Anticancer activity and phytochemical composition of wild Gundelia tournefortii

Artichoke‑like wild thistles are often used in Palestinian cuisine. One of the most commercially recognized species of these wild edible thistles is Gundelia tournefortii, a common plant in the Mediterranean region. G. tournefortii, or ‘Akoob’ in Arabic, remains uncultivated, harvested wild by local populations and considered highly valuable due to its reputed health benefits. The present study aimed to investigate the anticancer effects of G. tournefortii on the human colon carcinoma HCT‑116 cell line. Methanol and hexane extracts were identified to exert considerable antitumor activity against the HCT‑116 cancer cell line, while the aqueous extract was inactive. The phytochemical profiles of the methanol and hexane extracts were investigated using gas chromatography‑mass spectrometry. A total of 6 of the 27 natural compounds identified, including sitosterol, stigmasterol, lupeol, gitoxigenin, α‑amyrin and artemisinin, have been previously validated as being active against cancerous cells. Therefore, the presence of these phytochemicals in G. tournefortii is of importance in its role in preventing and treating cancer. according to different localities. In Palestinian traditional medicine and ethno‑botany, this plant is believed to possess nutritive and curing benefits for diabetes, epilepsy, stomach and intestinal diseases (3,4). According to the literature, it has been validated to exert antioxidant, hepatoprotective and antibacterial effects (1,5). A previous study (2) conducted among Palestinians eating Akoob on a regular basis revealed a consensus belief of its capability to prevent and cure cancer. However, an intensive search of PubMed (using the terms gundelia tournefortii and cancer; on 12th September 2016) indicates that there is no single study on the effects of G. tournefortii extracts against cancer. The present study aimed to investigate the anticancer effects of G. tournefortii on the human colon cancer HCT‑116 cell line. Gas chromatography‑mass spectrometry (GC‑MS) was utilized to explore the potential phytochemicals responsible for the anticancer activity. A total of 27 constituents were identified in G. tournefortii, of which 6 phytochemicals, including sitosterol, stigmasterol, lupeol, gitoxigenin, α‑amyrin and artemisinin have been demonstrated to exhibit anticancer activities. To the best of our knowledge, the present study was the first to investigate the potential benefits of consuming wild edible G. tournefortii for cancer, and to analyze the phytochemical contents known for their anticancer effects.The present study was supported by unrestricted grants from Al‑Qasemi Research Foundation (Baqa‑El‑Gharbia, Israel; grant no. 898002) and Ministry of Science, Technology and Space, Israel

Integrating PhET Simulations into Elementary Science Education: A Qualitative Analysis

This research delved into the integration of PhET simulations in elementary science education, specifically aimed at Grade 3 students. The primary objective was to evaluate how the use of these digital simulations influenced students’ conceiving of scientific concepts, focusing on “States of M1atter and Phase Changes” and “Solubility and Saturation”. Employing a qualitative research approach, the study observed 19 students who worked in pairs and trios as they engaged with PhET simulations to explore assigned science topics and address related questions. The observations centered on tracking students’ interactions with simulations and their progression through different knowledge phases. We used deductive and inductive content analysis to analyze the transcripts of the observation. The findings reveal that in the “Remembering” phase, students demonstrated a tendency to relate personal experiences to simulations, underscoring real-life context’s role in learning. The “Understanding” phase highlighted how PhET simulations facilitated deeper comprehension, with students making insightful observations. Additionally, the “Application” phase showcased the effective translation of simulation-derived knowledge into practical scenarios, bridging theoretical and real-world understanding. Students’ use of high-order thinking skills, at the analysis, evaluation, and creative phases, showed that simulations supported Grade 3 students in their learning processes of scientific concepts. The research underscores the efficacy of integrating PhET simulations into elementary science education, enhancing students’ knowledge by promoting active engagement and problem-solving skills. Integrating simulations into teaching methodologies emerges as a promising avenue to nurture scientific expertise and holistic understanding among elementary school students

The Feasibility of Telemedicine in the Implementation and Management of Therapeutic Hypothermia for Infants with Neonatal Hypoxic-Ischemic Encephalopathy in a Resource-Limited Country

Background Telemedicine is widely used in neonatal services in developed countries, though its outcomes in low- and middle-income countries are controversial. Lack of expertise and/or facilities, however, has limited its use in developing countries and around areas of military conflicts. We aim to study the implementation and management of therapeutic hypothermia (TH) in infants with hypoxic-ischemic encephalopathy (HIE) with the help of telemedicine in a resource-limited country. Methodology This is a retrospective study, evaluating patients who received TH, guided by telemedicine, through a mobile app (Telegram), an application that allows sharing and archiving of information with other beneficial features. We assessed the feasibility of utilizing telemedicine in guiding the application of TH to infants affected with HIE in the North-West of Syria between July 2020 and July 2021. Feasibility was measured by parameters related to the time gaps between initiation of consultation and treatment and clinical short-term outcomes. Results Out of 5,545 newborn infants delivered during the study period, 22 patients were eligible for TH guided by telemedicine. Patients were referred for consultation at a median (interquartile range [IQR]) of 137 (35–165) minutes of life. A median (IQR) of 12 (3–18) minutes elapsed between the call for a consultation and the consultant response and a median (IQR) of 30 (0–42) minutes elapsed between seeking the consultation and the initiation of cooling therapy. Eighteen patients completed cooling for 72 hours. The patients' temperatures were within the target range (33–34°C) most of the time (84.1%). Conclusion Telemedicine is a feasible method to guide the implementation TH for HIE in resource-limited areas. The short-term success rate is relatively high; however, further studies with a larger population are needed to confirm these findings

Determination of antibodies to Caprine arthritis encephalitis virus in goats and sheep in some localities in Sudan

Objective: Caprine arthritis encephalitis (CAE) infection is existing worldwide causing economic loss in goat production. This work was conducted to investigate the prevalence of CAE in local and foreign goat breeds and sheep in different localities in Sudan. Materials and methods: A total of 368 sera samples were prepared; among these, 112 were collected from cross (n=39) and foreign (n=73) breeds of goat in Khartoum state, equal number of sera (n=23) were collected from goats and sheep from each of River Nile, Gedarif, Sinnar and North Kordofan states. Eighteen cross bred goats were tested from River Nile state. From foreign bred goats, 28 and 26 sera were collected from Gazira and Northern states, respectively. The collected sera were examined for the presence of antibodies to CAE virus using Enzymed-Linked Immunosorbent Assay (ELISA). Results: The overall seroprevalence of CAE was 2.99% (n=11/368). Positive results were found only in 11 of foreign breed of goats (Cyprus shami) collected from Gazira (39.29%; n=11/28). Statistically, there was an association between breed and location (P=0.001 and P=0.671, respectively). Conclusion: The CAE virus infection exists only in foreign goat breeds. Gazira state showed the highest seroprevalence as compared to other states considered in this study. Strict hygienic measures should be adopted to control the disease and to prevent its spread to local breeds. [J Adv Vet Anim Res 2016; 3(3.000): 259-262