Development of a nucleic acid lateral flow immunoassay for simultaneous detection of Listeria spp. and Listeriamonocytogenes in food

We present a new nucleic acid lateral flow immunoassay (NALFIA) for the assessment of listeria contamination. The detection procedure starts with enrichment of sample in Half Fraser broth (24 h). Following isolation of DNA, a duplex PCR is performed with two labelled primer sets, one generic and directed to a specific sequence of the gene encoding 16S rRNA from Listeria spp. and the other specific and directed to a part of the prfA gene encoding the central virulence gene regulator from the food pathogen Listeria monocytogenes (3.5 h). The PCR solution is directly added to the one-step assay device and the appearance of a grey/black line is indicative of the presence of specific amplicons (max 15 min). In all tests performed, the method correctly identified L. monocytogenes and strains of Listeria spp. PCR material of over 20 food samples was tested by NALFIA. The method proved to be useful for the detection of L. monocytogenes in different kinds of food sample

Stellar laboratories. V. The Xe VI ultraviolet spectrum and the xenon abundance in the hot DO-type white dwarf RE0503-289

For the spectral analysis of spectra of hot stars with a high resolution and high signal-to-noise ratio (S/N), advanced non-local thermodynamic equilibrium (NLTE) model atmospheres are mandatory. These are strongly dependent on the reliability of the atomic data that are used for their calculation. Reliable Xe VI oscillator strengths are used to identify Xe lines in the ultraviolet spectrum of the DO-type white dwarf RE0503-289 and to determine its photospheric Xe abundance. We publish newly calculated oscillator strengths that are based on a recently measured Xe VI laboratory line spectrum. These strengths were used to consider their radiative and collisional bound-bound transitions in detail in our NLTE stellar-atmosphere models to analyze Xe VI lines exhibited in high-resolution and high S/N UV observations of RE0503-289. We identify three hitherto unknown Xe VI lines in the ultraviolet spectrum of RE0503-289 and confirm the previously measured photospheric Xe abundance of this white dwarf (log Xe = -4.2 +/- 0.6). Reliable measurements and calculations of atomic data are prerequisite for stellar-atmosphere modeling. Observed Xe VI line profiles in the ultraviolet spectrum of the white dwarf RE0503-289 were well reproduced with the newly calculated Xe VI oscillator strengths.Comment: 3 pages, 4 figure

Temperature and Kinematics of CIV Absorption Systems

We use Keck HIRES spectra of three intermediate redshift QSOs to study the physical state and kinematics of the individual components of CIV selected heavy element absorption systems. Fewer than 8 % of all CIV lines with column densities greater than 10^{12.5} cm^{-2} have Doppler parameters b < 6 km/s. A formal decomposition into thermal and non-thermal motion using the simultaneous presence of SiIV gives a mean thermal Doppler parameter b_{therm}(CIV) = 7.2 km/s, corresponding to a temperature of 38,000 K although temperatures possibly in excess of 300,000 K occur occasionally. We also find tentative evidence for a mild increase of temperature with HI column density. Non-thermal motions within components are typically small (< 10 km/s) for most systems, indicative of a quiescent environment. The two-point correlation function (TPCF) of CIV systems on scales up to 500 km/s suggests that there is more than one source of velocity dispersion. The shape of the TPCF can be understood if the CIV systems are caused by ensembles of objects with the kinematics of dwarf galaxies on a small scale, while following the Hubble flow on a larger scale. Individual high redshift CIV components may be the building blocks of future normal galaxies in a hierarchical structure formation scenario.Comment: submitted to the ApJ Letters, March 16, 1996 (in press); (13 Latex pages, 4 Postscript figures, and psfig.sty included

Nucleic Acid Lateral Flow Immunoassay for the Detection of Pathogenic Bacteria from Food

Nucleic acid lateral flow immunoassay (NALFIA) is a method combining molecular biological principle of detection with immunochemical principle of visualisation. Following isolation of DNA from the sample, a duplex PCR with two primer sets, of which one was labelled with biotin and the other with digoxigenin or fluorescein, respectively, was performed. The PCR solution and carbon particles conjugated with avidin are directly added to the nitrocellulose membrane with two test lines of immobilised antibodies specific for digoxigenin and fluorescein. The appearance of a black line indicates the presence of specific amplicon. We would like to present the NALFIA for the simultaneous detection of L. monocytogenes in particular and the genus Listeria in general, in food. Bacteria from the genus Listeria frequently contaminate a large variety of foods. Occurrence of Listeria strains in food may indicate errors in good hygienic and manufacturing practice, only L. monocytogenes is a significant human and animal pathogen responsible for the serious illness listeriosis. Conventional microbiological methods for L. monocytogenes detection are laborious and take several days to achieve a confirmed identification

