Efficacy of silver and gold nanoparticles obtained from vermiwash: In vitro study on antimicrobial and antidiabetic activities

Emerging nanobiotechnology has provided innovative techniques to synthesize nanoparticles through biological methods to explore the potentialities of biological sources like phytoextracts, microbes, animal secretions and excretion. This research studies the potential of vermiwash to synthesize the silver and gold nanoparticles and evaluate its in vitro effect of antimicrobial   and antidiabetic activities. The characterization of the nanoparticles was analyzed through various techniques. Ultraviolet (UV)-Visible spectroscopy showed the maximum absorption spectrum at 413 nm for silver and 541 nm for gold nanoparticles. Fourier transform infrared spectroscopy (FTIR) revealed the reducing agent involved in nanoparticles synthesis. Scanning electron microscope (SEM) images revealed the size of the silver and gold nanoparticles as 24 nm and 50 nm, respectively. Energy dispersive X-ray (EDAX) analysis revealed the elemental composition of the synthesized nanoparticles. X-ray diffraction (XRD) analysis confirmed the crystalline nature of the nanoparticles that displayed the preferential orientation of the crystals toward the (111) plane.  Antimicrobial activity was assessed using the resazurin assay method.  A minimum inhibitory concentration (MIC) of less than 7.8 µg was observed in Staphylococcus aureus and Klebsiella pneumoniae. In the antifungal activity, MIC at 250 µg was noted in Mucor sp. and Candida albicans. Antidiabetic activity was assessed by α-amylase and α-glucosidase inhibitory assay. IC50 of α-amylase and α-glucosidase activity of the silver nanoparticles was noted as 218 and 221 µg/mL, respectively. IC 50 value for the enzymatic assay dose-dependently confirmed the effect. Conclusively biosynthesized nanoparticles from vermiwash showed potential efficiency of antibacterial, antifungal and antidiabetic activities

Xpert MTB/RIF assay for the diagnosis of pulmonary and extra pulmonary tuberculosis

Detecting patients with active Tuberculosis (TB) disease is an important component of tuberculosis control programs, as early diagnosis and treatment of tuberculosis is essential in reducing the morbidity, mortality and the escalating costs associated with advanced disease. We conducted this study to access the usefulness of Gene Xpert MTB/RIF assay technique in the diagnosis of tuberculosis and rifampicin (Rif) resistance. We retrospectively reviewed the clinical records and rapid diagnostic results of all patients with suspected Tuberculosis who visited the pulmonary clinic of Government Rajaji Hospital (GRH), Madurai from January 2017 to June 2017. Various pulmonary and extra pulmonary specimens were collected under aseptic precautions. These Xpert MTB/RIF samples were processed according to the manufacturer’s specifications. A total of 764 consecutive pulmonary and extra pulmonary clinical samples were included. Out of 764 samples, 136 (18%) tested positive for M.tuberculosis. Of these 136 positive samples, 124 (91.2%) were found to be Rif sensitive and 10 (7.4%) were found to be Rif resistant. Rif resistant cases were referred for initiation of MDR treatment. This study shows that GenXpert is useful in the early diagnosis of Rif resistance in both pulmonary and extrapulmonary specimens

A pilot study on the isolation and biochemical characterization of Pseudomonas from chemical intensive rice ecosystem

In recent times, there has been a renewed interest in the search of plant growth promoting rhizobacteria (PGPR) for sustainable crop production. Rice is an economically important food crop, which is subjected to infection by a host of fungal, viral and bacterial pathogens. In this study, an attempt was made to isolate Pseudomonas spp., a potent PGPR in the rhizosphere. Through appropriate microbiological and biochemical methods, the study demonstrated the presence of fluorescent and nonfluorescent Pseudomonads in the rhizosphere of chemical intensive rice growing environments. Augmentation of such PGPR including, Pseudomonads in the rice ecosystems will ensure a healthy micro climate for rice.Key words: Pseudomonas, rice, plant growth promoting rhizobacteria (PGPR)

Phocomelia: is it time to retrospect, regulate and rescue? a case report

Phocomelia is an extremely rare congenital anomaly which presents as, the proximal part of the limb (humerus or femur, radius or tibia, ulna or fibula) being absent or markedly hypoplastic, with normal or near normal hand or foot. True phocomelia presents as the total absence of the intermediate segments of the limb, with the hand or foot directly attached to the trunk. Presented here is a case of phocomelia in an aborted foetus, with no maternal history of thalidomide exposure in her pregnancy and for whom evaluation of other family members/ siblings failed to reveal any substantial abnormality. The differential diagnosis and the significance of reporting of serious adverse drug reactions are discussed

Tuna drift gillnet fishery at Chennai, Tamil Nadu- an update

The present study describes the status of multiday drift gillnet fishery for tuna from Chennai fishing harbour based on data for the years 2016 – 2017. The data is also compared with that during 1999- 2006. Both the craft and gear increased in size with consequent extension of fishing grounds and increase in the number of days/ fishing trip. The size of the boats increased to 20-23 m OAL from 11-12 m OAL and weight of the gear from 1 to more than 6 t. Annual average catch increased to 8523 t during 2016-2017 from 595 t during 1999-2006. Average catch per unit effort was 8310 kg as against 730 kg during 1999-2006. Yellowfin tuna, Thunnus albacares and Skipjack tuna, Katsuwonus pelamis were the dominant species. The stock position of skipjack tuna and yellowfin tuna vis-àvis the three indicators indicated that the percentage of mature yellowfin tuna in the catch in 2017 was 68%, fish in optimum length 35% and mega-spawners 33% whereas in skipjack tuna the respective percentages were 99.5, 21.1 and 79.1. Problems and prospects of multiday tuna drift gillnet fishery are also discussed

