O aumento da frequência de salmonella clínica Brasil.

A atividade suinícola é um expoente econômico e cultural que gera renda para famílias de agricultores, emprego direto nas agroindústrias e indireto nas atividades relacionadas, também contribui sobremaneira para o balanço comercial e PIB do país. A manutenção e acesso a mercados exigem a superação de desafios dentre os quais merecem atenção a sustentabilidade das propriedades, o bem estar dos animais, a melhoria da biosseguridade das granjas e o status sanitário do rebanho. O maior obstáculo sanitário deriva do crescente número de animais nos plantéis suinícolas aliado ao reaparecimento de enfermidades de grande impacto na cadeia de produção. Dentre as inúmeras patologias que afetam os suínos, a salmonelose clínica desponta com grande importância nos últimos anos. A contaminação por salmonelas na suinocultura se caracteriza por dois problemas: a presença de sorovares patogênicos, adaptados ao suíno, que provocam gastroenterites e septicemias e a presença de sorovares que não causam doença nos animais, mas são as principais fontes de contaminação das carcaças nos abatedouros e que podem infectar seres humanos. Porém, o foco deste relato é o aumento e caracterização da salmonelose clínica que vem ocorrendo nos últimos anos no Brasil

Is high prevalence of Echinococcus multilocularis in wild and domestic animals associated with disease incidence in humans?

We investigated a focus of highly endemic Echinococcus multilocularis infection to assess persistence of high endemicity in rural rodents, explore potential for parasite transmission to domestic carnivores, and assess (serologically) putative exposure versus infection frequency in inhabitants of the region. From spring 1993 to spring 1998, the prevalence of E. multilocularis in rodents was 9% to 39% for Arvicola terrestris and 10% to 21% for Microtus arvalis. From June 1996 to October 1997, 6 (7%) of 86 feral dogs and 1 of 33 cats living close to the region tested positive for intestinal E. multilocularis infection. Testing included egg detection by coproscopy, antigen detection by enzyme-linked immunosorbent assay (ELISA), and specific parasite DNA amplification by polymerase chain reaction. Thus, the presence of infected domestic carnivores can increase E. multilocularis exposure risk in humans. A seroepidemiologic survey of 2,943 blood donors in the area used specific Em2-ELISA. Comparative statistical analyses of seroprevalence and clinical incidence showed an increase in Em2-seroprevalence from 1986 and 1996-97 but no increase in clinical incidence of alveolar hydatid disease

Salmonella clinical isolates from Brazilian pig herds: genetic relationship and antibiotic resistance profiling.

Abstract In Brazil, since 2011 clinical cases of salmonellosis has been increasing substantially. Nevertheless, few information is available about the antimicrobial profile, distribution, serotypes and genetic relationship among the strains. The objectives of this study were: to identify the Salmonella serotypes, to characterize the in vitro antimicrobial resistance profiles and to determine the genetic relationship of clinical isolates in Brazil. During 2016, clinical isolates of Salmonella (111) from nine States were sent to Embrapa Swine and Poultry for complementary analysis. First, isolates were serotyped by Kauffmann White Scheme. In parallel, the strains were tested against fifteen antimicrobials by disk diffusion method and genotyping was performed by Pulsed Field Gel Electrophoresis (PFGE) using the XbaI restriction enzyme. As expected, the main serovars found were Typhimurium and Choleraesuis. Four strains showed resistance to only one antimicrobial and 76.5% (85/111) were considered multiresistant. The highest level of resistance was found against to tetracycline. More than 80% of the strains were susceptible to fosfomycin, lincomycin/spectinomycin and norfloxacin. It was possible to identify one major Choleraesuis clonal group present in different Brazilian States. Further, several small clonal groups were obtained for Typhimurium. In conclusion, clinical salmonellosis caused by Typhimurium and Choleraesuis is endemic in pig production areas and the majority of the strains are multi-resistantSafePork 2017

An Investigation of Diffuse Interstellar Gas toward a Large, Low Extinction Window into the Inner Galaxy

Halpha and Hbeta spectroscopy with the Wisconsin H-Alpha Mapper (WHAM) reveals a strong concentration of high velocity emission in a ~ 5 by 5 deg area centered near (l.b) = (27,-3), known as the Scutum Cloud. The high velocities imply that we are detecting optical emission from near the plane of the Galaxy out to the tangent point at heliocentric distances of D \gtrsim 6 kpc, assuming the gas participates in circular Galactic rotation. The ratio of the Halpha to Hbeta emission as a function of velocity suggests that dust along these lines of sight produces a total visual extinction of A_v ~ 3 at D ~ 6 kpc. This makes it possible to use optical emission lines to explore the physical conditions of ionized gas in the inner Galaxy. At a Galactocentric distance R_G ~ 4 kpc, for example, we find that the H^+ has an rms midplane density of ~ 1 cm^-3 with a vertical scale height of ~ 300 pc. We also find evidence for an increase in the flux of Lyman continuum photons and an increase in the ratio of ionized to neutral hydrogen toward the inner Galaxy. We have extended the measurements of E(B-V) in this direction to distances far beyond what has been accessible through stellar photometry and find E(B-V)/N_H to be near the local mean of 1.7 x 10^-22 cm^2 mag, with evidence for an increase in this ratio at R_G ~ 4 kpc. Finally, our observations of [NII] 6583, [SII] 6716, and [OIII] 5007 toward the window reveal that in the inner Galaxy the temperature of the gas and the ionization state of oxygen increase with increasing height from the midplane.Comment: ApJ, accepted. 28 pages, 13 figures, 1 tabl

