4 research outputs found
GLADS: A gel-less approach for detection of STMS markers in wheat and rice.
- Author
- Publication venue
- 'Public Library of Science (PLoS)'
- Publication date
- 01/01/2019
- Field of study
Sequence tagged microsatellite site (STMS) are useful PCR based DNA markers. Wide genome coverage, high polymorphic index and co-dominant nature make STMS a preferred choice for marker assisted selection (MAS), genetic diversity analysis, linkage mapping, seed genetic purity analysis etc. Routine STMS analysis involving low-throughput, laborious and time-consuming polyacrylamide/agarose gels often limit their full utility in crop breeding experiments that involve large populations. Therefore, convenient, gel-less marker detection methods are highly desirable for STMS markers. The present study demonstrated the utility of SYBR Green dye based melt-profiling as a simple and convenient gel-less approach for detection of STMS markers (referred to as GLADS) in bread wheat and rice. The method involves use of SYBR Green dye during PCR amplification (or post-PCR) of STMS markers followed by generation of a melt-profile using controlled temperature ramp rate. The STMS amplicons yielded characteristic melt-profiles with differences in melting temperature (Tm) and profile shape. These characteristic features enabled melt-profile based detection and differentiation of STMS markers/alleles in a gel-less manner. The melt-profile approach allowed assessment of the specificity of the PCR assay unlike the end-point signal detection assays. The method also allowed multiplexing of two STMS markers with non-overlapping melt-profiles. In principle, the approach can be effectively used in any crop for STMS marker analysis. This SYBR Green melt-profiling based GLADS approach offers a convenient, low-cost (20-51%) and time-saving alternative for STMS marker detection that can reduce dependence on gel-based detection, and exposure to toxic chemicals
GLADS: A gel-less approach for detection of STMS markers in wheat and rice
- Author
- A Varga
- A Xanthopoulou
- AE Croxford
- Ajay Saini
- B Das
- BC Collard
- BD Singh
- Bikram Kishore Das
- BK Das
- C Dong
- C Gachon
- C Kuleung
- CA Heid
- CMS Mienie
- Deepak Sharma
- DF Calderini
- DJ Somers
- E Arikawa
- E Paux
- G Distefano
- G Vishwakarma
- Gautam Vishwakarma
- H Zipper
- HS Randhawa
- I Baris
- I Ganopoulos
- I Ganopoulos
- I Orsi
- I Simko
- J Cheng
- J Li
- J Pallavi
- Jianyu An
- John Wiley & Sons Ltd
- K Semagn
- KM Ririe
- LR Vemireddy
- LS Kumar
- M Agarwal
- M Bagge
- M Helguera
- M Schuelke
- MJ Giménez
- MJ Hayden
- MLC Vieira
- MS Röder
- N Appleby
- N Eswaran
- N Meyer
- NA Barkley
- Niranjan Baisakh
- NJ Gibson
- P Hernández
- Parmeshwar Kumar Sahu
- PK Gupta
- PK Gupta
- PT Monis
- R Appels
- R Kalendar
- R Lipsky
- R Sánchez-Pérez
- R Varshney
- Ratan Tiwari
- Ravi Prakash Sanyal
- Ravi Raj Singh Patel
- RK Varshney
- S Ashkani
- S Germer
- S Jaiswal
- S Periyannan
- S Taheri
- SR McCouch
- TJ Tsilo
- W Li
- W Ma
- W Ma
- W-C Zhou
- Y Huang
- Z Yan
- Publication venue
- 'Public Library of Science (PLoS)'
- Publication date
- Field of study
Capitalism and the Common Man: Peasants and Petty Production in Africa and South Asia
- Author
- Adnan Shapan
- Agarwal Bina
- Altvater Elmar
- Aryama Thorat S.
- Basile E.
- Bernstein Henry
- Bernstein Henry
- Bharadwaj Krishna
- Breman Jan
- Breman Jan
- Breman Jan
- Byres Terence J.
- Cadene Philippe
- Caille Alain
- Chatterjee Partha
- Contractor Qudisya
- Da Corta Lucia
- Da Corta Lucia
- Djurfelt Goran
- Ellis Frank
- Finer Catherine J.
- Firdos S.
- Garikipati Supriya
- GoI (Government of India)
- Gooptu Nandini
- Guerin Isabelle
- Harriss Barbara
- Harriss John
- Harriss John
- Harriss John
- Harriss John
- Harriss John
- Harriss John
- Harriss-White Barbara
- Harriss-White Barbara
- Harriss-White Barbara
- Harriss-White Barbara
- Harriss-White Barbara
- Harriss-White Barbara
- Harriss-White Barbara
- Harriss-White Barbara
- Harriss-White Barbara
- Hart Keith
- Hasan Zoya
- Hensman Rohini
- Huws Ursula
- Idahosa P.L.E.
