Identification of pollen-expressed pectin methylesterase inhibitors in Arabidopsis

AbstractPectin methylesterases (PMEs) play an essential role during plant development by affecting the mechanical properties of the plant cell wall. Previous work indicated that plant PMEs may be subject to post-translational regulation. Here, we report the analysis of two proteinaceous inhibitors of PME in Arabidopsis thaliana (AtPMEI1 and 2). The functional analysis of recombinant AtPMEI1 and 2 proteins revealed that both proteins are able to inhibit PME activity from flowers and siliques. Quantitative RT-PCR analysis indicated that expression of AtPMEI1 and 2 mRNAs is tightly regulated during plant development with highest mRNA levels in flowers. Promotor::GUS fusions demonstrated that expression is mostly restricted to pollen

Natürlicher Verlauf des Marburger Patientenguts an operierten Plattenepithelkarzinomen des Harntrakts

„Natürlicher Verlauf des Marburger Patientengutes an operierten Plattenepithelkarzinomen des Harntraktes“ Einleitung: Plattenepithelkarzinome (SCC) und plattenepitheliale Karzinome des Harntraktes sind in Westeuropa selten. Die Therapie des Plattenepithelkarzinoms ist daher eng an diejenige des Urothelkarzinoms angelehnt. Zielsetzung der Arbeit war die Charakterisierung der Tumorbiologie und -Prognose, sowie Evaluierung der Therapie von SCC im Kontext aktueller Behandlungsstrategien bei Karzinomen des Harntraktes. Material und Methoden: Das Patientengut der Klinik für Urologie des Universitätsklinikums Marburg vom 30.5.1989 bis 30.9.2004 wurde retrospektiv analysiert. 53 Patienten mit Plattenepithelkarzinomen und plattenepithelial differenzierten Karzinomen des Harntraktes wurden identifiziert und hinsichtlich Tumorstadium, Therapie und Überleben nachbeobachtet. Ergebnisse: Der Anteil der Plattenepithelkarzinome am Gesamtkollektiv betrug für Harnblasentumoren 2% (42/1573) und 7% (11/130) für Nierenbeckenkarzinome. Es zeigte sich eine deutliche Tendenz zu tief infiltrierenden Tumorstadien, mit Überwiegen des Stadiums pT3 (50%). In 60% der Fälle war das postoperative Grading G3. Die Tendenz zur systemischen Tumoraussaat war mit 26% nodal positiven, bzw. fernmetastasierten Karzinomen gering. Die 5-Jahres-Gesamtüberlebensrate betrug 26% für Harnblasenkarzinome (tumorspezifisch 46%), bei einem medianen Überleben von 11,13 Monaten. 3 von 4 Patienten mit einem Harnblasentumor pT2N0 konnten durch radikale Zystektomie geheilt werden. Bei Tumorlokalisation im Nierenbecken beobachtete man ein medianes Überleben von 7,25 Monaten und eine tumorspezifische 5-JÜR von 30%. T-Stadium und Lymphknotenstatus konnten als Parameter mit Einfluss auf die Prognose der Patienten identifiziert werden. Adjuvante Therapieformen wurden nur vereinzelt eingesetzt, zeigten jedoch häufig ein therapeutisches Ansprechen. Schlussfolgerung: Plattenepithelkarzinome und plattenepitheliale Urothelkarzinome des Harntraktes zeigen im Vergleich zum Urothelkarzinom eine eigenständige Tumorbiologie und eine schlechtere Prognose. Das Überleben korreliert eng mit der lokalen Tumorausdehnung und macht somit eine frühzeitige radikalchirurgische Therapie erforderlich. Analog zum Urothelkarzinom können adjuvante Radio-/Chemotherapie den Therapieerfolg unterstützen

Galactomannan Does Not Precede Major Signs on a Pulmonary Computerized Tomographic Scan Suggestive of Invasive Aspergillosis in Patients with Hematological Malignancies

