Epithelium detection and cervical intraepithelial neoplasia classification in digitized histology images

“Cervical cancer is one of the most deadly cancers faced by women. It is the second leading cause of cancer death in women aged 20 to 39 years. In order to detect cancer at early stages, pathologists analyze the epithelium region from the cervical histology images. These histology images have a pre-cervical cancer condition called cervical intraepithelial neoplasia (CIN) determined by pathologists. This study deals with automating the process of epithelium detection and epithelium CIN classification in digitized histology images. For epithelium detection, the objective is to detect epithelium regions in microscopy images from non-epithelium regions and background. convolutional neural networks, both shallow and deep networks are used for epithelium detection. The highest epithelium detection accuracy of 98.84% is obtained using transfer learning on VGG-19 architecture, pre-trained on the ImageNet dataset. For CIN classification, the epithelium region is divided into 5 segments along the medial axis and patches from each segment were used for training the deep learning model. Vertical segment level classification probabilities from deep learning model are obtained and further classified using SVM, LDA, MLP, logistic and RF classifiers. The highest image level accuracy obtained is 77.27% for MLP classifier using voting”--Abstract, page iii

Automated cervical digitized histology whole-slide image analysis toolbox

Background: Cervical intraepithelial neoplasia (CIN) is regarded as a potential precancerous state of the uterine cervix. Timely and appropriate early treatment of CIN can help reduce cervical cancer mortality. Accurate estimation of CIN grade correlated with human papillomavirus type, which is the primary cause of the disease, helps determine the patient's risk for developing the disease. Colposcopy is used to select women for biopsy. Expert pathologists examine the biopsied cervical epithelial tissue under a microscope. The examination can take a long time and is prone to error and often results in high inter-and intra-observer variability in outcomes. Methodology: We propose a novel image analysis toolbox that can automate CIN diagnosis using whole slide image (digitized biopsies) of cervical tissue samples. The toolbox is built as a four-step deep learning model that detects the epithelium regions, segments the detected epithelial portions, analyzes local vertical segment regions, and finally classifies each epithelium block with localized attention. We propose an epithelium detection network in this study and make use of our earlier research on epithelium segmentation and CIN classification to complete the design of the end-to-end CIN diagnosis toolbox. Results: The results show that automated epithelium detection and segmentation for CIN classification yields comparable results to manually segmented epithelium CIN classification. Conclusion: This highlights the potential as a tool for automated digitized histology slide image analysis to assist expert pathologists