Virulence of Newcastle disease virus is determined by the cleavage site of the fusion protein and by both the stem region and globular head of the haemagglutinin-neuraminidase protein

Virulence of Newcastle disease virus (NDV) is mainly determined by the amino acid sequence surrounding the fusion (F) protein cleavage site, since host proteases that cleave the F protein of virulent strains are present in more tissues than those that cleave the F protein of non-virulent strains. Nevertheless, comparison of NDV strains that carry exactly the same F protein cleavage site shows that significant differences in virulence still exist. For instance, virulent field strain Herts/33 with the F cleavage site 112RRQRRF117 had an intracerebral pathogenicity index of 1.88 compared with 1.28 for strain NDFLtag, which has the same cleavage site. This implies that additional factors contribute to virulence. After generating an infectious clone of Herts/33 (FL-Herts), we were able to map the location of additional virulence factors by exchanging sequences between FL-Herts and NDFLtag. The results showed that, in addition to the F protein cleavage site, the haemagglutinin-neuraminidase (HN) protein also contributed to virulence. The effect of the HN protein on virulence was most prominent after intravenous inoculation. Interestingly, both the stem region and the globular head of the HN protein seem to be involved in determining virulenc

Influence of knots and density distribution on compressive strength of wooden foundation piles

This work investigated the influence of knots on the compression strength of wooden foundation piles. The study involved 110 pile segments sawn from 18 spruce and 9 pine piles with a mean diameter of approximately 200 mm, and moisture contents above fiber saturation. The mechanical properties were determined performing both full-scale compression tests on pile segments, and small-scale experiments on discs sawn from selected segments, considering samples with and without knots. A knot ratio (KR) was defined analysing the knots layout of each wooden pile, and evaluating how the compressive strength was influenced by size, number and layout of knots. As final step, a prediction model was implemented based on the dry density and KR of wooden piles, to estimate the influence of knots on their compressive strength.Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Bio-based Structures & Material

An economic method to determine the strength class of wood species

Civil Engineering and Geoscience

An integral approach for the assessment of timber pile foundations

Timber pile foundations are widespread in many areas around Europe and North-America. Especially in areas with weak soils, timber pile foundations have been a very good and economic solution. However, aging of the foundations can become a problem, as physical, biological and/or chemical degradation may occur over time. Now, that foundations can be up to 500 years in cities like Venice, Amsterdam, Hamburg, Boston and many others, questions arise about the reliability and which assessment methods can be used in order to estimate the current load carrying capacity and their residual service life. Residual service life is depending both on the time-to-failure behaviour of wood, as well as, the dead and live loads on the piles below buildings, quay walls and bridges. The approach taken integrates degradation models with a reaction kinetics based damage model. For wood that remains consistently below the waterline, the combination of bacterial degradation and long term loading is considered the most important, but degradation by fungi also may occur depending on soil and groundwater conditions. The integral assessment model will function as a tool for repair and maintenance strategies for asset managers and structural engineers.Accepted Author ManuscriptBio-based Structures & Material

Influence of moisture content on the assessment of decay levels by micro-drilling measurements in wooden foundation piles

In the historic city centre of Amsterdam (NL), the most widespread foundation system consists of wooden piles. With the aim of modelling and predicting remaining service life of these foundations and the piles in particular, one of the possible methods for collecting data and monitoring their condition consists of micro-drilling (MD) measurements. This work evaluates the reliability of MD measurements in identifying decayed portions and specific features of wooden foundation piles, considering different moisture content (MC) values. To this end, 24 segments were selected, sawn from wooden piles extracted from site, and having time in service (TS) of 2 to 294 years (with reference to 2021, the year of extraction). 240 MD measurements were conducted at varying MC values of 7% to 212%. The obtained MD profiles showed for all TS a slight decrease in drilling resistance when increasing MC. However, from the MD signals it is possible to reliably detect the areas affected by biodegradation phenomena (e.g. bacterial decay) along the drilling depth, regardless the MC of the segment or its gradient along the drilling depth. The present study contributes to research aiming at utilizing (in-situ) MD techniques for reliably assessing and quantifying decay and to be used in remaining service life planning of wooden foundation piles.Bio-based Structures & Material

Characterisation of bacterial decay effects on wooden foundation piles across various historical periods

In the historic city centre of Amsterdam (NL), the predominant foundation system is comprised of wooden piles. Due to their placement below the water table, these foundations are susceptible to bacterial decay. This study aims to investigate and compare various methods for characterizing decay patterns within the cross sections of piles retrieved from two bridges in Amsterdam. The examined piles span different construction years: three originate from 1727, four from 1886, and two from 1922. Following extraction, the piles were transported to TU Delft Stevin II Laboratory, where they underwent further subdivision into three segments, each representing the head, middle, and tip, resulting in a total of 27 segments. The effects of bacterial decay were characterised by performing micro-drilling measurements, small-scale material and compressive tests on prismatic samples extracted from the segments' cross sections, computed tomography scans, and light microscopy observations. Microscopic examination revealed severe degradation in all segments dating back to 1727, extending 20–50 mm from their surface. This outcome was also confirmed by the other adopted methods: the corresponding prisms had large moisture contents and poor mechanical properties, while low basic densities and drilling amplitudes were obtained from CT scans and micro-drilling measurements, respectively. On the contrary, the internal sections of the 1727 segments exhibited no evidence of decay and demonstrated properties consistent with those observed in sound segments from 1886 and 1922. Finally, the observed gradients of density, strength, and stiffness were well correlated with micro-drilling measurements, which can therefore be reliably used as on-site assessment method to reconstruct the properties of the piles.Bio-based Structures & Material