Effect of hydroxybenzoic acids antioxidants on the oxidative stability of sardine oil

The antioxidant capacities of three derivatives of hydroxybenzoic acids (Gentisic acid, protochatechuic acid and vanillic acid) in sardine oil were compared. Peroxide value, conjugated diene value, p-anisidine value and thiobarbituric acid reactive substances (TBARS) value were assessed to determine the oxidative stability provided by these substances to the sardine oil. Results showed that gentisic acid (2,5 dihydroxy benzoic acid) was the most effective of the chosen hydroxybenzoic acids in imparting oxidative stability to the sardine oil. Protochatechuic acid (3,4 dihydroxy benzoic acid) provided relatively less oxidative stability, while vanillic acid had no effect. Results from this work showed that the position of hydroxylation and methyl substitution influences the antioxidant capacity of the molecules in sardine oil. Furthermore, it was found that the extent of oxidative stability conferred by the antioxidants in lipid systems is influenced by several other physical and chemical factors as well

Screening of polymeric membranes for membrane assisted deacidification of sardine oil

The diversification in fish oil use and the need for softer processing demand new oil refining processes. In considering the advantages of membrane technology, three different membranes (polyamide (PA), polytetrafluoroethylene (PTFE) and polyethersulfone (PES)) were used in this particular study. Preliminary results in the separation of free fatty acids (FFA) from glycerides of sardine oil/ethanol mixtures using a single dead end microfiltration mode have been reported here. The influence of experimental factors like pressure and oil/ethanol ratios (w/v) on the permeate flux and the reduction of FFA (%) in the permeate was studied. PTFE membrane showed promising results in terms of residual FFA in permeate (%), % oil loss (15.12%, 5.45%) as compared to PA (20.50%, 6.66%) and PES membranes (20.60%, 8.92%). PA membrane showed a higher flux of 4.4 L/m2 /h, followed by PTFE 3.34 L/m2 /h and PES, 1.19 L/m2 /h at 3.5 bar trans-membrane pressure. These results showed that using PTFE membrane could be an ideal strategy for the membrane assisted deacidification of sardine oil using solvents

Reverse micellar partitioning of Bovine Serum Albumin with novel system

To overcome the difficulties associated with the conventional extraction process like poor selective extraction of biomolecule and scale up of the process, the reverse micellar system consist of AOT/n-heptanol was considered to extract Bovine Serum Albumin (BSA) as a model biomolecule. The maximum forward extraction of BSA from aqueous phase to micelle phase was observed at AOT concentration 160â¯mM, aqueous phase pH value of 4, NaCl concentration 0.8â¯M and 95% back extraction of BSA from micelle phase to stripping phase was obtained at 1â¯M NaCl concentration with the pH of 7.5. HPLC analysis confirmed the stability of BSA during extraction. The size and water content of the reverse micelle was also reported. The obtained results emphasize the application of the AOT/n-heptanol reverse micellar system for the extraction of BSA and may be utilized for the selective extraction of similar hydrophilic proteins from the complex sources. Keywords: AOT, Reverse micellar extraction, Reverse micelles characterization, Bovine Serum Albumi