A comparative assessment of the impact of computer assisted learning of knowledge and skill based competencies among undergraduate students in a medical college in South India

Background: Pharmacology has always been one subject ultimatum and a cornerstone in building up competency, based on applied therapeutics among medical graduates in prescribing and practicing skills. A focus on an integrated strategical approach towards teaching pharmacology for better proficiency in achieving clinical skills is mandatory. Thus, this study has been done to assess the impact and retainment of overall knowledge gained on various domains, based on a revised method of teaching in pharmacology.Methods: An observational, questionnaire based comparative study was done in a medical college, in Chennai. The study included two group of students, one who have completed their pharmacology course based on the revised pattern of teaching with a comparative group of students who have not been exposed to the revised pattern of teaching. A feedback survey was also done.Results: The study results indicated that the average scoring based on the questionnaire was 84% with the students who have been exposed to revised pattern of teaching in comparison to the average scoring of 70% with the students who have not been exposed to the new pattern of teaching. The average scoring of the students who were found to agree to several questions on the new method of teaching was 74% and 22% of the students were found to disagree. 4% of students were non responders.Conclusions: The ultimate improvement in the outcome of health care education can be achieved through appropriate skill building through a simple strategical modification of the regular teaching pattern with an integrated approach involving pharmacologists and clinicians. A refresher course in pharmacology in the final year curriculum and CRRI involving pharmacologists is mandatory

Manipulation of fatty acids in the estuarine clam Meretrix casta (Gmelin, 1791) by supplementation with the microalgal diet, Isochrysis galbana

The present study evaluated the changes in fatty acid profile of the estuarine clam Meretrix casta, an important food organism used in the larval rearing of scyllarid lobsters, after supplementation with the microalgal species Isochrysis galbana. The uptake and assimilation of lipids from the microalgal feed were verified by gas chromatographic analysis of fatty acids in the clam tissues after eight days of feeding with I. galbana. Increase in concentrations of polyunsaturated fatty acids (PUFA), in particular docosahexaenoic acid (DHA, C22:6n3) and C18:2n-6, was observed in clams supplemented with I. galbana. Changes in monounsaturated fatty acid (MUFA) composition were less marked and related to the increasing proportions of C18:1, after supplementation. Feeding with I. galbana also induced a decrease in the proportion of saturated fatty acids, which was related to decrease in proportions of both C16:0 and C18:0. Although the fatty acid composition showed significant differences, the gross lipid content of the clam tissues did not seem to be excessively influenced by the algal feeding. Tissues from clams supplemented with I. galbana are being evaluated as feed for sand lobster larval trials

The effects of intrinsic noise on the behaviour of bistable cell regulatory systems under quasi-steady state conditions

We analyse the effect of intrinsic fluctuations on the properties of bistable stochastic systems with time scale separation operating under1 quasi-steady state conditions. We first formulate a stochastic generalisation of the quasi-steady state approximation based on the semi-classical approximation of the partial differential equation for the generating function associated with the Chemical Master Equation. Such approximation proceeds by optimising an action functional whose associated set of Euler-Lagrange (Hamilton) equations provide the most likely fluctuation path. We show that, under appropriate conditions granting time scale separation, the Hamiltonian can be re-scaled so that the set of Hamilton equations splits up into slow and fast variables, whereby the quasi-steady state approximation can be applied. We analyse two particular examples of systems whose mean-field limit has been shown to exhibit bi-stability: an enzyme-catalysed system of two mutually-inhibitory proteins and a gene regulatory circuit with self-activation. Our theory establishes that the number of molecules of the conserved species are order parameters whose variation regulates bistable behaviour in the associated systems beyond the predictions of the mean-field theory. This prediction is fully confirmed by direct numerical simulations using the stochastic simulation algorithm. This result allows us to propose strategies whereby, by varying the number of molecules of the three conserved chemical species, cell properties associated to bistable behaviour (phenotype, cell-cycle status, etc.) can be controlled.Comment: 33 pages, 9 figures, accepted for publication in the Journal of Chemical Physic

Minimum Legal Size proposed for commercially exploited marine finfish and shellfish resources of Tamil Nadu

Marine fisheries in Tamil Nadu have undergone tremendous change in terms of fishing pattern, fishing method, extension of fishing grounds, composition of fish catch and consequent increase in the total fish catch in recent years. The recent demand from industries involved in fish meal and fish oil encourages targeted fishing for by-catch resulting in heavy landing of low value by-catch in certain places along Tamil Nadu coast. These by-catch are often dominated by juveniles of many commercially important marine finfishes and shell fishes. So it warrants some caution and intervention. One of the methods to discourage the indiscriminate exploitation of juveniles is to impose a Minimum Legal Size (MLS) which is the size at which a particular species can be legally retained if caught. The advantage of a MLS is that it aids in the control of two major problems in the fisheries management, growth overfishing and recruitment overfishing either by increasing the minimum size of harvest or by increasing or maintaining the size of the spawning stock. The most common method of increasing the reproductive output through the use of size limits is to set the minimum size at which the females become sexually mature. As the individuals of a species do not attain sexual maturity at the same size, it can be a size at which higher proportions are mature