EC 11481-2303 - A Peculiar Subdwarf OB Star Revisited

EC 11481-2303 is a peculiar, hot, high-gravity pre-white dwarf. Previous optical spectroscopy revealed that it is a sdOB star with an effective temperature (Teff) of 41790 K, a surface gravity log(g)= 5.84, and He/H = 0.014 by number. We present an on-going spectral analysis by means of non-LTE model-atmosphere techniques based on high-resolution, high-S/N optical (VLT-UVES) and ultraviolet (FUSE, IUE) observations. We are able to reproduce the optical and UV observations simultaneously with a chemically homogeneous NLTE model atmosphere with a significantly higher effective temperature and lower He abundance (Teff = 55000 K, log (g) = 5.8, and He / H = 0.0025 by number). While C, N, and O appear less than 0.15 times solar, the iron-group abundance is strongly enhanced by at least a factor of ten.Comment: 8 pages, 11 figure

Neutron wave packet tomography

A tomographic technique is introduced in order to determine the quantum state of the center of mass motion of neutrons. An experiment is proposed and numerically analyzed.Comment: 4 pages, 3 figure

Stellar laboratories. IX. New Se V, Sr IV - VII, Te VI, and I VI oscillator strengths and the Se, Sr, Te, and I abundances in the hot white dwarfs G191-B2B and RE 0503-289

To analyze spectra of hot stars, advanced non-local thermodynamic equilibrium (NLTE) model-atmosphere techniques are mandatory. Reliable atomic data is for the calculation of such model atmospheres. We aim to calculate new Sr IV - VII oscillator strengths to identify for the first time Sr spectral lines in hot white dwarf (WD) stars and to determine the photospheric Sr abundances. o measure the abundances of Se, Te, and I in hot WDs, we aim to compute new Se V, Te VI, and I VI oscillator strengths. To consider radiative and collisional bound-bound transitions of Se V, Sr IV - VII, Te VI, and I VI in our NLTE atmosphere models, we calculated oscillator strengths for these ions. We newly identified four Se V, 23 Sr V, 1 Te VI, and three I VI lines in the ultraviolet (UV) spectrum of RE0503-289. We measured a photospheric Sr abundance of 6.5 +3.8/-2.4 x 10**-4 (mass fraction, 9500 - 23800 times solar). We determined the abundances of Se (1.6 +0.9/-0.6 x 10**-3, 8000 - 20000), Te (2.5 +1.5/-0.9 x 10**-4, 11000 - 28000), and I (1.4 +0.8/-0.5 x 10**-5, 2700 - 6700). No Se, Sr, Te, and I line was found in the UV spectra of G191-B2B and we could determine only upper abundance limits of approximately 100 times solar. All identified Se V, Sr V, Te VI, and I VI lines in the UV spectrum of RE0503-289 were simultaneously well reproduced with our newly calculated oscillator strengths.Comment: 26 pages, 5 figure

Fractal geometry of critical Potts clusters

Numerical simulations on the total mass, the numbers of bonds on the hull, external perimeter, singly connected bonds and gates into large fjords of the Fortuin-Kasteleyn clusters for two-dimensional q-state Potts models at criticality are presented. The data are found consistent with the recently derived corrections-to-scaling theory. However, the approach to the asymptotic region is slow, and the present range of the data does not allow a unique identification of the exact correction exponentsComment: 7 pages, 8 figures, Late

The metal line systems in HS1700+6416: evidence for inhomogeneities

We present a high S/N ratio optical spectrum of the bright quasar HS1700+6416. These data usefully complement the UV HST data from Vogel and Reimers (1995). We analyse the metal line systems using photo-ionization models allowing for inhomogeneities in the gas. The models are able to reproduce within a factor of two the large N(HeI)/N(HI) ratio together with the mean column densities of the heavy element species observed in the z=2 systems. The density contrast between low and high density regions is of the order of 30. Although the [O/C] abundance ratio seems slighly larger than solar, firm conclusion should await higher spectral resolution data. A break at the HeII ionization limit of a factor of ten is acceptable in the ionizing spectrum. Abundances are found to be about 0.08 solar. This together with other determinations from the literature indicates that there is evolution in the metal content of metal line systems with redshift: [Mg/H] is slighly below solar at z=0.7 and [C/H]=0.1-0.01 at z=2. We detect an associated CIV system at z=2.7126 in which there is some evidence for the presence of NeVIII, probably NeVI, NeVII, and possibly SiXII

Quantum Mechanics in Non-Inertial Frames with a Multi-Temporal Quantization Scheme: II) Non-Relativistic Particles

The non-relativistic version of the multi-temporal quantization scheme of relativistic particles in a family of non-inertial frames (see hep-th/0502194) is defined. At the classical level the description of a family of non-rigid non-inertial frames, containing the standard rigidly linear accelereted and rotating ones, is given in the framework of parametrized Galilei theories. Then the multi-temporal quantization, in which the gauge variables, describing the non-inertial effects, are not quantized but considered as c-number generalized times, is applied to non relativistic particles. It is shown that with a suitable ordering there is unitary evolution in all times and that, after the separation of center of mass, it is still possible to identify the inertial bound states. The few existing results of quantization in rigid non-inertial frames are recovered as special cases