OPTIMIZATION OF ELECTROPORATION MEDIATED TRANSFORMATION OF LACTOBACILLUS PLANTARUM FOR INDUSTRIAL EXPLOITATION

Objective: The objective of our study was to evaluate different established electrotransformation protocols for their suitability on Lactobacillus plantarum.Methods: We tested strategies of electrical parameters in addition to already published electroporation methods proven to be successful in other Lactobacillus strains.Results: Our strain differed highly in transformation efficiencies and optimal growth conditions. Among the various methods performed, we observed high-efficiency protocol yielded 8X106 transformants, and the optimum electroporation parameter was found to be at 1500V.Conclusion: Independent of electroporation method Lactobacillus plantarum strain remained reluctant to high-efficiency transformation protocol

Study of Intermolecular Interaction in Binary Mixtures of Paraanisaldehyde with Chlorobenzene, Toluene and Acetone at 313.15K

Abstract The ultrasonic velocity, density and viscosity at 313.15

Isolation, Screening and molecular characterization of bio surfactant producing microbes from hydrocarbon polluted soil

Biosurfactants are ampliphlic compounds which can reduce the surface and interfacial tension between the compounds and have multiuses in various industries and agriculture. The isolated lipopeptides and lipoprotein producing microbes are useful in removal of heavy metals from industrial effluents using bioremediation, pesticides and hydrocarbon contaminated sites. Lipopeptides and lipoproteins used as bio-control agent to protect plant against different diseases, leads in increases crop yield. The objective was to isolate potential bio surfactant producing microbes from hydrocarbons contaminated soil. Initially 21 microbes were isolated amidst them ten bio surfactant producers were screened based on the surface tension, emulsification index, drop collapse method and oil displacement method. The isolate KLEF21 was the most successful bio surfactant producer as it showed the minimum surface tension (32.12 dyne/cm) with an emulsification index of 68.97% and oil displacement activity 3.5 mm. The microbe was identified based on the partial sequence of 16S rRNA and it belongs to Alcaligenes and was indicated as Alcaligenes sp.KLEF21.The stain was also competent of employ polycyclic aromatic hydrocarbon composed of three fused benzene rings as the highest microbes biomass was produced by this strain in polycyclic aromatic hydrocarbon alter MSM media compared to other isolates

Evaluation of Biosurfactant Property within Plant Probiotic Species

The present study focusses on proving the biosurfactant activity of the 7 isolated bacterial strains. Plants could not absorb enough nutrients as a result of contamination in the soil. This contaminants can be chemical pollutants or oil spillage. By considering the earlier potential applications of biosurfactants, this research was aimed to prove the role of a plant probiotic bacteria as oil degrading bacteria. In order to prove this approach, many screening methods were performed such as drop collapse, oil spreading (mm), emulsification stability testing by calculating emulsification index (E24%), penetration assay etc. Among the 7 selected isolates, isolate KL-015 have shown positive results with all the biosurfactant screening analysis. A unique role was proven by KL-011 isolate as it has shown a high oil degrading zone, i.e., 0.6 mm in diameter as well as the formation of more foam layer or emulsified layer with the emulsification index of 17.39% which indicates its role in having a biosurfactant molecule within it