Probiotic Lactobacillus species Inhibitory Effect on The Growth of Oral Streptococci

Oral and dental infections are among the most prevalent infections of man. All people suffer from dental caries at some phase oftheir life span. The mouth is indeed an important source of infections and poor oral health affects a variety of systemic diseases.The aim of this research is to isolate, identify acid-producing lactic acid bacteria (LAB) from fermented fruits juice and followedby the 16S rRNA gene sequences of the isolated bacterial strains Lactobacillus Plantarum S1, Leuconostoc mesenteroides (SCand PP) and Burkholderia cenocepia NP were compared with reference strain sequences. Oral Streptococci were important inthe etiology of dental caries. We targeted for isolation and biochemical identification of oral bacteria (isolates grown in SMABagar) and screened antibacterial activity (agar well diffusion assay) of Lactobacillus species against oral Streptococci. Two Lactobacillus isolates (S1 and PP) were observed to behave a good antagonistic activity against oral Streptococcus strain withdifferences in the size of inhibition zone (mm). The zone diameter of NP broth against Streptococcus sp. was 10 mm. Oral Streptococci was not inhibited by SC broth. In this study, Leuconostoc mesenteroides (PP) showed the highest inhibition zone(13 mm) against oral Streptococci

Comparative analysis of sugarcane root transcriptome in response to the plant growth-promoting Burkholderia anthina MYSP113.

The diazotrophic Burkholderia anthina MYSP113 is a vital plant growth-promoting bacteria and sugarcane root association. The present study based on a detailed analysis of sugarcane root transcriptome by using the HiSeq-Illumina platform in response to the strain MYSP113. The bacterium was initially isolated from the rhizosphere of sugarcane. To better understand biological, cellular, and molecular mechanisms, a de novo transcriptomic assembly of sugarcane root was performed. HiSeq-Illumina platformwas employed for the sequencing of an overall of 16 libraries at a 2×100 bp configuration. Differentially expressed genes (DEGs) analysis identified altered gene expression in 370 genes (total of 199 up-regulated genes and 171 down-regulated genes). Deciphering the huge datasets, concerning the functioning and production of biological systems, a high throughput genome sequencing analysis was attempted with Gene ontology functional analyses and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. The report revealed a total of 148930 unigenes. 70414 (47.28%) of them were annotated successfully to Gene Ontology (GO) terms. 774 at 45 days, 4985 of 30 days and 15 days of 6846 terms were significantly regulated. GO analysis revealed that many genes involved in the metabolic, oxidation-reduction process and biological regulatory processes in response to strain MYSP113 and significantly enriched as compare to the control. Moreover, KEGG enriched results show that differentially expressed genes were classified into different pathway categories involved in various processes, such as nitrogen metabolism, plant hormone signal transduction, etc. The sample correlation analyses could help examine the similarity at the gene expression level. The reliability of the observed differential gene expression patterns was validated with quantitative real-time PCR (qRT-PCR). Additionally, plant enzymes activities such as peroxidase and superoxide dismutase were significantly increased in plant roots after the inoculation of strain MYSP113. The results of the research may help in understanding the plant growth-promoting rhizobacteria and plant interaction