Background. Detection of serum galactomannan (GM) antigen and presence of the halo sign on a pulmonary computerized tomographic (CT) scan have a high specificity but a low sensitivity to diagnose invasive aspergillosis (IA) in patients at risk for this disease. To our knowledge, the relationship between the time at which pulmonary infiltrates are detected by CT and the time at which GM antigens are detected by enzyme immunoassay (EIA) has not been studied. Methods. In a prospective study, tests for detection of GM were performed twice weekly for patients with hematological malignancies who had undergone hematopoetic stem cell transplantation (HSCT) or had received induction and/or consolidation chemotherapy. A pulmonary CT scan was performed once weekly. Infiltrates were defined as either major or minor signs. IA was classified as proven, probable, or possible, in accordance with the definition stated by the European Organization for Research and Treatment of Cancer-Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group. Results. We analyzed 161 episodes of infection in 107 patients (65 allogeneic HSCT recipients, 30 autologous HSCT recipients, and 66 induction and/or consolidation chemotherapy recipients). A total of 109 episodes with no IA, 32 episodes with possible IA, and 20 episodes with probable or proven IA were identified. Minor pulmonary signs were detected by CT in 70 episodes (43%), and major pulmonary signs were detected by CT in 11 episodes (7%). Univariate and multivariate analyses revealed no significant association between detection of GM by EIA and detection of abnormal pulmonary signs by CT. A significant association was found between GM levels and receipt of piperacillin-tazobactam. GM test results were not positive before major signs were seen on CT images. Only 7 (10%) of 70 patients with minor pulmonary signs had positive GM test results before detection of the greatest pathologic change by CT. Conclusions. We show that detection of GM by EIA does not precede detection of major lesions by pulmonary CT. In the clinical setting, the decision to administer mold-active treatment should based on detection of new pulmonary infiltrates on CT performed early during infection, rather than on results of EIA for detection of G

Extracellular invertase is involved in the regulation of clubroot disease in Arabidopsis thaliana

Clubroot disease of Brassicaceae is caused by an obligatebiotrophic protist,Plasmodiophora brassicae. During root galldevelopment, a strong sink for assimilates is developed. Amongother genes involved in sucrose and starch synthesis and degra-dation, the increased expression of invertases has been observedin a microarray experiment, and invertase and invertase inhibitorexpression was confirmed using promoter::GUS lines ofArabi-dopsis thaliana. A functional approach demonstrates that inver-tases are important for gall development. Different transgeniclines expressing an invertase inhibitor under the control of tworoot-specific promoters,Pyk10andCrypticT80, which results inthe reduction of invertase activity, showed clearly reduced clu-broot symptoms in root tissue with highest promoter expression,whereas hypocotyl galls developed normally. These resultspresent the first evidence that invertases are important factorsduring gall development, most probably in supplying sugars tothe pathogen. In addition, root-specific repression of invertaseactivity could be used as a tool to reduce clubroot symptoms

The Malpaisillo Formation: A sequence of explosive eruptions in the mid to late Pleistocene (Nicaragua, Central America)

The subduction-related volcanic front in Nicaragua consists of the Tertiary “Coyol” member in the eastern highlands and the Quaternary to recent volcanic arc within the Nicaraguan depression. Although the Holocene to recent explosive volcanism has been studied extensively no detailed work has been done on the products of explosive volcanism from Quaternary volcanic complexes comprising also the Malpaisillo and Monte Galán Calderas, the focus of this study. The 11 km-wide Malpaisillo Caldera and ~3.5 km-wide Monte Galán Caldera, located ~50 km northwest of Managua, are surrounded by tens of meters of rhyolitic tephras. These pyroclastic flow and fall deposits extend proximally at least 11 km to the southeast and 23 km to the southwest, with observed depositional thicknesses of >16 m for a single ignimbrite unit (or >25 m for the entire section). Distal deposits are found as far as 350 km offshore in the Pacific. At least twelve highly explosive large-volume eruptive phases with corresponding tephra deposits (LPT = La Paz Centro Tephra, PPT = Punta de Plancha Tephra, LCbT = Lower Chibola Tephra, GT = Guacucal Tephra, UCbT = Upper Chibola Tephra, FeT = La Fuente Tephra, ST = Sabanettas Tephra, MgT = Miralago Tephra, ToT = Tolapa Tephra, LMT, MMT, UMT = Lower, Middle, and Upper Maderas Negras Tephras) are distinguished based on geochemical correlations and similar depositional characteristics. Radiometric 40Ar/39Ar ages indicate that most activity related to the large Malpaisillo Caldera occurred between ~570 and ~420 ka. The large Pleistocene Malpaisillo and Monte Galán Calderas are characterized by a long- lived history and, if evolved, a distinctly alkaline (K2O = 2.3–3.8 wt%; Na2O = 4.0– 4.9 wt%) geochemical signature compared to the other Nicaraguan tephra deposits. As a result, the previously defined Malpaisillo Formation has been considerably extended and revised. Our findings contribute to fill a considerable gap in the long- term eruptive history of Nicaraguan volcanoes, with prominent implications for volcanic hazard evaluation for Nicaragua