Comparing Galaxy Morphology at Ultraviolet and Optical Wavelengths

We have undertaken an imaging survey of 34 nearby galaxies in far-ultraviolet (FUV, ~1500A) and optical (UBVRI) passbands to characterize galaxy morphology as a function of wavelength. This sample, which includes a range of classical Hubble types from elliptical to irregular with emphasis on spirals at low inclination angle, provides a valuable database for comparison with images of high-z galaxies whose FUV light is redshifted into the optical and near- infrared bands. Ultraviolet data are from the UIT Astro-2 mission. We present images and surface brightness profiles for each galaxy, and we discuss the wavelength-dependence of morphology for different Hubble types in the context of understanding high-z objects. In general, the dominance of young stars in the FUV produces the patchy appearance of a morphological type later than that inferred from optical images. Prominent rings and circumnuclear star formation regions are clearly evident in FUV images of spirals, while bulges, bars, and old, red stellar disks are faint to invisible at these short wavelengths. However, the magnitude of the change in apparent morphology ranges from dramatic in early--type spirals with prominent optical bulges to slight in late-type spirals and irregulars, in which young stars dominate both the UV and optical emission. Starburst galaxies with centrally concentrated, symmetric bursts display an apparent ``E/S0'' structure in the FUV, while starbursts associated with rings or mergers produce a peculiar morphology. We briefly discuss the inadequacy of the optically-defined Hubble sequence to describe FUV galaxy images and estimate morphological k-corrections, and we suggest some directions for future research with this dataset.Comment: Accepted for publication in the ApJS. 15 pages, 17 JPEG figures, 10 GIF figures. Paper and full resolution figures available at http://nedwww.ipac.caltech.edu/level5/Kuchinski/frames.htm

The diagnostic value of liver biopsy

BACKGROUND: Since the introduction of molecular diagnostic tools such as markers for hepatitis C and different autoimmune diseases, liver biopsy is thought to be useful mainly for staging but not for diagnostic purposes. The aim was to review the liver biopsies for 5 years after introduction of testing for hepatitis C, in order to evaluate what diagnostic insights – if any – remain after serologic testing. METHODS: Retrospective review of all liver biopsies performed between 1.1.1995 and 31.12.1999 at an academic outpatient hepatology department. The diagnoses suspected in the biopsy note were compared with the final diagnosis arrived at during a joint meeting with the responsible clinicians and a hepatopathologist. RESULTS: In 365 patients, 411 diagnoses were carried out before biopsy. 84.4 % were confirmed by biopsy but in 8.8 %, 6.8 % and 10.5 % the diagnosis was specified, changed or a diagnosis added, respectively. Additional diagnoses of clinical relevance were unrecognized biliary obstruction and additional alcoholic liver disease in patients with chronic hepatitis C. Liver biopsy led to change in management for 12.1 % of patients. CONCLUSION: Even in the era of advanced virological, immunological and molecular genetic testing, liver biopsy remains a useful diagnostic tool. The yield is particularly high in marker negative patients but also in patients with a clear-cut prebiopsy diagnosis, liver biopsy can lead to changes in patient management

Efficacy and safety of an intravenous monoclonal anti-HBs in chronic hepatitis B patients

Background Aims: In this study the safety and efficacy of a monoclonal anti-HBs, Tuvirumab (Mab), were investigated. Tuvirumab is a human monoclonal antibody recognizing the stable 'a'-determinant of the HBsAg. Methods: We included ten chronic hepatitis B patients: four received monotherapy, and six combination therapy with interferon alpha 2b. Results: Because the development of insoluble [HBsAg-HBsAb] complexes led to adverse events, the Mab dose had to be reduced in seven patients. In nine patients treatment was stopped prematurely because of lack of efficacy, i.e. neutralization of HBsAg in serum. However, temporary HBsAg levels were reduced by at least 50% in all patients; in three patients receiving combination therapy, background levels of HBsAg in serum were reached. A loss of serum HBV-DNA was seen in three patients in the combination group, followed by HBeAg seroconversion in two patients. Conclusions: We conclude that Mab was not effective in achieving primary efficacy as assessed by neutralization of circulating HBsAg. Whether a combination of Mab with an antiviral agent that reduces the HBsAg load - and therefore minimizes the risk of adverse events - may result in clinical efficacy should be investigated