Rotating bio-reactor cell culture apparatus

A bioreactor system is described in which a tubular housing contains an internal circularly disposed set of blade members and a central tubular filter all mounted for rotation about a common horizontal axis and each having independent rotational support and rotational drive mechanisms. The housing, blade members and filter preferably are driven at a constant slow speed for placing a fluid culture medium with discrete microbeads and cell cultures in a discrete spatial suspension in the housing. Replacement fluid medium is symmetrically input and fluid medium is symmetrically output from the housing where the input and the output are part of a loop providing a constant or intermittent flow of fluid medium in a closed loop

X-ray and radio prompt emission from a hypernova SN 2002ap

Here we report on combined X-ray and radio observations of SN 2002ap with XMM-Newton ToO observation and GMRT observations aided with VLA published results. In deriving the X-ray flux of SN 2002ap we account for the contribution of a nearby source, found to be present in the pre-SN explosion images obtained with Chandra observatory. We also derive upper limits on mass loss rate from X-ray and radio data. We suggest that the prompt X-ray emission is non-thermal in nature and its is due to the repeated compton boosting of optical photons. We also compare SN's early radiospheric properties with two other SNe at the same epoch.Comment: 4 pages, 5 figures. Uses espcrc2.sty. To appear in proceedings of symposium on X-ray astronomy "The Restless High-Energy Universe", May 2003, Amsterdam, The Netherlands, eds. E.P.J. van den Heuvel, J.J.M. in 't Zand, and R.A.M.J. Wijer

Method for culturing mammalian cells in a perfused bioreactor

A bio-reactor system wherein a tubular housing contains an internal circularly disposed set of blade members and a central tubular filter all mounted for rotation about a common horizontal axis and each having independent rotational support and rotational drive mechanisms. The housing, blade members and filter preferably are driven at a constant slow speed for placing a fluid culture medium with discrete microbeads and cell cultures in a discrete spatial suspension in the housing. Replacement fluid medium is symmetrically input and fluid medium is symmetrically output from the housing where the input and the output are part of a loop providing a constant or intermittent flow of fluid medium in a closed loop

The late time radio emission from SN 1993J at meter wavelengths

We present the investigations of SN 1993J using low frequency observations with the Giant Meterwave Radio Telescope. We analyze the light curves of SN 1993J at 1420, 610, 325 and 243 MHz during $7.5-10$ years since explosion.The supernova has become optically thin early on in the 1420 MHz and 610 MHz bands while it has only recently entered the optically thin phase in the 325 MHz band. The radio light curve in the 235 MHz band is more or less flat. This indicates that the supernova is undergoing a transition from an optically thick to optically thin limit in this frequency band. In addition, we analyze the SN radio spectra at five epochs on day 3000, 3200, 3266, 3460 and 3730 since explosion. Day 3200 spectrum shows a synchrotron cooling break. SN 1993J is the only young supernova for which the magnetic field and the size of the radio emitting region are determined through unrelated methods. Thus the mechanism that controls the evolution of the radio spectra can be identified. We suggest that at all epochs, the synchrotron self absorption mechanism is primarily responsible for the turn-over in the spectra. Light curve models based on free free absorption in homogeneous or inhomogeneous media at high frequencies overpredict the flux densities at low frequencies. The discrepancy is increasingly larger at lower and lower frequencies. We suggest that an extra opacity, sensitively dependent on frequency, is likely to account for the difference at lower frequencies. The evolution of the magnetic field (determined from synchrotron self absorption turn-over) is roughly consistent with $B \propto t^{-1}$. Radio spectral index in the optically thin part evolves from $\alpha \sim 0.8-1.0$ at few tens of days to $\sim 0.6$ in about 10 years.Comment: 37 pages, 9 figures in LaTex; scheduled for ApJ 10 September 2004, v612 issue; send comments to: [email protected]

Experimental measurement of the orbital paths of particles sedimenting within a rotating viscous fluid as influenced by gravity

Measurements were taken of the path of a simulated typical tissue segment or 'particle' within a rotating fluid as a function of gravitational strength, fluid rotation rate, particle sedimentation rate, and particle initial position. Parameters were examined within the useful range for tissue culture in the NASA rotating wall culture vessels. The particle moves along a nearly circular path through the fluid (as observed from the rotating reference frame of the fluid) at the same speed as its linear terminal sedimentation speed for the external gravitational field. This gravitationally induced motion causes an increasing deviation of the particle from its original position within the fluid for a decreased rotational rate, for a more rapidly sedimenting particle, and for an increased gravitational strength. Under low gravity conditions (less than 0.1 G), the particle's motion through the fluid and its deviation from its original position become negligible. Under unit gravity conditions, large distortions (greater than 0.25 inch) occur even for particles of slow sedimentation rate (less than 1.0 cm/sec). The particle's motion is nearly independent of the particle's initial position. Comparison with mathematically predicted particle paths show that a significant error in the mathematically predicted path occurs for large particle deviations. This results from a geometric approximation and numerically accumulating error in the mathematical technique

Squeezars: Tidally powered stars orbiting a massive black hole

We propose that there exists a class of transient sources, "squeezars", which are stars caught in highly eccentric orbits around a massive (m<10^8 Mo) black hole (MBH), whose atypically high luminosity (up to a significant fraction of their Eddington luminosity) is powered by tidal interactions with the MBH. Their existence follows from the presence of a mass sink, the MBH, in the galactic center, which drives a flow of stars into nearly radial orbits to replace those it has destroyed. We consider two limits for the stellar response to tidal heating: surface heating with radiative cooling ("hot squeezars") and bulk heating with adiabatic expansion ("cold squeezars"), and calculate the evolution of the squeezar orbit, size, luminosity and effective temperature. The squeezar formation rate is only ~0.05 that of tidal disruption flares, but squeezar lifetimes are many orders of magnitude longer, and so future observations of squeezars in nearby galaxies can probe the tidal process that feeds MBHs and the effects of extreme tides on stars. The mean number of squeezars orbiting the Galactic MBH is estimated at 0.1-1.Comment: ApJ Lett. accepted. 4 pp. 1 fi

Cold gas and star formation in a merging galaxy sequence

We explore the evolution of the cold gas and star-formation activity during galaxy interactions, using a merging galaxy sequence comprising both pre- and post-mergers. Data for this study come from the literature but supplemented by new radio observations presented here. Firstly, we confirm that the star-formation efficiency (SFE) increases close to nuclear coalescence. At post-merger stages there is evidence that the SFE declines to values typical of ellipticals. This trend can be attributed to M(H_2) depletion due to interaction induced star-formation. However, there is significant scatter, likely to arise from differences in the interaction details of individual systems. Secondly, we find that the central molecular hydrogen surface density, increases close to the final stages of the merging of the two nuclei. Such a trend is also predicted by numerical simulations. Furthermore, there is evidence for a decreasing fraction of cold gas mass from early interacting systems to merger remnants, attributed to gas conversion into other forms. The evolution of the total-radio to blue-band luminosity ratio, reflecting the disk+nucleus star-formation activity, is also investigated. Although this ratio is on average higher than that of isolated spirals, we find a marginal increase along the merging sequence, attributed to the relative insensitivity of disk star-formation to interactions. However, a similar result is also obtained for the nuclear radio emission, although galaxy interactions are believed to significantly affect the activity in the central galaxy regions. Finally, we find that the FIR--radio flux ratio distribution of interacting galaxies is consistent with star-formation being the main energising source.Comment: 18 pages, 17 figures, accepted for publication in MNRA