Managed and Continuous Evolution of Dependable Automotive Software Systems / Andreas Rausch, Oliver Brox, Axel Grewe, Marcel Ibe, Stefanie Jauns-Seyfried, Christoph Knieke, Marco Körner, Steffen Küpper, Malte Mauritz, Henrik Peters, Arthur Strasser, Martin Vogel, Norbert Weiss

Automotive software systems are an essential and innovative part of nowadays connected and automated vehicles. Automotive industry is currently facing the challenge to re-invent the automobile. Consequently, automotive software systems, their software systems architecture, and the way we engineer those kinds of software systems are confronted with major challenges: managing complexity, providing flexibility, and guaranteeing dependability of the desired automotive software systems and the corresponding engineering process. In this paper we will present an improved and sophisticated engineering approach. Our approach is based on the managed and continuous evolution of dependable automotive software systems. It helps engineers to manage system complexity based on continous engineering processes to iteratively evolve automotive software systems and therby guarantee the required dependability issues. Based on a running sample, we will present and illustrate the main assets of the proposed engineering approach for managed and continuous evolution of dependable automotive software systems

Managed and Continuous Evolution of Dependable Automotive Software Systems / Andreas Rausch, Oliver Brox, Axel Grewe, Marcel Ibe, Stefanie Jauns-Seyfried, Christoph Knieke, Marco Körner, Steffen Küpper, Malte Mauritz, Henrik Peters, Arthur Strasser, Martin Vogel, Norbert Weiss

Automotive software systems are an essential and innovative part of nowadays connected and automated vehicles. Automotive industry is currently facing the challenge to re-invent the automobile. Consequently, automotive software systems, their software systems architecture, and the way we engineer those kinds of software systems are confronted with major challenges: managing complexity, providing flexibility, and guaranteeing dependability of the desired automotive software systems and the corresponding engineering process. In this paper we will present an improved and sophisticated engineering approach. Our approach is based on the managed and continuous evolution of dependable automotive software systems. It helps engineers to manage system complexity based on continous engineering processes to iteratively evolve automotive software systems and therby guarantee the required dependability issues. Based on a running sample, we will present and illustrate the main assets of the proposed engineering approach for managed and continuous evolution of dependable automotive software systems

Walking through volcanic mud : the 2,100 year-old Acahualina footprints (Nicaragua) II: the Acahualina people, environmental conditions and motivation

We analyzed bare human footprints in Holocene tuff preserved in two pits in the Acahualinca barrio in the northern outskirts of Managua (Nicaragua). Lithology, volcanology, and age of the deposits are discussed in a companion paper (Schmincke et al. Bull Volcanol doi: 10.1007/s00445-008-0235-9, 2008). The footprint layer occurs within a series of rapidly accumulated basaltic–andesitic tephra that is regionally correlated to the Masaya Triple Layer Tephra. The people were probably trying to escape from a powerful volcanic eruption at Masaya Caldera 20 km farther south that occurred at 2.1 ka BP. We subdivided the swath of footprints, up to 5.6 m wide, in the northern pit (Pit I) into (1) a central group of footprints made by about six individuals, the total number being difficult to determine because people walked in each other’s footsteps one behind the other and (2) two marginal groups on either side of the central group with more widely spaced tracks. The western band comprises tracks of three adjacent individuals and an isolated single footprint farther out. The eastern marginal area comprises an inner band of deep footprints made by three individuals and, farther out, three clearly separated individuals. We estimate the total number of people as 15–16. In the southern narrow and smaller pit (Pit II), we recognize tracks of ca. 12 individuals, no doubt made by the same group. The group represented in both pits probably comprised male and female adults, teenagers and children based on differences in length of footprints and of strides and depth of footprints made in the soft wet ash. The smallest footprints (probably made by children) occur in the central group, where protection was most effective. The footprint layer is composed of a lower 5–15-cm thick, coarse-grained vesicle tuff capped by a medium to fine-grained tuff up to 3 cm thick. The surface on which the people walked was muddy, and the soft ash was squeezed up on the sides of the foot imprints and between toes. Especially, deep footprints are mainly due to local thickening of the water-rich ash, multiple track use, and differences in weight of individuals. The excellent preservation of the footprints, ubiquitous mudcracks, sharp and well-preserved squeeze-ups along the margins of the tracks and toe imprints, and the absence of raindrop impressions all suggest that the eruption occurred during the dry season. The people walked at a brisk pace, as judged from the tight orientation of the swath and the length of the strides. The directions of a major erosional channel in the overlying deposits that probably debouched into Lake Managua and the band of footprints are strictly parallel, indicating that people walked together in stride along the eastern margin of a channel straight toward the lake shore, possibly a site with huts and/or boats for protection and/or escape