated evaluation scheme may significantly reduce the resources necessary to investigate each variation of a prototype’s user interface (UI) features and segmentation methodologies

ICT Based Interventions for Anganwadi Healthcare Workers in Mumbai

Part 9: Field TripsInternational audienceAnganwadi workers [1] form the core of healthcare system for a large section of rural and semi-urban population in India. They provide care for newborn babies and play an important role in immunization programs, besides providing health related information to pregnant women. Traditionally these Anganwadi workers use paper based information leaflets as a part of their job to spread awareness among the people. Although mobile phones have made their inroads into the day to day life of these workers for basic communication (making a call), however it is yet to be seen how a mobile device is being used as a technological aid for their work. There are enormous challenges in addressing these issues especially in developing regions owing to numerous reasons such as illiteracy, cognitive difficulties, cultural norms, collaborations, experience and exposure, motivation, power relations, and social standing [2]. The purpose of this field visit would be to enquire the role of mobile devices in their day-to-day work; and if being used as a technological intervention, then in what manner and form is it being used? The methodology used to conduct the study would involve contextual enquiry, open-ended interviews and observing the Anganwadi workers using ICT solutions and other informational artefacts