- Janakarajan S.
- Jayaraj D.
- Jha P.S.
- Jhabvala R.
- Kannan K.P.
- Kanungo Pralaya
- Kaviraj Sudipta
- Khan Mushtaq
- Lenin V.I.
- Lerche Jens
- Leys Colin
- Leys Colin
- Mahendra Dev S.
- Marshall T.H.
- Mellor John W.
- Mitra Subrata
- Mukherjee-Reed Ananya
- Narain Dharam
- NCEUS (National Commission for Enterprises in the Unorganised Sector)
- NCEUS (National Commission for Enterprises in the Unorganised Sector)
- NCEUS (National Commission for Enterprises in the Unorganised Sector)
- Olsen Wendy
- Omvedt Gail
- Patnaik Utsa
- Patnaik Utsa
- Perelman Michael
- Prakash Aseem
- Ramachandran V.K.
- Raman Ravi
- Rashid Shahidur
- Reddy D. Narshima
- Rogaly Ben
- Ruthven O.
- Salam Umar
- Sankaran K.
- Sankaran K.
- Sankaran K.
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- Shah Alpa
- Shah Alpa
- Sinha Anushree
- Srinivasan Kalyanaraman
- Taussig Michael
- Vidal John
- Williams Gavin
- Williams Gavin
- Williams Gavin
- World Bank
- Publication venue
- 'SAGE Publications'
- Publication date
- Field of study
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- Tianhui Hu
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- Tomohiko J. Itoh
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- Tomoyuki Iwabuchi
- Tomoyuki Nishida
- Toshihiko Ogihara
- Toshio Yanagida
- Tsuguchika Kaminuma
- Tudor Savopol
- Tudor Savopol
- U. Haldar
- U. Haldar
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- Uddhavesh B. Sonavane
- Uma Singh
- Umamaheswar Duvvuri
- Umesh Varshney
- Usha B. Nair
- Usha B. Nair
- V. A. Fedin
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- V. A. Kratasyuk
- V. A. Pashinskaya
- V. A. Pashinskaya
- V. Arakelyan
- V. Arrigler
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- V. I. Ivanov
- V. Jazbinšek
- V. K. Koltover
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- V. Kabaleeswaran
- V. Kiessling
- V. Kothekar
- V. Kothekar
- V. Kulchitsky
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- V. Laurent
- V. M. Mazhul
- V. M. Mazhul
- V. Manko
- V. Martorana
- V. Martorana
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- V. Mohan Kumar
- V. N. Shikhov
- V. N. Shilov
- V. Nastasa
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- V. P. Skulachev
- V. R. Srinivas
- V. Rani Parvathy
- V. S. Chauhan
- V. S. Chubanov
- V. S. Shelkovsky
- V. V. Adamovich
- V. V. Rosin
- V. Villari
- Vasantha Pattabhi
- Vera Pingoud
- Vettai S. Ananthanaravanan
- Vettai S. Ananthanarayanan
- Victor Muñoz
- Vidva Raghunathan
- Vikas Chandra
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- Vinod Bhakuni
- Vitaly K. Koltover
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- W. A. Varanda
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- W. Seidenbusch
- Wang Bao Han
- Wang Jushuo
- Wang Zhi Xin
- Wei-ying Cheng
- Wen-rui Chang
- Werner Kühlbrandt
- William A. Eaton
- Wolfgang Höhne
- Wolfgang Jahnke
- Wolfgang Wende
- Xiao-jian Hu
- Xun Shen
- Y-M. Zheng
- Y. -H. Sanejouand
- Y. Blouquit
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- Y. Lin
- Y. M. Allakhverdieva
- Y. Nakato
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- Y. Rubin
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- Y. Suezaki
- Y. U. Sasidhar
- Ya. I. Estrin
- Yasuhiro Kumaki
- Yasunori Yokoyama
- Yogesh Kumar
- Yoichi Hayakawa
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- Yoshitoshi
- Yoshiyuki Amemiya
- Yosiyuki Sowa
- Yu. Babayan
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- Yu. S. Babayan
- Yuan Zheng
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- Yuriko Yamagata
- Yuriko Yamagata
- Yusuke Iguchi
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- Z. Guttenberg
- Z. Lobo
- Z. S. Várkonyi
- Z. Stolič
- Z. Tronteli
- Zeno Földes-Papp
- Zhao Nanming
- Zhi-hong Zhang
- Zs. Várkonyi
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- Éric Quinjou
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